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The map label for this gene is emrA [H]

Identifier: 94314511

GI number: 94314511

Start: 2346454

End: 2347599

Strand: Reverse

Name: emrA [H]

Synonym: Rmet_5592

Alternate gene names: 94314511

Gene position: 2347599-2346454 (Counterclockwise)

Preceding gene: 94314516

Following gene: 94314510

Centisome position: 90.99

GC content: 66.49

Gene sequence:

>1146_bases
ATGTCCGCCACCCACTCCGCCGCAACGGCGTCGCCAGCCGGCGTCGCGCCAGAATCCGCCGTTCCCGATCGCACTTCACG
CCGCAAGCAGATCCTGCTCGGCGCGGGTTTGCTGCTTCTCGCGCTTGCCCTGGCGTACGGTGTGCGCTGGTGGCTCTATG
GCCGCTTCATCGAAAGCACCGATGACGCCTATATACAGGCCGATAGCGTGACGGTGGCACCCAAGGTCAACGGCTACGTG
GCCGAGGTCTACGTGCGTGACAACCAGCAGATCCAGGCGGGTCAGGAACTGGTGCGGCTCGACAATCGTCAGTACCAGGC
AGCCTATGATGAGTCGCAGGCGACCGTGGCTTCGCGGCAGGCCGACGTGACGCGCGCGGAAGCCGATCTCGCGCAGCAGC
AGGCGAGGATCGCGCAGGCTGCCGCGGAAGTGGATGGCGCGCGAGCCAATTCGCGCTATGCGGCATCGCAGGTGGAACGC
TATGCGCCACTGGTGAAATCGGGCGCCGAAACGGAAGAGCGCACGGCCGAGCTGCGCAATGCGCAGACCCGCGCATCCAC
CACGCTGGCTGCCAACGAGGCTGCCTTGCGGGTGGAACAAAGCCAGACGGTGACGCTGAAGGCGCGGCTCGAGCAGGCGC
ATGCGCAGCTCGACGTGGCCCAGGCCAGCGCGCGTCAGGCAAAGCTCAATCTCGATGACACCGTGGTGCGATCCACCACG
GCCGGGCGTGTTGCCGACAAGGGTGTGCGCGTCGGCCAGTTCGCCCAGCCCGGCATGCGTCTGCTGACCGTGGTGCCGGT
ACAGGACCTCTATCTGACCGCCAATTTCAAGGAAACGCAGGTGGGCCGCATGCGCCCAGGCCAGCCGGTGACGCTCCACG
TCGACGCACTACCCGGTGAGGAACTGCATGGTGTGGTCGATAGCCTGTCGCCCGGTACCGGCTCGCAGTTTGCCCTGCTG
CCCGCACAGAATGCCACGGGCAACTTCACCAAGATCGTCCAGCGCGTGCCGGTGCGGATTCATGTGGACGTGCCGACGGA
AGCGCGCGCGATCCTGGTGCCGGGCCTGTCGGTAGTCGTCGACATCGACACGCGCGAGCTCCATCCCGCCAGGACCCAGG
CCGACATCAAGGCCACCCATGGCTGA

Upstream 100 bases:

>100_bases
GTCCTTGTCGCTGAATTCAGACAGAAATATACCAGTTGACAGATTTTTATCAAGTGGAATAATTGCTGAGCCATTCCGAC
TTCGAAGTCCCCGCGTCCCC

Downstream 100 bases:

>100_bases
AGCGGCGGCAATCGCGCGGGAGCCGAACGCTTCCGCGGCGGACTGGATTGCCGTGGCCGCCGGTTCCCTCGGGGCGCTCC
TGGCCACGCTTGATATCTCC

Product: multidrug resistance protein (hlyD family), emrA-like secretion

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSATHSAATASPAGVAPESAVPDRTSRRKQILLGAGLLLLALALAYGVRWWLYGRFIESTDDAYIQADSVTVAPKVNGYV
AEVYVRDNQQIQAGQELVRLDNRQYQAAYDESQATVASRQADVTRAEADLAQQQARIAQAAAEVDGARANSRYAASQVER
YAPLVKSGAETEERTAELRNAQTRASTTLAANEAALRVEQSQTVTLKARLEQAHAQLDVAQASARQAKLNLDDTVVRSTT
AGRVADKGVRVGQFAQPGMRLLTVVPVQDLYLTANFKETQVGRMRPGQPVTLHVDALPGEELHGVVDSLSPGTGSQFALL
PAQNATGNFTKIVQRVPVRIHVDVPTEARAILVPGLSVVVDIDTRELHPARTQADIKATHG

