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The map label for this gene is citH [H]

Identifier: 94314337

GI number: 94314337

Start: 2163893

End: 2165197

Strand: Direct

Name: citH [H]

Synonym: Rmet_5418

Alternate gene names: 94314337

Gene position: 2163893-2165197 (Clockwise)

Preceding gene: 94314336

Following gene: 94314338

Centisome position: 83.87

GC content: 67.13

Gene sequence:

>1305_bases
ATGAACGAAGCCACCGTTTCGCTGGGCAGCGATGCCGCAGCCCGCGTCGTCGCGCCCGCTTCCAGCACGCAGGGCCGCCG
CGCCGTCATCGCGGCTGCCATCGGCAATGGACTGGAGATCTACGACTTCACGGTCTACAGCTTCTTCGCTGCCACCATCG
GCCGGCTGTTCTTTCCCGCCAGCAGTCCGCTCGCCTCGTTGCTGCTTTCCTTTGCGACGTTCGGTGCTGGCTTCGTGATG
CGCCCGCTCGGCGCCGTGCTCATCGGCAACATGGCCGACCGGCGGGGCCGCAAGGCCGCGCTGACGCTGACGATCGGCCT
GATGACCGCCGGCACGGCGCTGCTGGCCTTCACGCCGTCCTACGCCAGCATCGGCGTGACCGCCACTGCGCTGGTGGTGG
TGGGCCGTCTGCTGCAGGGCCTGTCCGCTGGCGGCGAGGTTGGCGCCGCGTCCGCGCTGCTGATGGAGTCGACTGGCAAG
CAACGGCGCTGCTTCGCGGTGAGCTGGCAAGGCGCAAGCCAGGGCGCTGCGGCATTGCTGGGGGCGCTGACCGGCGCCGC
GGTGTCGGCGCTGCTGAGCCCCGAAGCACTCCAGTCATGGGGCTGGCGCGTGCCGTTCATGCTCGGCCTGCTGATCGCCC
CGGTCGGCATCTACATCCGCCGGCATCTCGAAGAGACCCAAACCGAAAACACCGCCCACGCCGGCCTCATGACGGTGGTG
CGCGAGCATCGCCGCACGCTGATTCTCGGCGTGCTGCTGATGACGAGCAGCACGTCGTCGATGTACATCATGGTGTTCTA
CATGCCGACCTATCTGGTCAACACCGTGCACATGCCGCCCGTGACCGCGTTCCTGGGCGCCTGCGTGGCAGGTCTGACGA
TGCTGGTCATGTCGCCGCTGTTCGGATTGCTCGCGGACCGGCTGCCGCGCCGCAAGCCGCTGCTGATCGCCAACAGCATT
GTCGCCATCAGCGTGATGGTGCCCAACTTCGCGCTGCTGTCTTCTGGCCCGGGGCTGCCATTGACGCTGGCCTGCATCGC
GTTATCGGTCACGTTGAGTTCCGTAGGCGGCGGCGCGGGATCGGCGCTGATGATGGAATCGCTGCCGCGCCATCACCGCG
CCACCGGTATGTCGATCATGTACAGCGTGGGCGTGACCGTTTTTGGCGGCTTCGCGCCGCTGGTGGTCACGTGGCTGATC
GCTACCACCGGTAGCCGCATGGCGCCGGTCGGATACATGCTGTGCGCGTCGCTGATCAGCTTTACTGCCCTTCTGCTGTT
CCCCAGCCATCCGGGCCGCGATTGA

Upstream 100 bases:

>100_bases
ATGGGTGCCAGGCACCTAAGATCGTCCGGTCGAACAGAAACCCATAAGAACACGGGCAGCCATTCAGCGACTGCCCGGAC
GGACCACCACGAGGAGCCTC

Downstream 100 bases:

>100_bases
GCGCCGCCTTGAAGATGCCCCGATTGCCCCACAACGCTCAGCCCCATTCATCATGACGACCACACCCATTCCCGCCGCCA
TCTCGTCCGCAGCCAGCCGC

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNEATVSLGSDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPASSPLASLLLSFATFGAGFVM
RPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPSYASIGVTATALVVVGRLLQGLSAGGEVGAASALLMESTGK
QRRCFAVSWQGASQGAAALLGALTGAAVSALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEETQTENTAHAGLMTVV
REHRRTLILGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPLFGLLADRLPRRKPLLIANSI
VAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAGSALMMESLPRHHRATGMSIMYSVGVTVFGGFAPLVVTWLI
ATTGSRMAPVGYMLCASLISFTALLLFPSHPGRD

Sequences:

>Translated_434_residues
MNEATVSLGSDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPASSPLASLLLSFATFGAGFVM
RPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPSYASIGVTATALVVVGRLLQGLSAGGEVGAASALLMESTGK
QRRCFAVSWQGASQGAAALLGALTGAAVSALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEETQTENTAHAGLMTVV
REHRRTLILGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPLFGLLADRLPRRKPLLIANSI
VAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAGSALMMESLPRHHRATGMSIMYSVGVTVFGGFAPLVVTWLI
ATTGSRMAPVGYMLCASLISFTALLLFPSHPGRD
>Mature_434_residues
MNEATVSLGSDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPASSPLASLLLSFATFGAGFVM
RPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPSYASIGVTATALVVVGRLLQGLSAGGEVGAASALLMESTGK
QRRCFAVSWQGASQGAAALLGALTGAAVSALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEETQTENTAHAGLMTVV
REHRRTLILGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPLFGLLADRLPRRKPLLIANSI
VAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAGSALMMESLPRHHRATGMSIMYSVGVTVFGGFAPLVVTWLI
ATTGSRMAPVGYMLCASLISFTALLLFPSHPGRD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=29.4536817102138, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788942, Length=404, Percent_Identity=33.9108910891089, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=30.7328605200946, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=31.4553990610329, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=27.6190476190476, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=412, Percent_Identity=24.2718446601942, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45117; Mature: 45117

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEATVSLGSDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPA
CCCCEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCC
SSPLASLLLSFATFGAGFVMRPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEHHHHCCEEEEECCC
YASIGVTATALVVVGRLLQGLSAGGEVGAASALLMESTGKQRRCFAVSWQGASQGAAALL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
GALTGAAVSALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEETQTENTAHAGLMTVV
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REHRRTLILGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPL
HHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FGLLADRLPRRKPLLIANSIVAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAG
HHHHHHHCCCCCCEEEECCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
SALMMESLPRHHRATGMSIMYSVGVTVFGGFAPLVVTWLIATTGSRMAPVGYMLCASLIS
HHHHHHHCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FTALLLFPSHPGRD
HHHHHHCCCCCCCC
>Mature Secondary Structure
MNEATVSLGSDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPA
CCCCEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCC
SSPLASLLLSFATFGAGFVMRPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCEEEEEEEEHHHHCCEEEEECCC
YASIGVTATALVVVGRLLQGLSAGGEVGAASALLMESTGKQRRCFAVSWQGASQGAAALL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
GALTGAAVSALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEETQTENTAHAGLMTVV
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REHRRTLILGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPL
HHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FGLLADRLPRRKPLLIANSIVAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAG
HHHHHHHCCCCCCEEEECCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
SALMMESLPRHHRATGMSIMYSVGVTVFGGFAPLVVTWLIATTGSRMAPVGYMLCASLIS
HHHHHHHCCHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FTALLLFPSHPGRD
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]