| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is acyII [H]
Identifier: 94314554
GI number: 94314554
Start: 2393097
End: 2395535
Strand: Reverse
Name: acyII [H]
Synonym: Rmet_5635
Alternate gene names: 94314554
Gene position: 2395535-2393097 (Counterclockwise)
Preceding gene: 94314555
Following gene: 94314549
Centisome position: 92.85
GC content: 65.85
Gene sequence:
>2439_bases ATGTTTCGCCCGACCCGTTCCCTGCGGCGCACCGCCGTCGCCGCGGCATGGCTTGCCGCCGCATCCACCGCCCCCGCGCT GGCGGCCGGCCCGGACAAGTTCGCCGTCCCCGGACTGGAAAAGCCTGCAACGGTATTGATCGACCGCTGGGGCGTGCCGC ATATCTACGCCAACACGCTCTATGACGCGTTCTTCGTGCAAGGCTTCGTGGCCGCGCGGGACCGGCTGTTCCAGATCGAT CTGTGGCGCAAGCGTGGGCTGGGCGAAATGGCGAAAGACTTCGGTCCGGCCTATGTGGAAGGCGATCGCATGGCGCGCGC CGTGTTGTTCCGTGGCGATATGTACCGCGAATGGCTGGCCTACGGATCGGATGCCAAGCGTGTGGCCGAGGCCTTCGTGG CGGGCGTCAATGCGTACATCGCGCAGACCGAGGCCCATCCGGAACTGCTGCCTCCCGAGTTCAAGCTGATCAACTACAAG CCGTCGCGCTGGCGTGCCGAGGATATCGTGCGTATCCGTCATCACGGGCTGACGTTGAACCTCACTGGCGAGATCGACCG GGCGCAGATCTTCTGCTACGCGAAGCCGAACGCGGTACGGGCGGACTGGGTTCGCCGCGAACTCGATCCGCAGATCGAGC CGAAGGTGCCAGACGGGCTCGACCCGTGCACGGTCCCCGCTGCCGAGTTGCGCAAGGCGTATGAGCTTGCCACGGCCGCG CCGCGCTTCCCGAAGGACGTGTGGCAATCGGGCACGCGTGCCGAGAACATCCCGGTGGACCAGCTTTATGCGACGGTCGA TGCGTCGAGCGATACCGCACGCAGCCTGGGGTCGAACAACTGGGTGATCGCGGGCAGCCGCACCGCCACGGGGCGACCGA TCCTGGCCAACGATCCGCATCGAGCGCACGGTGCGCCGAGCCTGCGCTATATCTCCCACCTGAACGCGCCGGGCCTGTCA GTAATCGGCGCGGGCGAGCCGTTCCTGCCCGGTATCTCCATCGGTCACAACGGCACGATTGCTTTCGGCCTGACGCGGTT CTACATGGACCAGGAAGACCTCTATGCCTATGAGACCGATCCCGCCCAGCCGCACGAATACAAGTACAAGGGCCGCTGGG AGCCGATGGAGACGGTGACCGAGCAGATCTCAGTGCGTGGCGAGGCGACGCCACGCAAGGTCACGATCGACTTCACGCGC CACGGCCCGGTGCTCTATGCCGACTCGAAGAACAACCGCGCCTGGGCCCTGCGCGCGGCATGGCTTGACCTTGGCATGGC ACCGTATTTCGGGTCGATGGATTACATGCGTGCCCAGAACTGGGATCAGTTCCGCGCGGCGATGAACCGCTGGGGCGCTC CTGGCGAGAACCAGGTCTATGCGGACCGCTTCGGCAACATCGGCTGGCTCCCGGGCGGGTTGACCGTCAATCGGCCGAAC TGGGATGGCCTGATGCCGGTGCCCGGGGACGGACGCTACGAATGGGCCGGCTATCGCAACATGGACGAACTGCCGTGGGC GTTTAATCCGGCGCCGGGATTCGTGGTCACTGCCAACGAGAACACGATCCCGCCCAATCACCCGGCAGCCAAGCTCGGCG TCGGCTACGAATGGGCCGAAAGCTCGCGCGCACGTCGGCTCAGGGCGCTGGTGTCGGCCAATCCGCGCAGTTCGGTGGAG GACTCGATGGCATGGCAGAACGACACGGTGTCATTGCCCGCGCAGCGGATGGTGACGTTGCTCAAGCCGCTCGCCAGCAG CGATGCGCAGATTCAGCGCGGGCTGGATCTGCTGCGCGGCTGGGATGGCAACGAGCGTGCCGACAGCGCGCCCGCCGCGC TGTTCGAGGTCTGGTTCAGCAAGCATCTGCGTACTGCGGTGGTGCGGGCCGCGCTCGCGCCGGAGGCTGCGCGGCTGATT GGCGCGGGCGATCCGAGCCGCGTGATCGCCGTGCTGGAGCGGCCCGACAGCTGGATGAGCGTCGAGAAGCGCAACGACGT CATGCTGGCCTCGCTGAAGTCGGCCATGGTCGAAGTCGAGAAGAGGCTGGGCCCTGACCCGAAGACGTGGCAATGGGGCA