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The map label for this gene is emrB [H]

Identifier: 94313574

GI number: 94313574

Start: 1282947

End: 1284473

Strand: Direct

Name: emrB [H]

Synonym: Rmet_4652

Alternate gene names: 94313574

Gene position: 1282947-1284473 (Clockwise)

Preceding gene: 94313573

Following gene: 94313575

Centisome position: 49.73

GC content: 62.67

Gene sequence:

>1527_bases
GTGTTCGCCTTTGCCTCGATGTGCGTGGGCATGTTTATCGCCCTGCTGGACATCCAGATCGTCTCCGCGTCGCTGCGTGA
TATTGGCGGTGGGCTGTCCGCTGGCGCGGACGAGACGGTGTGGGTCCAGACCGCCTACCTGATCGCCGAGATCATCGTCA
TCCCCCTATCAGGCTGGCTCGCGCGCGTCATGTCCACGCGCTGGCTGTTTGCCGCATCAGCCGCCGGATTCACGGCTACC
AGCCTGCTCTGCGGCATCGCGTGGAATATCCAGAGCATGATCGCCTTCCGCGCGGCGCAGGGCTTTCTGGGCGGATCGAT
GATCCCGATGGTCTTCACCACCGTCTTCGCGTTCTTCCAGGGACGTCAGCGTGTGGCCGCCGCCGCGATCATCGGTGGTC
TGGCGTCGCTGGCCCCCACCCTTGGCCCAACCATCGGCGGCTGGATCACCGACAATTTTTCGTGGCACTGGCTGTTTTTC
ATCAACCTGGTGCCGGGGATCTTCGTGACGATCGCGGTGCCGGCGCTGGTCAAGATCGACGAGCCCGACTGGTCGCTGCT
CAAGGGGGCGGACTACCTCGGCATCGTACTGATGGCGCTGTTTCTTGGCTGTCTCGAGTACACCCTGGAGGAAGGCCCCC
GCTGGGACTGGCTGGGCGACCGGACGATCCTGACCACGGCCTGGATCTCCGGGCTGGCCGGCATTGGTTTCCTGTGGCGT
TCGCTCACGTACAAGAACCCGGTGGTAGATCTACGCGCGCTGAAGGACATCAACTTCGCGCTCGGATGTTTCTTCTCGTT
TGTGGTCGGCATCGGAATTTTCGCGACGATATATCTGACGCCGCTGTTCCTGGGTCAGGTACGCGGATTCAGCGCCCTGC
AGATCGGGCTGGCGATCTTCTCGACGGGCCTGTTCCAGGTGCTATCGATACCGTTCTATGCCTACCTGGCGAACCGCGTG
GATCTACGCTGGCTGCTGATGTTCGGCCTCGCCCTGTTTGCGCTATCGATGTGGAATTTCGCGCCAATCACGCACGACTG
GGGCGGCGCGGAACTGCTGTTTCCGCAGGCGTTGCGGGGTATGGCGCAGCAGTTCGCCGTGGCGCCCACCGTTACGCTGA
CGCTTGGCGGCTTACCGCCGGCACGGCTCAAGCTGGCATCCGGGCTATTCAACCTGATGCGCAACCTGGGCGGGGCCATC
GGTATTGCCGCCTGCGCGACGATCCTCAACGACCGGACCAACCTGCACTTCCTGCGTCTGGCGGAACACCTGAACATTCG
CAACGAGTCCATGGCCGCTTTCCTCAACGAGGCAACCACGCACGCGGTGACGATGAACAACGAGATCAGTGATGGCAACG
CGGCCGCCCTGAAGCAACTCTGGGCGCTGACGATGCGCGAGGCCCACACGCTGACCTACGCCGATGCGTTCCTCGCGATC
TTTGTGTGTTTTGTCATCGCGGTTCCAATGGTCGTGCTAATGCGAAAGGTTGCGCCCCCGAGCGCGCCGTCTGCGGATGC
CCATTGA

Upstream 100 bases:

