Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 94314033

GI number: 94314033

Start: 1807732

End: 1809120

Strand: Direct

Name: gdhA [H]

Synonym: Rmet_5114

Alternate gene names: 94314033

Gene position: 1807732-1809120 (Clockwise)

Preceding gene: 94314025

Following gene: 94314034

Centisome position: 70.06

GC content: 64.43

Gene sequence:

>1389_bases
GTGGCGCAGGCGCCGCACATCGCATCAAGAAAACTCAGAGACATCGTGACTTCTCGCACCCTCGATTCCTTCCTGGCCGG
GGTCTCCCGGCGTGACCCCAACCAGCCCGAATTCTTCCAGGCCGTGAAGGAGGTCATGATGACCTTGTGGCCGTTCGTCG
AACGCAACCCGCGCTATGGCGATCAGGCACTGCTGGAACGGCTGGTGGAACCGGAACGTGTGATCCAGTTCCGCGTGGCA
TGGACCGACGATCGCGGCCAGGTGCAGGTCAACCGCGCATTCCGCGTGCAGCACAGCTCCGCAATCGGCCCGTACAAGGG
TGGGATGCGCTTCCACCCGACCGTGAACCTGTCGGTACTGAAGTTCCTGGGCTTCGAGCAGACCTTCAAGAATGCGCTGA
CCACCCTGCCCATGGGCGGCGGCAAGGGCGGCTCGGACTTCGACCCAAAGGGCAAGACCGACGCTGAAGTCATGCGCTTC
TGCCAGGCGCTGATCACCGAGCTGTATCGTCACCTGGGACCGGACACCGACGTGCCGGCCGGTGACATCGGCGTGGGCGC
GCGGGAAGTCGGCTTCATGGCCGGCATGATGAAGAAGCTGTCGAACCAGTCCGCCTGCGTGTTCACTGGCAAGGGTCTGG
CCTTCGGCGGCAGCCTGATGCGTCCCGAGGCCACCGGCTACGGCACGGTGTACTTCGCGCAGGAGATGCTGCATCGTCGC
GGCCGCGAATTCGACGGTCTGCGTGTGCTGCTGTCGGGCTCGGGCAACGTGGCGCAATACGCCGCCGAAAAGGCGATCGA
GCTGGGTGCCAAGGTGCTGACGGTATCGGACTCCGGAGGCGTGCTGCACTTCCCCGAGGGCATGAATCGCGAGCAACTGG
ACGCGCTGATGACGTTCAAGAACGACACCCGTGGCCGCCTGTCCGACTTTGCCGAGCAGCATGGCCTGCGCTTCGAGGCC
GGCAAGACGCCGTGGCACGTGCCCGCCGACGTTGCTCTGCCGTGCGCCACCCAGAACGAACTGAACGGTCAGGATGCCGA
GCGCCTGCTGTCGAACGGCGTGTTCTGCGTAGCCGAAGGCGCCAACATGCCGTCGACGCTGGACGCCGTGGACCGCTTCG
TGGCCGCACGCATCCTGTATGCGCCGGGCAAGGCCAGCAACGCTGGCGGTGTGGCCACTTCGGGCCTGGAGATGTCGCAG
AACGCCATGCGCATGGCCTGGCATCACGCCGAGATCGACGAGAAGCTCCACGCCATCATGAAGGACATCCACGGCAACTG
CATCCACTACGGCCAGAAGGAAGACGGCTACATCAATTATGTGGAAGGCGCCAACATCGCCGGCTTCGTCAAGGTGGCGG
ACGCCATGCTGGCGCAGGGTGTGATCTGA

Upstream 100 bases:

>100_bases
GCCGCCTGACGCGGGGTCCCCATCGGGATTTCCCGGGTTTCCATGCCGCGGCGCCCACCAGACACGCCATTCGGAGCGAC
CCTTCCGCCATACCCACTGC

Downstream 100 bases:

>100_bases
TCCCGCGGCCGATCGGGCAGCTGGTCTAACCAGACACCCGGTTGCCAGCCCGGGAAGGAATGCGACGAGGCAAGCCTCGT
CGCATTTTCTTTTGCCCGCT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAQAPHIASRKLRDIVTSRTLDSFLAGVSRRDPNQPEFFQAVKEVMMTLWPFVERNPRYGDQALLERLVEPERVIQFRVA
WTDDRGQVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKTDAEVMRF
CQALITELYRHLGPDTDVPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRR
GREFDGLRVLLSGSGNVAQYAAEKAIELGAKVLTVSDSGGVLHFPEGMNREQLDALMTFKNDTRGRLSDFAEQHGLRFEA
GKTPWHVPADVALPCATQNELNGQDAERLLSNGVFCVAEGANMPSTLDAVDRFVAARILYAPGKASNAGGVATSGLEMSQ
NAMRMAWHHAEIDEKLHAIMKDIHGNCIHYGQKEDGYINYVEGANIAGFVKVADAMLAQGVI

