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The map label for this gene is oprJ [H]

Identifier: 94313437

GI number: 94313437

Start: 1115362

End: 1116924

Strand: Direct

Name: oprJ [H]

Synonym: Rmet_4512

Alternate gene names: 94313437

Gene position: 1115362-1116924 (Clockwise)

Preceding gene: 94313436

Following gene: 94313440

Centisome position: 43.23

GC content: 67.37

Gene sequence:

>1563_bases
ATGCCTAGAACAGGATTACCGATTTTCCCGCTGATGCTGCTCGGCGCCAGCCTTGCCGGTTGCGCGACGGCGTCGCTCGA
CCTTGCGCCGGAGCAGCCCGATCGTCCGTGGCAGCCTACGACCACGGCATCCGGAGAACTCGTGCCGGGCAAGCCCTCCG
CAACGGCCAACACCGGCAAGCCGGCCGACTACCGGCTGCCAGCCAATTCGGCGCTGGCGTCGATCACACCGCCAGCGGCA
CTCGATCCGGCGTATCCCTACACGTTGCCCGAGCTGATCGACATGGCCGAATCGGCGAATCCGATAACACGCGTGGCCTG
GAACGATGCACGCAACGCCGCGCTGGCTGCGGGGATCGCGAAGAGTAGCTATCTGCCGCAGATCTCCGCTTCCGCGCTGG
GCGGCTACCAGAATGGGCACGGTTCGGTATCGGGCGCACTCGGATCGGTCGGCACAAATTCGTCTGGCCACGGCGGCGTG
GGCGTGCTGTCGCTGCAATGGCTGCTGTTCGATTTCGGCGAGCGCGCGGGCATTGTCGAAGCCGCCGAACAGACTTCGCT
CGCGGCAAACATTGCGTTCACGGCCGCCCACCAGCGCATCATCCACGACGTCAGCGTGGCGTTCTACCGCTATCAGGCAG
CGCGCACCCGCGCGGGCACCGTGCAGCAGGCCATGGAAAACGCCGATGCCGTGCTGGCAGCCGCCAAGTCCCGCTTTCAG
CGCGGTATCGGTACCGTTGTGGAAGTTGCCCAGGCCACACAGAACCGCGCGCAGACCAACCTGGCGATGGTGCAGGCGCG
GGGCGCGGAGACCGATGCCTACCTGGCGCTGGTCACCGCGATCGGCATCTCGCCATTGTCGAAGCCGCGTATCGCCGAGA
TGCCAGTCCGTCCGCTGTCGCCGGCGCTGCATAGCTCCGTCGAGCAGATCGTGGAATCAGCCATCGCGCGGCGACCCGAC
GTGCTGGGCGCCTACGCAATCGAGCGGGCCAGCCAGGCCAAGGTCAAGGCGGCCGAAGCCGAATTCCTGCCCAAGGTGTT
CATGTCGGCGTTCACGTCGTATGCGTCGGGCGGGTCTTCGATTACGGCGATCCCCTCGGTGGCCCAGCAGGCGCCAACCG
TGAACCTGAATGGCTACCGCTATGGCGCCAGCGTGTTCCTCGGCATTACCGTGCCGATCTACGACGGCGGGCTGCGGTCG
GCGGTGCTGACGCAGGCGCGCAACGACGCCGACAGCGCGTCGACCAAACTCACGCGGGCCAAGGAAGAATCGGTGCGGCA
GGTTGTGGTCAGTCAGAGCGCGCTCGAAACCAGCCTTGCGGCATACGAAGCCGCCAAGGCCCTGGCCGACGCCGCCCAGA
TCACGTACGACGCCGCGTTCGCGGCCTACCGCAAGGGCGTCGGCACGGTCACGGATGCCAACCTGGCCCAGAACCAGCTA
CTGCTGGCGCGGAATGCATCGGCCGACGCTTACAGTGGCGCGCTATCGGCGGCAGCCGCGCTGGCCCTGGCCACGGGAGC
GGTCGGCGCGACACCTGAGCCGGGCGGCTGGGCACCGCGCTGA

Upstream 100 bases:

>100_bases
CAGCATTCCCCGCGCGACGCGCTGCTCGCCCTGGTTGACCGGCATCTCGCCGCTCCGCGCCAGGACGATCCGCCGGCCGC
CGCCACCGGATCACCGTTGC

Downstream 100 bases:

>100_bases
GCGCTGACTACGCGCCCGCCAAGGGCTGCTCCGGTCCGATCATGACCACGAGACTGCCTACGTGGATGACGGACTCCTCG
CCGATCCGGATGTCACCACC

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MPRTGLPIFPLMLLGASLAGCATASLDLAPEQPDRPWQPTTTASGELVPGKPSATANTGKPADYRLPANSALASITPPAA
LDPAYPYTLPELIDMAESANPITRVAWNDARNAALAAGIAKSSYLPQISASALGGYQNGHGSVSGALGSVGTNSSGHGGV
GVLSLQWLLFDFGERAGIVEAAEQTSLAANIAFTAAHQRIIHDVSVAFYRYQAARTRAGTVQQAMENADAVLAAAKSRFQ
RGIGTVVEVAQATQNRAQTNLAMVQARGAETDAYLALVTAIGISPLSKPRIAEMPVRPLSPALHSSVEQIVESAIARRPD
VLGAYAIERASQAKVKAAEAEFLPKVFMSAFTSYASGGSSITAIPSVAQQAPTVNLNGYRYGASVFLGITVPIYDGGLRS
AVLTQARNDADSASTKLTRAKEESVRQVVVSQSALETSLAAYEAAKALADAAQITYDAAFAAYRKGVGTVTDANLAQNQL
LLARNASADAYSGALSAAAALALATGAVGATPEPGGWAPR

