Click here to switch to the map view.

The map label for this gene is mscS [H]

Identifier: 94313673

GI number: 94313673

Start: 1395411

End: 1396277

Strand: Direct

Name: mscS [H]

Synonym: Rmet_4751

Alternate gene names: 94313673

Gene position: 1395411-1396277 (Clockwise)

Preceding gene: 94313672

Following gene: 94313675

Centisome position: 54.08

GC content: 61.01

Gene sequence:

>867_bases
ATGGATACTTCTTCGTTGCAAAGCATCCGCGATGTGATTTATGGCTATGCCACTGTTTTCGGCGTCAAGATCCTTGCCGC
CCTCGCCTTCTGGGTGGTCGGCCGCTGGCTGATTCATTTCGTGGTGCGCATGGTCCAGAATTCGCTGGCCAGGCAGAAGG
TAGACCCGACGCTGCTGCGCTACGTTGGCTCGATCGTTACCGTCACGCTGAATGTCGTGCTGGTCATCGGCATCCTCGGC
TACTTCGGCATCCAGACTACGACGTTTGCGGCGCTGATCGCCGCAGCCGGTGTTGCTATCGGTATGGCCTGGTCTGGCCT
GATGTCGAACTTCGCGGCCGGCGCGTTCCTGGTGGTCCTGCGTCCGTTCAAGGTCGGGGATTTTGTATCCGTCGGTGGTG
TCACCGGCACGGTGCGCGAGATCGGCCTGTTCTCCACCACGCTCGACACGCCCGACAACGTCATGACGGTCGTCGGCAAC
AACAAGATCTTCAGCGACACGATCCAGAATTTCTCGGCCAATGCGTTCCGCCGGGTCGAATTGAAGGCGCAACTTGCCGG
GTCGACGGACGTGGCAGCCGCCACGGCTTTGCTCAAGGAAAAGATCGCGGCCATTCCGAACGTGCTGACCGAACCGCCCG
TCGATGTCGAGATCCTTGAATTCACGTTGGTTGGCCCGGTCTTGGCCGTCCGCCCCTACTGCCATACCGATCACTACTGG
CAGGTCTATTTCGATACCAATCGCACGATTCGCGACAGCTTCGGCCAGGCGGGCTTCCAGGCGCCGATGCCGGCCCAGGT
CGTGGTGATGCAGAACGCGCCGGCCGTATCGGTAGCATCGGGCACGCCGGCAAACCAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CACAGGCGTGTCTTGCAGCCCTGGCACCATCCACACCGATTTGCGCTGATTCTGACGTGCCTGATTTTCACTTTTCCATA
AAGAACTGGAGCGTGCCCCC

Downstream 100 bases:

>100_bases
GCTTGATCCGGCCAGGCAAGTTCGGCGGGATCAGGTTGCGGATTGGGAGCGCTGCCGCATACCTGCCCGCCCGTAGAGAC
GCAGCACGACCGCGCCACTG

Product: Mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MDTSSLQSIRDVIYGYATVFGVKILAALAFWVVGRWLIHFVVRMVQNSLARQKVDPTLLRYVGSIVTVTLNVVLVIGILG
YFGIQTTTFAALIAAAGVAIGMAWSGLMSNFAAGAFLVVLRPFKVGDFVSVGGVTGTVREIGLFSTTLDTPDNVMTVVGN
NKIFSDTIQNFSANAFRRVELKAQLAGSTDVAAATALLKEKIAAIPNVLTEPPVDVEILEFTLVGPVLAVRPYCHTDHYW
QVYFDTNRTIRDSFGQAGFQAPMPAQVVVMQNAPAVSVASGTPANQAA

Sequences:

>Translated_288_residues
MDTSSLQSIRDVIYGYATVFGVKILAALAFWVVGRWLIHFVVRMVQNSLARQKVDPTLLRYVGSIVTVTLNVVLVIGILG
YFGIQTTTFAALIAAAGVAIGMAWSGLMSNFAAGAFLVVLRPFKVGDFVSVGGVTGTVREIGLFSTTLDTPDNVMTVVGN
NKIFSDTIQNFSANAFRRVELKAQLAGSTDVAAATALLKEKIAAIPNVLTEPPVDVEILEFTLVGPVLAVRPYCHTDHYW
QVYFDTNRTIRDSFGQAGFQAPMPAQVVVMQNAPAVSVASGTPANQAA
>Mature_288_residues
MDTSSLQSIRDVIYGYATVFGVKILAALAFWVVGRWLIHFVVRMVQNSLARQKVDPTLLRYVGSIVTVTLNVVLVIGILG
YFGIQTTTFAALIAAAGVAIGMAWSGLMSNFAAGAFLVVLRPFKVGDFVSVGGVTGTVREIGLFSTTLDTPDNVMTVVGN
NKIFSDTIQNFSANAFRRVELKAQLAGSTDVAAATALLKEKIAAIPNVLTEPPVDVEILEFTLVGPVLAVRPYCHTDHYW
QVYFDTNRTIRDSFGQAGFQAPMPAQVVVMQNAPAVSVASGTPANQAA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=249, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787591, Length=239, Percent_Identity=25.9414225941423, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI2367355, Length=263, Percent_Identity=22.8136882129278, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30819; Mature: 30819

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSSLQSIRDVIYGYATVFGVKILAALAFWVVGRWLIHFVVRMVQNSLARQKVDPTLLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
YVGSIVTVTLNVVLVIGILGYFGIQTTTFAALIAAAGVAIGMAWSGLMSNFAAGAFLVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
RPFKVGDFVSVGGVTGTVREIGLFSTTLDTPDNVMTVVGNNKIFSDTIQNFSANAFRRVE
ECCCCCCEEEECCCCHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEE
LKAQLAGSTDVAAATALLKEKIAAIPNVLTEPPVDVEILEFTLVGPVLAVRPYCHTDHYW
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEE
QVYFDTNRTIRDSFGQAGFQAPMPAQVVVMQNAPAVSVASGTPANQAA
EEEECCCCHHHHHHCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MDTSSLQSIRDVIYGYATVFGVKILAALAFWVVGRWLIHFVVRMVQNSLARQKVDPTLLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
YVGSIVTVTLNVVLVIGILGYFGIQTTTFAALIAAAGVAIGMAWSGLMSNFAAGAFLVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
RPFKVGDFVSVGGVTGTVREIGLFSTTLDTPDNVMTVVGNNKIFSDTIQNFSANAFRRVE
ECCCCCCEEEECCCCHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEE
LKAQLAGSTDVAAATALLKEKIAAIPNVLTEPPVDVEILEFTLVGPVLAVRPYCHTDHYW
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCCEE
QVYFDTNRTIRDSFGQAGFQAPMPAQVVVMQNAPAVSVASGTPANQAA
EEEECCCCHHHHHHCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]