>100_bases
CGTCAGCACGCCTTCCGCCGCGCAGGCGTCATCGGTTCCACCCGTAGCGCCACCCGTCTCCCCGATACACTCGCCCGGCG
AACTGCCGATGGGAGCGCGG

Downstream 100 bases:

>100_bases
ACGCCGGCGGAATGTCGATTGACCGTCCATCGACCGTCCATCGACCGTCCATTGAGCTTCTATTGAGCTTCTATTGAGCG
GCTATCGAACGTCCATTGGT

Product: putative EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MFAFASMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTAYLIAEIIVIPLSGWLARVMSTRWLFAASAAGFTAT
SLLCGIAWNIQSMIAFRAAQGFLGGSMIPMVFTTVFAFFQGRQRVAAAAIIGGLASLAPTLGPTIGGWITDNFSWHWLFF
INLVPGIFVTIAVPALVKIDEPDWSLLKGADYLGIVLMALFLGCLEYTLEEGPRWDWLGDRTILTTAWISGLAGIGFLWR
SLTYKNPVVDLRALKDINFALGCFFSFVVGIGIFATIYLTPLFLGQVRGFSALQIGLAIFSTGLFQVLSIPFYAYLANRV
DLRWLLMFGLALFALSMWNFAPITHDWGGAELLFPQALRGMAQQFAVAPTVTLTLGGLPPARLKLASGLFNLMRNLGGAI
GIAACATILNDRTNLHFLRLAEHLNIRNESMAAFLNEATTHAVTMNNEISDGNAAALKQLWALTMREAHTLTYADAFLAI
FVCFVIAVPMVVLMRKVAPPSAPSADAH

Sequences:

>Translated_508_residues
MFAFASMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTAYLIAEIIVIPLSGWLARVMSTRWLFAASAAGFTAT
SLLCGIAWNIQSMIAFRAAQGFLGGSMIPMVFTTVFAFFQGRQRVAAAAIIGGLASLAPTLGPTIGGWITDNFSWHWLFF
INLVPGIFVTIAVPALVKIDEPDWSLLKGADYLGIVLMALFLGCLEYTLEEGPRWDWLGDRTILTTAWISGLAGIGFLWR
SLTYKNPVVDLRALKDINFALGCFFSFVVGIGIFATIYLTPLFLGQVRGFSALQIGLAIFSTGLFQVLSIPFYAYLANRV
DLRWLLMFGLALFALSMWNFAPITHDWGGAELLFPQALRGMAQQFAVAPTVTLTLGGLPPARLKLASGLFNLMRNLGGAI
GIAACATILNDRTNLHFLRLAEHLNIRNESMAAFLNEATTHAVTMNNEISDGNAAALKQLWALTMREAHTLTYADAFLAI
FVCFVIAVPMVVLMRKVAPPSAPSADAH
>Mature_508_residues
MFAFASMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTAYLIAEIIVIPLSGWLARVMSTRWLFAASAAGFTAT
SLLCGIAWNIQSMIAFRAAQGFLGGSMIPMVFTTVFAFFQGRQRVAAAAIIGGLASLAPTLGPTIGGWITDNFSWHWLFF
INLVPGIFVTIAVPALVKIDEPDWSLLKGADYLGIVLMALFLGCLEYTLEEGPRWDWLGDRTILTTAWISGLAGIGFLWR
SLTYKNPVVDLRALKDINFALGCFFSFVVGIGIFATIYLTPLFLGQVRGFSALQIGLAIFSTGLFQVLSIPFYAYLANRV
DLRWLLMFGLALFALSMWNFAPITHDWGGAELLFPQALRGMAQQFAVAPTVTLTLGGLPPARLKLASGLFNLMRNLGGAI
GIAACATILNDRTNLHFLRLAEHLNIRNESMAAFLNEATTHAVTMNNEISDGNAAALKQLWALTMREAHTLTYADAFLAI
FVCFVIAVPMVVLMRKVAPPSAPSADAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=436, Percent_Identity=30.5045871559633, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788710, Length=463, Percent_Identity=30.2375809935205, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=26.980198019802, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=22.4175824175824, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=24.3961352657005, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55041; Mature: 55041

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]