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The map label for this gene is gcvA [H]

Identifier: 94314343

GI number: 94314343

Start: 2171531

End: 2172445

Strand: Direct

Name: gcvA [H]

Synonym: Rmet_5424

Alternate gene names: 94314343

Gene position: 2171531-2172445 (Clockwise)

Preceding gene: 94314338

Following gene: 94314344

Centisome position: 84.17

GC content: 61.42

Gene sequence:

>915_bases
GTGATGAAGATTCCTCCGCTCAACCCATTGAAGGTCTTCGAGGTCGTGGCCAGAGCCAAGAACCTCACGACCGCCGCCAA
CGAACTTCGGGTCAGTCAATCGGCAGTGAGCCGTCAGATCGCGGTGCTGGAGGAATACCTGGGCGTCCAGCTCTTCACCC
GCGAACGCATTGGCGTGAAACTGACCGAGGTTGGTGAGGCCTACGCGCGGCGCATCGGCCCGGCGTTCGAGGAAATCTCG
GAGGCCACCAAGTTCGTCACCCAGAAGTACTCCGACAACGTCATTCGCCTGCGCACCTACACCACGCTGACAGCGCGATG
GCTGATTCCCAGGTTGCCGCATTTCAAGCTGCAGTACCCCGACGTCGAAGTGCTGATTACCAACAGCAACGCGCCGCTCG
ATTTCGGCACAGAGCAATGCGACCTGGCGATCGTCCTCGGTGATGGCAACTGGCCAAACGCCGAGGCAACGCTGCTGCTC
GAGGATGTCATCGAACCCGTCTGCTCGCCCGCGTTCCTGGAAGCCTTCCATGGCAGCACGGAGCAGTTGCTGGAGTCGCT
GCAAAGCAAGCGCCTGCTGGTTTCCAAGTACCGCAAGAATGACTGGCCGACGTGGCTCGCGCACGTCGGCATGGGTCACG
TGTTCGACAAGGCCGAAAAGATGGTCTTCAGCAGTTCCATCCTCACGTGGCAGGCGGCCACGGACAACCTTGGCATCGCA
ATCGGCCAGCAGCACATGCTGGACCCCGACCTCAAGGCTGGACGGCTGGTCTGCCCGTTTGCAAGGCCGCTGCGTACCGG
GCGGGACCACTATATCGTGACGCCGTCGGTACAGCGTTACTCGGGCAAGATCGCGGCCTTCAAGGAATGGCTGGTGCGTG
AGGCCCGGGAAAGCAAGCCCATGACACCAGGCTGA

Upstream 100 bases:

>100_bases
TCGCAGGGCAGATCACAGCGCCCCTGAAACGTCCAGACGCACCTACAGCCGGACCATGCGTCGTCCGGCAAGCACACGAG
AGACTGGGAAGACCAGCGAC

Downstream 100 bases:

>100_bases
TGCTTTATGGCGCCAAGGCCGGCGGATTCGGCCCCTTACCCGCGCCGAATCATGCGATACCCGACCGGGTAATCGTTCCT
CTCCAGAAACTCGCCGCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVKLTEVGEAYARRIGPAFEEIS
EATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYPDVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLL
EDVIEPVCSPAFLEAFHGSTEQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIA
IGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKPMTPG

Sequences:

>Translated_304_residues
MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVKLTEVGEAYARRIGPAFEEIS
EATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYPDVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLL
EDVIEPVCSPAFLEAFHGSTEQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIA
IGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKPMTPG
>Mature_304_residues
MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVKLTEVGEAYARRIGPAFEEIS
EATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYPDVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLL
EDVIEPVCSPAFLEAFHGSTEQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIA
IGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKPMTPG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=34.5762711864407, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=28.6195286195286, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=29.2096219931272, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786401, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081978, Length=265, Percent_Identity=28.6792452830189, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=148, Percent_Identity=30.4054054054054, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=24.4147157190635, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=31.7567567567568, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=171, Percent_Identity=30.4093567251462, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34208; Mature: 34208

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCEE
LTEVGEAYARRIGPAFEEISEATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYP
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCCCEEECC
DVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLLEDVIEPVCSPAFLEAFHGST
CEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCH
EQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEE
IGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKP
ECCCCCCCCCCCCCCEECCCHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHCCC
MTPG
CCCC
>Mature Secondary Structure
MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCEE
LTEVGEAYARRIGPAFEEISEATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYP
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCCCEEECC
DVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLLEDVIEPVCSPAFLEAFHGST
CEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCH
EQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEE
IGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKP
ECCCCCCCCCCCCCCEECCCHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHCCC
MTPG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]