Sequences:

>Translated_462_residues
MAQAPHIASRKLRDIVTSRTLDSFLAGVSRRDPNQPEFFQAVKEVMMTLWPFVERNPRYGDQALLERLVEPERVIQFRVA
WTDDRGQVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKTDAEVMRF
CQALITELYRHLGPDTDVPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRR
GREFDGLRVLLSGSGNVAQYAAEKAIELGAKVLTVSDSGGVLHFPEGMNREQLDALMTFKNDTRGRLSDFAEQHGLRFEA
GKTPWHVPADVALPCATQNELNGQDAERLLSNGVFCVAEGANMPSTLDAVDRFVAARILYAPGKASNAGGVATSGLEMSQ
NAMRMAWHHAEIDEKLHAIMKDIHGNCIHYGQKEDGYINYVEGANIAGFVKVADAMLAQGVI
>Mature_461_residues
AQAPHIASRKLRDIVTSRTLDSFLAGVSRRDPNQPEFFQAVKEVMMTLWPFVERNPRYGDQALLERLVEPERVIQFRVAW
TDDRGQVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKTDAEVMRFC
QALITELYRHLGPDTDVPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRG
REFDGLRVLLSGSGNVAQYAAEKAIELGAKVLTVSDSGGVLHFPEGMNREQLDALMTFKNDTRGRLSDFAEQHGLRFEAG
KTPWHVPADVALPCATQNELNGQDAERLLSNGVFCVAEGANMPSTLDAVDRFVAARILYAPGKASNAGGVATSGLEMSQN
AMRMAWHHAEIDEKLHAIMKDIHGNCIHYGQKEDGYINYVEGANIAGFVKVADAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=364, Percent_Identity=29.6703296703297, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI4885281, Length=381, Percent_Identity=28.8713910761155, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1788059, Length=445, Percent_Identity=68.9887640449438, Blast_Score=645, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=341, Percent_Identity=29.6187683284457, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=49.6688741721854, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319256, Length=455, Percent_Identity=49.4505494505495, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553475, Length=356, Percent_Identity=28.6516853932584, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45549226, Length=356, Percent_Identity=28.6516853932584, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24649283, Length=337, Percent_Identity=28.7833827893175, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24649460, Length=295, Percent_Identity=28.4745762711864, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 50490; Mature: 50359

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00436 PEROXIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAPHIASRKLRDIVTSRTLDSFLAGVSRRDPNQPEFFQAVKEVMMTLWPFVERNPRYG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DQALLERLVEPERVIQFRVAWTDDRGQVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVL
HHHHHHHHCCCCCEEEEEEEEECCCCCEEEEHEEEEECCCCCCCCCCCEEECCCCCHHHH
KFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKTDAEVMRFCQALITELYRHLGPDTDVPA
HHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRR
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHC
GREFDGLRVLLSGSGNVAQYAAEKAIELGAKVLTVSDSGGVLHFPEGMNREQLDALMTFK
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHC
NDTRGRLSDFAEQHGLRFEAGKTPWHVPADVALPCATQNELNGQDAERLLSNGVFCVAEG
CCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCEEEECC
ANMPSTLDAVDRFVAARILYAPGKASNAGGVATSGLEMSQNAMRMAWHHAEIDEKLHAIM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KDIHGNCIHYGQKEDGYINYVEGANIAGFVKVADAMLAQGVI
HHHCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQAPHIASRKLRDIVTSRTLDSFLAGVSRRDPNQPEFFQAVKEVMMTLWPFVERNPRYG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
DQALLERLVEPERVIQFRVAWTDDRGQVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVL
HHHHHHHHCCCCCEEEEEEEEECCCCCEEEEHEEEEECCCCCCCCCCCEEECCCCCHHHH
KFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKTDAEVMRFCQALITELYRHLGPDTDVPA
HHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRR
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHC
GREFDGLRVLLSGSGNVAQYAAEKAIELGAKVLTVSDSGGVLHFPEGMNREQLDALMTFK
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHC
NDTRGRLSDFAEQHGLRFEAGKTPWHVPADVALPCATQNELNGQDAERLLSNGVFCVAEG
CCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCEEEECC
ANMPSTLDAVDRFVAARILYAPGKASNAGGVATSGLEMSQNAMRMAWHHAEIDEKLHAIM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KDIHGNCIHYGQKEDGYINYVEGANIAGFVKVADAMLAQGVI
HHHCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]