AGCTTCACACGGCGGTGTTCGAGCATCCGCTGCACGCGATCCTCGACGCGCAGCAGCGCCAGCAGTTCGACGTGAATGCG GGGCCGATCGGCGGGTCATCGTTCACGCCGATGAATACGAGCTATCGCAATAACGACTTCCAGCTCACTGCAGGCGCGTC GTTCCGCATGGTGGTCGATGTCGGCAACTGGGATGCGTCGCGCGTGGTGAACACACCGGGCCAGTCGGGCAACCCGGCCA ATCCGCACTATCGCGATCTGGCGCCGCTGTGGGCGAAGGGCGAGTATTTCCCGCTGCTGTACTCGCGCAAGGCGGTGGAG AAGGAAAGCAAGGAGAGGCTGGAACTGGTGCCGCAGTGA
Upstream 100 bases:
>100_bases GTGGAAGCCATCGCGGACGACATGATTTGCGGCGGCGCAGCGTCGCCGGCAGCGACACATTCCGCTATCCTCGCAGGTCT GCATCCGACGAGGAGATCCG
Downstream 100 bases:
>100_bases TGGATCGTGGCACGGCCTGCCGGGTGGCCTGACTGGGGCCGAGTCCTGCATTGCAGGGCTCGGCCCTTTTCTCTTTGCGG GGCCTTACCTGATCAGGCCA
Product: penicillin amidase (peptidase S45)
Products: NA
Alternate protein names: Cephalosporin acylase II; Penicillin acylase II; Penicillin amidase II; Penicillin acylase II small subunit; Penicillin acylase II large subunit [H]
Number of amino acids: Translated: 812; Mature: 812
Protein sequence:
>812_residues MFRPTRSLRRTAVAAAWLAAASTAPALAAGPDKFAVPGLEKPATVLIDRWGVPHIYANTLYDAFFVQGFVAARDRLFQID LWRKRGLGEMAKDFGPAYVEGDRMARAVLFRGDMYREWLAYGSDAKRVAEAFVAGVNAYIAQTEAHPELLPPEFKLINYK PSRWRAEDIVRIRHHGLTLNLTGEIDRAQIFCYAKPNAVRADWVRRELDPQIEPKVPDGLDPCTVPAAELRKAYELATAA PRFPKDVWQSGTRAENIPVDQLYATVDASSDTARSLGSNNWVIAGSRTATGRPILANDPHRAHGAPSLRYISHLNAPGLS VIGAGEPFLPGISIGHNGTIAFGLTRFYMDQEDLYAYETDPAQPHEYKYKGRWEPMETVTEQISVRGEATPRKVTIDFTR HGPVLYADSKNNRAWALRAAWLDLGMAPYFGSMDYMRAQNWDQFRAAMNRWGAPGENQVYADRFGNIGWLPGGLTVNRPN WDGLMPVPGDGRYEWAGYRNMDELPWAFNPAPGFVVTANENTIPPNHPAAKLGVGYEWAESSRARRLRALVSANPRSSVE DSMAWQNDTVSLPAQRMVTLLKPLASSDAQIQRGLDLLRGWDGNERADSAPAALFEVWFSKHLRTAVVRAALAPEAARLI GAGDPSRVIAVLERPDSWMSVEKRNDVMLASLKSAMVEVEKRLGPDPKTWQWGKLHTAVFEHPLHAILDAQQRQQFDVNA GPIGGSSFTPMNTSYRNNDFQLTAGASFRMVVDVGNWDASRVVNTPGQSGNPANPHYRDLAPLWAKGEYFPLLYSRKAVE KESKERLELVPQ
Sequences:
>Translated_812_residues MFRPTRSLRRTAVAAAWLAAASTAPALAAGPDKFAVPGLEKPATVLIDRWGVPHIYANTLYDAFFVQGFVAARDRLFQID LWRKRGLGEMAKDFGPAYVEGDRMARAVLFRGDMYREWLAYGSDAKRVAEAFVAGVNAYIAQTEAHPELLPPEFKLINYK PSRWRAEDIVRIRHHGLTLNLTGEIDRAQIFCYAKPNAVRADWVRRELDPQIEPKVPDGLDPCTVPAAELRKAYELATAA PRFPKDVWQSGTRAENIPVDQLYATVDASSDTARSLGSNNWVIAGSRTATGRPILANDPHRAHGAPSLRYISHLNAPGLS VIGAGEPFLPGISIGHNGTIAFGLTRFYMDQEDLYAYETDPAQPHEYKYKGRWEPMETVTEQISVRGEATPRKVTIDFTR HGPVLYADSKNNRAWALRAAWLDLGMAPYFGSMDYMRAQNWDQFRAAMNRWGAPGENQVYADRFGNIGWLPGGLTVNRPN WDGLMPVPGDGRYEWAGYRNMDELPWAFNPAPGFVVTANENTIPPNHPAAKLGVGYEWAESSRARRLRALVSANPRSSVE DSMAWQNDTVSLPAQRMVTLLKPLASSDAQIQRGLDLLRGWDGNERADSAPAALFEVWFSKHLRTAVVRAALAPEAARLI