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The map label for this gene is hipO [H]

Identifier: 94314338

GI number: 94314338

Start: 2165250

End: 2166431

Strand: Direct

Name: hipO [H]

Synonym: Rmet_5419

Alternate gene names: 94314338

Gene position: 2165250-2166431 (Clockwise)

Preceding gene: 94314337

Following gene: 94314343

Centisome position: 83.92

GC content: 67.09

Gene sequence:

>1182_bases
ATGACGACCACACCCATTCCCGCCGCCATCTCGTCCGCAGCCAGCCGCTTTGCCGATCTGCGCCATCGCATCCATGCGCA
CCCGGAGCTCGGGGCCGACGTGCCGCGCACCGCCGCCATGGTGGCCGGTCTGCTGCGCGAATGGGGCTACGACGTCCATA
CCGGCATTGGCGGCCACGGCATCGTGGCCCAGCTCAATGTCGGCAACGGCCCGCGCCGCATCGGCATTCGGGCCGACATG
GATGCGCTCCCGCTGCAGGAACGCACCGGCTTGCCCTATGCCAGCGAAGTACCCGGCCGTATGCACGCCTGCGGGCATGA
CGGGCACACGGCCACGCTACTGGCCGCTGCCGAATATCTCGCGGCCACGCGCAACTTCGATGGCACGCTGAACCTGATCT
TCCAGCCTGACGAGGAAGGCATGACCGGCGCCCTGTCGATGATGGAGGATGGTCTCTTCGAACGCTTTCCGTGCGACGCG
ATCTACGCGTTCCACAACGCGCCGGGCCTGCCGGTCGGCATGGCCGTGGTCCAGTCTGGCGCGCAGGGCGGATCGTCGGA
CCGCGTGACCGTGACCTTCCGCGGCAAGGGCGGGCATGGCGCGATTCCGAATCGTGCCGCCGACCCCACGCTGGCGCTGG
CGCACACCGTGGTGGCCCTGCAAGGCATCATCGCGCGCAACGTGGCGCCGGTGGATGCGGGCGTGATCAGCGTGGGCCAG
ATCGAAGCGGGCACGACCTACAACATCATCCCGGAGACTGCCACGCTCAAGCTCAGTGTGCGTGCCACGTTGCCCGAGGT
CCGCAAGCTGCTGGAGGAACGCATCCGTGCCGTGGTCACGCATCAGGCCGCGTCGGCCGGTGTCGAGGCCGAGATCGAGT
ACCTGCATCTGGTGCCGGCCATCCGCAACACGCCGGCAGAAGCGGCGATCGCGCGCGAAGCCGTCGCCAGCGTGCTCGGC
AATGACAACGTCATCCAGGTCCCGCCCTCGACGCAGATGGGCAGCGAGGACTTTGCCTGGATGCTCGAGAAGGTGCCCGG
CTGCTATTTCGTGCTTGGCAACGGCACTGGCGAGTGGGTAGGTTGCTCGGTGCACAACGACGGCTACGACTTCAACGATG
CGCTAATTCCGCTCGGCGCCGCCTGCTGGGTAGCATTGGCAGAGGGCTATCTCAAGTCGTGA

Upstream 100 bases:

>100_bases
AGCTTTACTGCCCTTCTGCTGTTCCCCAGCCATCCGGGCCGCGATTGAGCGCCGCCTTGAAGATGCCCCGATTGCCCCAC
AACGCTCAGCCCCATTCATC

Downstream 100 bases:

>100_bases
TCGTCACGGCTTGCCCCTCTCGCAACCTGACCGCCAGACTCAGCAACACCGCCTCGAAGCCGCGCAACGTGGCGGTGACC
GGGCGGTCGACAGGTTGTTT

Product: peptidase, M20D subfamily

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHGIVAQLNVGNGPRRIGIRADM
DALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYLAATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDA
IYAFHNAPGLPVGMAVVQSGAQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQ
IEAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPAIRNTPAEAAIAREAVASVLG
NDNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWVGCSVHNDGYDFNDALIPLGAACWVALAEGYLKS

Sequences:

>Translated_393_residues
MTTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHGIVAQLNVGNGPRRIGIRADM
DALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYLAATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDA
IYAFHNAPGLPVGMAVVQSGAQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQ
IEAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPAIRNTPAEAAIAREAVASVLG
NDNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWVGCSVHNDGYDFNDALIPLGAACWVALAEGYLKS
>Mature_392_residues
TTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHGIVAQLNVGNGPRRIGIRADMD
ALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYLAATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDAI
YAFHNAPGLPVGMAVVQSGAQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQI
EAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPAIRNTPAEAAIAREAVASVLGN
DNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWVGCSVHNDGYDFNDALIPLGAACWVALAEGYLKS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=424, Percent_Identity=28.3018867924528, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41345; Mature: 41214

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCC
IVAQLNVGNGPRRIGIRADMDALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYL
EEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
AATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDAIYAFHNAPGLPVGMAVVQSG
HHHCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHCCCHHEEEECCCCCCCHHHHHHHCC
AQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQ
CCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECE
IEAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPA
ECCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHH
IRNTPAEAAIAREAVASVLGNDNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWV
HCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEE
GCSVHNDGYDFNDALIPLGAACWVALAEGYLKS
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCC
IVAQLNVGNGPRRIGIRADMDALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYL
EEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
AATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDAIYAFHNAPGLPVGMAVVQSG
HHHCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHCCCHHEEEECCCCCCCHHHHHHHCC
AQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQ
CCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECE
IEAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPA
ECCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHH
IRNTPAEAAIAREAVASVLGNDNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWV
HCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEE
GCSVHNDGYDFNDALIPLGAACWVALAEGYLKS
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]