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The map label for this gene is emrB [H]

Identifier: 94314510

GI number: 94314510

Start: 2344887

End: 2346461

Strand: Reverse

Name: emrB [H]

Synonym: Rmet_5591

Alternate gene names: 94314510

Gene position: 2346461-2344887 (Counterclockwise)

Preceding gene: 94314511

Following gene: 94314509

Centisome position: 90.95

GC content: 64.76

Gene sequence:

>1575_bases
ATGGCTGAAGCGGCGGCAATCGCGCGGGAGCCGAACGCTTCCGCGGCGGACTGGATTGCCGTGGCCGCCGGTTCCCTCGG
GGCGCTCCTGGCCACGCTTGATATCTCCATCACGAATTCGGCGCTGCCGCAGATCCAGGGCCAGATCGGGGCGGCCGGTA
CCGAGGGTACCTGGATCTCTACCGGCTATCTGATGGCCGAGATCGTGATCATTCCGCTCACGGCATGGCTGACGCGCGTG
TTCGGGCTGCGCCGCTTCCTGATGTCGAACGCGCTGCTGTTCACGGCGTTCTCGATGATGTGCGGCATGTCGCATAGCCT
GACCCAAATGGTGGTCGGCCGTATCGGCCAGGGGTTTGCCGGTGGCGCGATGATTCCGACTGCGCAGACCATCGTGCGGA
CGCGGCTGCCCCGGCACCAGATGCCGATCGGGATGTCGATGTTCGGCCTGATCGTGTTGCTCGGGCCCCTGCTCGGCCCG
GTAGTGGGCGGGTGGCTGGCCGAGAACGTCAACTGGCGCTGGTGCTTCTTCCTGAATCTGCCGATCACGGCGGTGCTGGT
ACCTCTGCTGTACTTCGGGCTGAAATCCGAGCGCGCGGATTTCAACGCATTCTTCAAGGCCGACTGGCTCGGCATCGTTG
GCCTTGCGGTGGGTCTCAGCTCCCTGACCGTGGTGCTCGAGGAAGGCCAGCGCGAGCAATGGTTCGAGTCACACCTGATT
GTCTATCTGTCGCTGCTGGCGGCGGGCGGCCTGGCACTGATGCTTGTCGGGCAGCTTACGGCGAAGCAGCCGATCGTGCG
TCTGCGCCTGCTGCTGAACAAGAACTACGCAAGCGTGATCCTGATCGTGACCACGGTGGGCGCCGGGGTGTACGGCGTGT
CCTACCTGCTGCCGCAGTTCCTGGCGACGATCGCCGGCTACAACGCCGAGCAGGCGGGCGGCATCATGCTGCTGTCGGGC
CTGCCCGCGTTTCTGCTGATGCCCGTGCTGCCGCGTATGCTCGGCAAGTTCGACAGCCGCTGGCTGGTCATCTCGGGGCT
GGTGTGCTTCTTCGCCAGTTGCATGCTCGATATCGGCCTGACCGTCGACAGCGTTGGCCATGACTTCTACGCATCGCAGA
TCCTGCGGGGCTTCGGCCAGATCCTGGCGATGATGCCGCTGAATCAGGCATCGATGGCCGCCGTGGCCACGCACGAAGCG
GGCGATGCCGCGGGCCTGTACAACATGGCGCGCAACCTTGGCGGTTCGATTGGCCTCGCGCTGCTCGGCACGCTGATCGA
CCGGCGCAATGCCTATCACGACTCGGTGATCCGCGAATCGGTGACCGCTAACAGCGCGATCGGTCAGGAGCACATGACGT
CGAGCATCGCGAGTTTCATGGCCCAGGGCGGCGACTATGCTCATGCCCAGTTGCAGGCGATTGCCCAGCTGAGTGGCCAG
ATTGCCCGCCAGGCGGCTGTGATGACGTTCTCCGAAACCTTCTTCGCGCTCGGCGTGGCCCTGCTGTTCTGTGTGCCGCT
GGCCCTGCTGCTGAAACCGCCCCGTCCGGGCGCACCTATTTCCACCGGTCACTGA

Upstream 100 bases:

>100_bases
GAAGCGCGCGCGATCCTGGTGCCGGGCCTGTCGGTAGTCGTCGACATCGACACGCGCGAGCTCCATCCCGCCAGGACCCA
GGCCGACATCAAGGCCACCC

Downstream 100 bases:

>100_bases
TCATGTCTGTTTCCTTTCGTGCCACCTTGCTGACGCTTGCGGCGGCGATGTTCGCCGCGGGCTGCACCGTTGGCCCCGAC
TATCGCGGCGCGCCGCAGGT

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAEAAAIAREPNASAADWIAVAAGSLGALLATLDISITNSALPQIQGQIGAAGTEGTWISTGYLMAEIVIIPLTAWLTRV
FGLRRFLMSNALLFTAFSMMCGMSHSLTQMVVGRIGQGFAGGAMIPTAQTIVRTRLPRHQMPIGMSMFGLIVLLGPLLGP
VVGGWLAENVNWRWCFFLNLPITAVLVPLLYFGLKSERADFNAFFKADWLGIVGLAVGLSSLTVVLEEGQREQWFESHLI
VYLSLLAAGGLALMLVGQLTAKQPIVRLRLLLNKNYASVILIVTTVGAGVYGVSYLLPQFLATIAGYNAEQAGGIMLLSG
LPAFLLMPVLPRMLGKFDSRWLVISGLVCFFASCMLDIGLTVDSVGHDFYASQILRGFGQILAMMPLNQASMAAVATHEA
GDAAGLYNMARNLGGSIGLALLGTLIDRRNAYHDSVIRESVTANSAIGQEHMTSSIASFMAQGGDYAHAQLQAIAQLSGQ
IARQAAVMTFSETFFALGVALLFCVPLALLLKPPRPGAPISTGH

