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The map label for this gene is emrA [H]

Identifier: 94313573

GI number: 94313573

Start: 1281697

End: 1282839

Strand: Direct

Name: emrA [H]

Synonym: Rmet_4651

Alternate gene names: 94313573

Gene position: 1281697-1282839 (Clockwise)

Preceding gene: 94313572

Following gene: 94313574

Centisome position: 49.68

GC content: 67.28

Gene sequence:

>1143_bases
ATGACAAGCACCACCACCGCGAGCCGCGCCCCCGCCGAGGCCGCTCCATCCACGGCAGCCCCGGCCAGGCGTGGCACCAA
CCGGCGGGCGTTGCTGATACGCGGCGCGGGCGCCTTGCTCGTCGTGACAGCCGCGGGATACGGCTATCACTGGTGGACTG
ATTCACGCTTCACCGAAGAGACCGACGACGCCTACGTAGGCGGTGACGTGACGCTGATTGCCGCGAAGGTGCCGGGCTAT
GTCGCGGAGGTTCTGGTGACCGACAATCAGCAGGTGCACGCGGGAGATCTGCTGGCACGCATCGACGACCGCGACTATCG
CGCAGCGCTGGCCAAGGCGGAGGGCGCAGTCGCGTCCGAGCAGGCGCTGATCGCCAATCTCGATGCACGGGAAAAACTGC
AGCAGGCGGTGATCGCCGAGGCCCGCGCCGGTGTGGCGGCGGTGGATGCGGAAACCAAGCGGGCGCGCGACGACCAGGCG
CGCTATGCCAGTCTGGTCGAGAAATCGGCGGTCTCGATCCAAAGCTCTCAGAAGGCCGATGCCGACTACAAGCAATCGGT
CGCCAACGGCCAGAAAGCGAAGGCCAGCGCGGAAGCCGCCCTGCGCCAACTCGATGTGATCGCCACGCAAAGGCAGCAGG
CGCAGGCATCGCTGACCCAGGCGATTGCCGAGCGCGACATCGCCCGACTCAATCTTGGCTACACCGAACTTCGCGCGCCA
GTCGACGGCACCGTGGGCAACCGCCGCGCGCGCGTCGGCGTCTACGCGCCTAACGGCGGCCAGTTGCTGTCGATCGTGCC
GGCACGCGGACTCTGGGTCGATGCCAATTTCAAGGAGGGCCAGCTTGCACATATGCGGCCCGGCATGCGTGCCACGATCG
TGGCCGACGTGATGCCCGGCAAGGTCTTCCACGGCCGCGTCGAAAGCCTGGCACCGGCCACGGGCGCGCAGTTCAGCGTG
CTGCCTCCGGAGAACGCCACCGGCAACTTCACGAAGATCGTGCAGCGCGTGCCGGTGCGGATCGTGCTGGATGACGAAGA
TGCCAGGCTCGGCACCCTGCGCCCGGGCCTGTCGGTGGTGGCGGAAGTCGATACGAAGGCCGCAAAGGTGGTGACGGCAG
ACACCGCAGCGAGCAAGTCATGA

Upstream 100 bases:

>100_bases
GCGCGCGCCTGGAACGCGCCGCTCAGCATCAGCAATCCGATTCGAATACCACCGCGCCCACGATGGGCGCGTGCTAACCA
TGGAACCACGGAGCACAGTC

Downstream 100 bases:

>100_bases
GCGCCGACGTCAGCACGCCTTCCGCCGCGCAGGCGTCATCGGTTCCACCCGTAGCGCCACCCGTCTCCCCGATACACTCG
CCCGGCGAACTGCCGATGGG

Product: multidrug resistance protein (HlyD family), EmrA-like secretion

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEETDDAYVGGDVTLIAAKVPGY
VAEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASEQALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQA
RYASLVEKSAVSIQSSQKADADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAP
VDGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPGKVFHGRVESLAPATGAQFSV
LPPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVVAEVDTKAAKVVTADTAASKS

Sequences:

>Translated_380_residues
MTSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEETDDAYVGGDVTLIAAKVPGY
VAEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASEQALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQA
RYASLVEKSAVSIQSSQKADADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAP
VDGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPGKVFHGRVESLAPATGAQFSV
LPPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVVAEVDTKAAKVVTADTAASKS
>Mature_379_residues
TSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEETDDAYVGGDVTLIAAKVPGYV
AEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASEQALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQAR
YASLVEKSAVSIQSSQKADADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAPV
DGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPGKVFHGRVESLAPATGAQFSVL
PPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVVAEVDTKAAKVVTADTAASKS

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=357, Percent_Identity=33.0532212885154, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789041, Length=331, Percent_Identity=34.441087613293, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790519, Length=333, Percent_Identity=30.9309309309309, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081951, Length=303, Percent_Identity=28.3828382838284, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790024, Length=249, Percent_Identity=28.1124497991968, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789637, Length=313, Percent_Identity=26.1980830670927, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790012, Length=249, Percent_Identity=28.5140562248996, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787013, Length=326, Percent_Identity=28.5276073619632, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40145; Mature: 40013

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEE
CCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCCCCC
TDDAYVGGDVTLIAAKVPGYVAEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASE
CCCEEECCCEEEEEECCCCEEEEEEEECCCEECCCHHHHHCCCCHHHHHHHHHCCCCCCC
QALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQARYASLVEKSAVSIQSSQKAD
CHHEECCCHHHHHHHHHHHHHHCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
ADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAP
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
VDGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPG
CCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCHHCCCCCEEEEEEECCCC
KVFHGRVESLAPATGAQFSVLPPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVV
CHHHCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCEEECCCCCCEE
AEVDTKAAKVVTADTAASKS
EECCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
TSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEE
CCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCCCCC
TDDAYVGGDVTLIAAKVPGYVAEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASE
CCCEEECCCEEEEEECCCCEEEEEEEECCCEECCCHHHHHCCCCHHHHHHHHHCCCCCCC
QALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQARYASLVEKSAVSIQSSQKAD
CHHEECCCHHHHHHHHHHHHHHCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
ADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAP
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
VDGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPG
CCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEECCCCCCCCHHCCCCCEEEEEEECCCC
KVFHGRVESLAPATGAQFSVLPPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVV
CHHHCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCEEECCCCCCEE
AEVDTKAAKVVTADTAASKS
EECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]