Sequences:

>Translated_381_residues
MSATHSAATASPAGVAPESAVPDRTSRRKQILLGAGLLLLALALAYGVRWWLYGRFIESTDDAYIQADSVTVAPKVNGYV
AEVYVRDNQQIQAGQELVRLDNRQYQAAYDESQATVASRQADVTRAEADLAQQQARIAQAAAEVDGARANSRYAASQVER
YAPLVKSGAETEERTAELRNAQTRASTTLAANEAALRVEQSQTVTLKARLEQAHAQLDVAQASARQAKLNLDDTVVRSTT
AGRVADKGVRVGQFAQPGMRLLTVVPVQDLYLTANFKETQVGRMRPGQPVTLHVDALPGEELHGVVDSLSPGTGSQFALL
PAQNATGNFTKIVQRVPVRIHVDVPTEARAILVPGLSVVVDIDTRELHPARTQADIKATHG
>Mature_380_residues
SATHSAATASPAGVAPESAVPDRTSRRKQILLGAGLLLLALALAYGVRWWLYGRFIESTDDAYIQADSVTVAPKVNGYVA
EVYVRDNQQIQAGQELVRLDNRQYQAAYDESQATVASRQADVTRAEADLAQQQARIAQAAAEVDGARANSRYAASQVERY
APLVKSGAETEERTAELRNAQTRASTTLAANEAALRVEQSQTVTLKARLEQAHAQLDVAQASARQAKLNLDDTVVRSTTA
GRVADKGVRVGQFAQPGMRLLTVVPVQDLYLTANFKETQVGRMRPGQPVTLHVDALPGEELHGVVDSLSPGTGSQFALLP
AQNATGNFTKIVQRVPVRIHVDVPTEARAILVPGLSVVVDIDTRELHPARTQADIKATHG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=343, Percent_Identity=34.402332361516, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788711, Length=345, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1790519, Length=292, Percent_Identity=31.1643835616438, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081951, Length=303, Percent_Identity=26.7326732673267, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790024, Length=251, Percent_Identity=28.2868525896414, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789637, Length=317, Percent_Identity=28.0757097791798, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790012, Length=249, Percent_Identity=27.710843373494, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789900, Length=263, Percent_Identity=25.8555133079848, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40952; Mature: 40821

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATHSAATASPAGVAPESAVPDRTSRRKQILLGAGLLLLALALAYGVRWWLYGRFIEST
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDAYIQADSVTVAPKVNGYVAEVYVRDNQQIQAGQELVRLDNRQYQAAYDESQATVASRQ
CCCEEEECCEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHH
ADVTRAEADLAQQQARIAQAAAEVDGARANSRYAASQVERYAPLVKSGAETEERTAELRN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
AQTRASTTLAANEAALRVEQSQTVTLKARLEQAHAQLDVAQASARQAKLNLDDTVVRSTT
CHHHHHHEEECCCHHEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCC
AGRVADKGVRVGQFAQPGMRLLTVVPVQDLYLTANFKETQVGRMRPGQPVTLHVDALPGE
CCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCH
ELHGVVDSLSPGTGSQFALLPAQNATGNFTKIVQRVPVRIHVDVPTEARAILVPGLSVVV
HHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCEEEE
DIDTRELHPARTQADIKATHG
EECCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SATHSAATASPAGVAPESAVPDRTSRRKQILLGAGLLLLALALAYGVRWWLYGRFIEST
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDAYIQADSVTVAPKVNGYVAEVYVRDNQQIQAGQELVRLDNRQYQAAYDESQATVASRQ
CCCEEEECCEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHH
ADVTRAEADLAQQQARIAQAAAEVDGARANSRYAASQVERYAPLVKSGAETEERTAELRN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
AQTRASTTLAANEAALRVEQSQTVTLKARLEQAHAQLDVAQASARQAKLNLDDTVVRSTT
CHHHHHHEEECCCHHEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCC
AGRVADKGVRVGQFAQPGMRLLTVVPVQDLYLTANFKETQVGRMRPGQPVTLHVDALPGE
CCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCH
ELHGVVDSLSPGTGSQFALLPAQNATGNFTKIVQRVPVRIHVDVPTEARAILVPGLSVVV
HHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCEEEE
DIDTRELHPARTQADIKATHG
EECCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]