Sequences:

>Translated_524_residues
MAEAAAIAREPNASAADWIAVAAGSLGALLATLDISITNSALPQIQGQIGAAGTEGTWISTGYLMAEIVIIPLTAWLTRV
FGLRRFLMSNALLFTAFSMMCGMSHSLTQMVVGRIGQGFAGGAMIPTAQTIVRTRLPRHQMPIGMSMFGLIVLLGPLLGP
VVGGWLAENVNWRWCFFLNLPITAVLVPLLYFGLKSERADFNAFFKADWLGIVGLAVGLSSLTVVLEEGQREQWFESHLI
VYLSLLAAGGLALMLVGQLTAKQPIVRLRLLLNKNYASVILIVTTVGAGVYGVSYLLPQFLATIAGYNAEQAGGIMLLSG
LPAFLLMPVLPRMLGKFDSRWLVISGLVCFFASCMLDIGLTVDSVGHDFYASQILRGFGQILAMMPLNQASMAAVATHEA
GDAAGLYNMARNLGGSIGLALLGTLIDRRNAYHDSVIRESVTANSAIGQEHMTSSIASFMAQGGDYAHAQLQAIAQLSGQ
IARQAAVMTFSETFFALGVALLFCVPLALLLKPPRPGAPISTGH
>Mature_523_residues
AEAAAIAREPNASAADWIAVAAGSLGALLATLDISITNSALPQIQGQIGAAGTEGTWISTGYLMAEIVIIPLTAWLTRVF
GLRRFLMSNALLFTAFSMMCGMSHSLTQMVVGRIGQGFAGGAMIPTAQTIVRTRLPRHQMPIGMSMFGLIVLLGPLLGPV
VGGWLAENVNWRWCFFLNLPITAVLVPLLYFGLKSERADFNAFFKADWLGIVGLAVGLSSLTVVLEEGQREQWFESHLIV
YLSLLAAGGLALMLVGQLTAKQPIVRLRLLLNKNYASVILIVTTVGAGVYGVSYLLPQFLATIAGYNAEQAGGIMLLSGL
PAFLLMPVLPRMLGKFDSRWLVISGLVCFFASCMLDIGLTVDSVGHDFYASQILRGFGQILAMMPLNQASMAAVATHEAG
DAAGLYNMARNLGGSIGLALLGTLIDRRNAYHDSVIRESVTANSAIGQEHMTSSIASFMAQGGDYAHAQLQAIAQLSGQI
ARQAAVMTFSETFFALGVALLFCVPLALLLKPPRPGAPISTGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=509, Percent_Identity=29.2730844793713, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1788710, Length=502, Percent_Identity=26.4940239043825, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1790195, Length=342, Percent_Identity=28.6549707602339, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=24.5862884160757, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788392, Length=428, Percent_Identity=23.8317757009346, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=200, Percent_Identity=24.5, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=136, Percent_Identity=30.8823529411765, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=24.1610738255034, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55691; Mature: 55560

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAAAIAREPNASAADWIAVAAGSLGALLATLDISITNSALPQIQGQIGAAGTEGTWIS
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEE
TGYLMAEIVIIPLTAWLTRVFGLRRFLMSNALLFTAFSMMCGMSHSLTQMVVGRIGQGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
GGAMIPTAQTIVRTRLPRHQMPIGMSMFGLIVLLGPLLGPVVGGWLAENVNWRWCFFLNL
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
PITAVLVPLLYFGLKSERADFNAFFKADWLGIVGLAVGLSSLTVVLEEGQREQWFESHLI
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VYLSLLAAGGLALMLVGQLTAKQPIVRLRLLLNKNYASVILIVTTVGAGVYGVSYLLPQF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHCCHHHHHHHHHHHH
LATIAGYNAEQAGGIMLLSGLPAFLLMPVLPRMLGKFDSRWLVISGLVCFFASCMLDIGL
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
TVDSVGHDFYASQILRGFGQILAMMPLNQASMAAVATHEAGDAAGLYNMARNLGGSIGLA
CHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LLGTLIDRRNAYHDSVIRESVTANSAIGQEHMTSSIASFMAQGGDYAHAQLQAIAQLSGQ
HHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IARQAAVMTFSETFFALGVALLFCVPLALLLKPPRPGAPISTGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEAAAIAREPNASAADWIAVAAGSLGALLATLDISITNSALPQIQGQIGAAGTEGTWIS
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEE
TGYLMAEIVIIPLTAWLTRVFGLRRFLMSNALLFTAFSMMCGMSHSLTQMVVGRIGQGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
GGAMIPTAQTIVRTRLPRHQMPIGMSMFGLIVLLGPLLGPVVGGWLAENVNWRWCFFLNL
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
PITAVLVPLLYFGLKSERADFNAFFKADWLGIVGLAVGLSSLTVVLEEGQREQWFESHLI
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VYLSLLAAGGLALMLVGQLTAKQPIVRLRLLLNKNYASVILIVTTVGAGVYGVSYLLPQF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHCCHHHHHHHHHHHH
LATIAGYNAEQAGGIMLLSGLPAFLLMPVLPRMLGKFDSRWLVISGLVCFFASCMLDIGL
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
TVDSVGHDFYASQILRGFGQILAMMPLNQASMAAVATHEAGDAAGLYNMARNLGGSIGLA
CHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
LLGTLIDRRNAYHDSVIRESVTANSAIGQEHMTSSIASFMAQGGDYAHAQLQAIAQLSGQ
HHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IARQAAVMTFSETFFALGVALLFCVPLALLLKPPRPGAPISTGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]