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The map label for this gene is amtB [C]

Identifier: 94313672

GI number: 94313672

Start: 1393967

End: 1395169

Strand: Direct

Name: amtB [C]

Synonym: Rmet_4750

Alternate gene names: 94313672

Gene position: 1393967-1395169 (Clockwise)

Preceding gene: 94313668

Following gene: 94313673

Centisome position: 54.03

GC content: 63.09

Gene sequence:

>1203_bases
ATGGACAACTTGAAAACCGGTACGGATGCAATGTTCATCCTGCTTGGCGCGATCATGGTGCTAGCCATGCACGCGGGATT
CGCATTCCTGGAGCTTGGCACCGTCCGCAAGAAGAATCAGGTCAATGCCCTGGTCAAGATCCTGGTCGATTTCGCGGTCT
CGACGATTTCATACTTTTTTATCGGTTATACGGTCGCCTACGGTGTGCAATTCATGTCCGGGGCAGATGTACTGGCGCAA
AAAAGCGGCTATGAGCTGGTGAAGTTCTTCTTCCTGGCCACATTTGCTGCGGCCATCCCGGCGATTATCTCGGGCGGTAT
TGCCGAGCGCTCGCGATTCAACCCGCAACTGGCAGCCACGTTCGTGCTGGTCGGTTTCGTCTACCCCTTCTTCGAAGGCA
TCGCGTGGAACAACCGATTCGGCATCCAGGACTGGATCGCCTCCGTGACCGGTGCGCCGTTTCATGATTTTGCCGGCTCG
GTGGTGGTCCACGCGGTCGGTGGCTGGATCGCGCTGCCGGCGGTGCTGCTGCTGGGCGCACGCCGCGGCCGCTATCAGAA
GGATGGCCGCATCAGCGCGCACCCGCCTTCGAATATCCCGTTCCTCGCGCTGGGCGCCTGGGTACTGGCGGTGGGCTGGT
TCGGCTTCAATGTCATGAGCGCGCAGACCGTCGACAAGATCAGCGGCCTTGTCGCGATCAACTCGCTGATGGCAATGGCG
GGCGGCACGCTGGCCGCGTGGTGGGCCGGCCGCAACGATCCGGGCTTTGCCTACAACGGCCCCCTGGCAGGCCTGGTCGC
CGTTTGCGCGGGCTCGGACCTAATGCATCCGCTGGGCGCGCTGATTACCGGCGCGGTGGCGGGTGTGCTGTTCGTTCATC
TGTTTACGCTGGCGCAGAACAAGTGGCACATCGACGACGTACTCGGTGTCTGGCCCCTGCACGGCCTGTGCGGCGCCTGG
GGCGGCATCGCCGCGGGCATCTTCGGCAGCCACGCGCTCGGCGGTATCGGCGGCGTCTCGATCGTGGCCCAGCTTATCGG
CACGGCCATGGGGATCGTGATTGCCCTGGTGGGCGGCACCGTGGTCTACGGCACGCTGAAGAAGGTCGTCGGCATTCGCC
TGGACGCTGAGGAGGAGTACAACGGCGCCGACCTGACACTGCACCGCATCTCCGCTACACCGGAGCGCGAAACGAGCTGG
TAA

Upstream 100 bases:

>100_bases
AGTGCGCCACGCACTGAGTTTGCGCGGTTTTTCGGCGTTCATGCCCAGATCTGGAACGGCTTTTGCATACCCATGCTCCC
ATTTTGAGCATGGGGCAACG

Downstream 100 bases:

>100_bases
TCGACGCGCTTGCAGCCTCGAATCGCCCCAAATAAAACGGCCGGAATCATCCGGCCGTTTTACTTTGTGTTCACAAAGAT
TAACTTGAACTTTGATCTCG

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDNLKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTISYFFIGYTVAYGVQFMSGADVLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLAATFVLVGFVYPFFEGIAWNNRFGIQDWIASVTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALITGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSIVAQLIGTAMGIVIALVGGTVVYGTLKKVVGIRLDAEEEYNGADLTLHRISATPERETSW

Sequences:

>Translated_400_residues
MDNLKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTISYFFIGYTVAYGVQFMSGADVLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLAATFVLVGFVYPFFEGIAWNNRFGIQDWIASVTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALITGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSIVAQLIGTAMGIVIALVGGTVVYGTLKKVVGIRLDAEEEYNGADLTLHRISATPERETSW
>Mature_400_residues
MDNLKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTISYFFIGYTVAYGVQFMSGADVLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLAATFVLVGFVYPFFEGIAWNNRFGIQDWIASVTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALITGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSIVAQLIGTAMGIVIALVGGTVVYGTLKKVVGIRLDAEEEYNGADLTLHRISATPERETSW

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=32.5925925925926, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=30.184331797235, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17550180, Length=439, Percent_Identity=28.7015945330296, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=430, Percent_Identity=27.6744186046512, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=438, Percent_Identity=26.027397260274, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321559, Length=360, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=406, Percent_Identity=25.3694581280788, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=29.3556085918854, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42146; Mature: 42146

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MDNLKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTISYFF
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGYTVAYGVQFMSGADVLAQKSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLAAT
HHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHH
FVLVGFVYPFFEGIAWNNRFGIQDWIASVTGAPFHDFAGSVVVHAVGGWIALPAVLLLGA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALITGAVAGVLFVHLFTLAQN
CCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KWHIDDVLGVWPLHGLCGAWGGIAAGIFGSHALGGIGGVSIVAQLIGTAMGIVIALVGGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVYGTLKKVVGIRLDAEEEYNGADLTLHRISATPERETSW
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]