GAGDPSRVIAVLERPDSWMSVEKRNDVMLASLKSAMVEVEKRLGPDPKTWQWGKLHTAVFEHPLHAILDAQQRQQFDVNA GPIGGSSFTPMNTSYRNNDFQLTAGASFRMVVDVGNWDASRVVNTPGQSGNPANPHYRDLAPLWAKGEYFPLLYSRKAVE KESKERLELVPQ >Mature_812_residues MFRPTRSLRRTAVAAAWLAAASTAPALAAGPDKFAVPGLEKPATVLIDRWGVPHIYANTLYDAFFVQGFVAARDRLFQID LWRKRGLGEMAKDFGPAYVEGDRMARAVLFRGDMYREWLAYGSDAKRVAEAFVAGVNAYIAQTEAHPELLPPEFKLINYK PSRWRAEDIVRIRHHGLTLNLTGEIDRAQIFCYAKPNAVRADWVRRELDPQIEPKVPDGLDPCTVPAAELRKAYELATAA PRFPKDVWQSGTRAENIPVDQLYATVDASSDTARSLGSNNWVIAGSRTATGRPILANDPHRAHGAPSLRYISHLNAPGLS VIGAGEPFLPGISIGHNGTIAFGLTRFYMDQEDLYAYETDPAQPHEYKYKGRWEPMETVTEQISVRGEATPRKVTIDFTR HGPVLYADSKNNRAWALRAAWLDLGMAPYFGSMDYMRAQNWDQFRAAMNRWGAPGENQVYADRFGNIGWLPGGLTVNRPN WDGLMPVPGDGRYEWAGYRNMDELPWAFNPAPGFVVTANENTIPPNHPAAKLGVGYEWAESSRARRLRALVSANPRSSVE DSMAWQNDTVSLPAQRMVTLLKPLASSDAQIQRGLDLLRGWDGNERADSAPAALFEVWFSKHLRTAVVRAALAPEAARLI GAGDPSRVIAVLERPDSWMSVEKRNDVMLASLKSAMVEVEKRLGPDPKTWQWGKLHTAVFEHPLHAILDAQQRQQFDVNA GPIGGSSFTPMNTSYRNNDFQLTAGASFRMVVDVGNWDASRVVNTPGQSGNPANPHYRDLAPLWAKGEYFPLLYSRKAVE KESKERLELVPQ
Specific function: Unknown
COG id: COG2366
COG function: function code R; Protein related to penicillin acylase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase S45 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014395 - InterPro: IPR002692 [H]
Pfam domain/function: PF01804 Penicil_amidase [H]
EC number: =3.5.1.11 [H]
Molecular weight: Translated: 90380; Mature: 90380
Theoretical pI: Translated: 8.71; Mature: 8.71
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFRPTRSLRRTAVAAAWLAAASTAPALAAGPDKFAVPGLEKPATVLIDRWGVPHIYANTL CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH YDAFFVQGFVAARDRLFQIDLWRKRGLGEMAKDFGPAYVEGDRMARAVLFRGDMYREWLA HHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCEECCHHHHHHHHHCCHHHHHHHH YGSDAKRVAEAFVAGVNAYIAQTEAHPELLPPEFKLINYKPSRWRAEDIVRIRHHGLTLN CCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEE LTGEIDRAQIFCYAKPNAVRADWVRRELDPQIEPKVPDGLDPCTVPAAELRKAYELATAA EECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC PRFPKDVWQSGTRAENIPVDQLYATVDASSDTARSLGSNNWVIAGSRTATGRPILANDPH CCCCHHHHCCCCCCCCCCHHHHEEEECCCCHHHHHCCCCCEEEECCCCCCCCCEECCCCC RAHGAPSLRYISHLNAPGLSVIGAGEPFLPGISIGHNGTIAFGLTRFYMDQEDLYAYETD CCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEEEHHHHHCCHHHCEEEECC PAQPHEYKYKGRWEPMETVTEQISVRGEATPRKVTIDFTRHGPVLYADSKNNRAWALRAA CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEH WLDLGMAPYFGSMDYMRAQNWDQFRAAMNRWGAPGENQVYADRFGNIGWLPGGLTVNRPN HHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEECCCC WDGLMPVPGDGRYEWAGYRNMDELPWAFNPAPGFVVTANENTIPPNHPAAKLGVGYEWAE CCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCCHHHH SSRARRLRALVSANPRSSVEDSMAWQNDTVSLPAQRMVTLLKPLASSDAQIQRGLDLLRG HHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC WDGNERADSAPAALFEVWFSKHLRTAVVRAALAPEAARLIGAGDPSRVIAVLERPDSWMS CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHEEEEEECCCCCCC VEKRNDVMLASLKSAMVEVEKRLGPDPKTWQWGKLHTAVFEHPLHAILDAQQRQQFDVNA HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCC GPIGGSSFTPMNTSYRNNDFQLTAGASFRMVVDVGNWDASRVVNTPGQSGNPANPHYRDL CCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHC APLWAKGEYFPLLYSRKAVEKESKERLELVPQ CCHHCCCCEEEHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MFRPTRSLRRTAVAAAWLAAASTAPALAAGPDKFAVPGLEKPATVLIDRWGVPHIYANTL CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHH YDAFFVQGFVAARDRLFQIDLWRKRGLGEMAKDFGPAYVEGDRMARAVLFRGDMYREWLA HHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCEECCHHHHHHHHHCCHHHHHHHH YGSDAKRVAEAFVAGVNAYIAQTEAHPELLPPEFKLINYKPSRWRAEDIVRIRHHGLTLN CCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEE LTGEIDRAQIFCYAKPNAVRADWVRRELDPQIEPKVPDGLDPCTVPAAELRKAYELATAA EECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC PRFPKDVWQSGTRAENIPVDQLYATVDASSDTARSLGSNNWVIAGSRTATGRPILANDPH CCCCHHHHCCCCCCCCCCHHHHEEEECCCCHHHHHCCCCCEEEECCCCCCCCCEECCCCC RAHGAPSLRYISHLNAPGLSVIGAGEPFLPGISIGHNGTIAFGLTRFYMDQEDLYAYETD CCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEEEHHHHHCCHHHCEEEECC PAQPHEYKYKGRWEPMETVTEQISVRGEATPRKVTIDFTRHGPVLYADSKNNRAWALRAA CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEH WLDLGMAPYFGSMDYMRAQNWDQFRAAMNRWGAPGENQVYADRFGNIGWLPGGLTVNRPN HHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEECCCC WDGLMPVPGDGRYEWAGYRNMDELPWAFNPAPGFVVTANENTIPPNHPAAKLGVGYEWAE CCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHCCCCCHHHH SSRARRLRALVSANPRSSVEDSMAWQNDTVSLPAQRMVTLLKPLASSDAQIQRGLDLLRG HHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC WDGNERADSAPAALFEVWFSKHLRTAVVRAALAPEAARLIGAGDPSRVIAVLERPDSWMS CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHEEEEEECCCCCCC VEKRNDVMLASLKSAMVEVEKRLGPDPKTWQWGKLHTAVFEHPLHAILDAQQRQQFDVNA HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHCCCCC GPIGGSSFTPMNTSYRNNDFQLTAGASFRMVVDVGNWDASRVVNTPGQSGNPANPHYRDL CCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCHHHC APLWAKGEYFPLLYSRKAVEKESKERLELVPQ CCHHCCCCEEEHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3680178 [H]