Sequences:

>Translated_520_residues
MPRTGLPIFPLMLLGASLAGCATASLDLAPEQPDRPWQPTTTASGELVPGKPSATANTGKPADYRLPANSALASITPPAA
LDPAYPYTLPELIDMAESANPITRVAWNDARNAALAAGIAKSSYLPQISASALGGYQNGHGSVSGALGSVGTNSSGHGGV
GVLSLQWLLFDFGERAGIVEAAEQTSLAANIAFTAAHQRIIHDVSVAFYRYQAARTRAGTVQQAMENADAVLAAAKSRFQ
RGIGTVVEVAQATQNRAQTNLAMVQARGAETDAYLALVTAIGISPLSKPRIAEMPVRPLSPALHSSVEQIVESAIARRPD
VLGAYAIERASQAKVKAAEAEFLPKVFMSAFTSYASGGSSITAIPSVAQQAPTVNLNGYRYGASVFLGITVPIYDGGLRS
AVLTQARNDADSASTKLTRAKEESVRQVVVSQSALETSLAAYEAAKALADAAQITYDAAFAAYRKGVGTVTDANLAQNQL
LLARNASADAYSGALSAAAALALATGAVGATPEPGGWAPR
>Mature_519_residues
PRTGLPIFPLMLLGASLAGCATASLDLAPEQPDRPWQPTTTASGELVPGKPSATANTGKPADYRLPANSALASITPPAAL
DPAYPYTLPELIDMAESANPITRVAWNDARNAALAAGIAKSSYLPQISASALGGYQNGHGSVSGALGSVGTNSSGHGGVG
VLSLQWLLFDFGERAGIVEAAEQTSLAANIAFTAAHQRIIHDVSVAFYRYQAARTRAGTVQQAMENADAVLAAAKSRFQR
GIGTVVEVAQATQNRAQTNLAMVQARGAETDAYLALVTAIGISPLSKPRIAEMPVRPLSPALHSSVEQIVESAIARRPDV
LGAYAIERASQAKVKAAEAEFLPKVFMSAFTSYASGGSSITAIPSVAQQAPTVNLNGYRYGASVFLGITVPIYDGGLRSA
VLTQARNDADSASTKLTRAKEESVRQVVVSQSALETSLAAYEAAKALADAAQITYDAAFAAYRKGVGTVTDANLAQNQLL
LARNASADAYSGALSAAAALALATGAVGATPEPGGWAPR

Specific function: Channel-forming component of a multidrug resistance efflux pump [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53534; Mature: 53403

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTGLPIFPLMLLGASLAGCATASLDLAPEQPDRPWQPTTTASGELVPGKPSATANTGK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PADYRLPANSALASITPPAALDPAYPYTLPELIDMAESANPITRVAWNDARNAALAAGIA
CCCEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KSSYLPQISASALGGYQNGHGSVSGALGSVGTNSSGHGGVGVLSLQWLLFDFGERAGIVE
HHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
AAEQTSLAANIAFTAAHQRIIHDVSVAFYRYQAARTRAGTVQQAMENADAVLAAAKSRFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGIGTVVEVAQATQNRAQTNLAMVQARGAETDAYLALVTAIGISPLSKPRIAEMPVRPLS
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCC
PALHSSVEQIVESAIARRPDVLGAYAIERASQAKVKAAEAEFLPKVFMSAFTSYASGGSS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITAIPSVAQQAPTVNLNGYRYGASVFLGITVPIYDGGLRSAVLTQARNDADSASTKLTRA
EEHHHHHHHHCCEECCCCEEECCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHH
KEESVRQVVVSQSALETSLAAYEAAKALADAAQITYDAAFAAYRKGVGTVTDANLAQNQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCE
LLARNASADAYSGALSAAAALALATGAVGATPEPGGWAPR
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRTGLPIFPLMLLGASLAGCATASLDLAPEQPDRPWQPTTTASGELVPGKPSATANTGK
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PADYRLPANSALASITPPAALDPAYPYTLPELIDMAESANPITRVAWNDARNAALAAGIA
CCCEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
KSSYLPQISASALGGYQNGHGSVSGALGSVGTNSSGHGGVGVLSLQWLLFDFGERAGIVE
HHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
AAEQTSLAANIAFTAAHQRIIHDVSVAFYRYQAARTRAGTVQQAMENADAVLAAAKSRFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGIGTVVEVAQATQNRAQTNLAMVQARGAETDAYLALVTAIGISPLSKPRIAEMPVRPLS
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCC
PALHSSVEQIVESAIARRPDVLGAYAIERASQAKVKAAEAEFLPKVFMSAFTSYASGGSS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITAIPSVAQQAPTVNLNGYRYGASVFLGITVPIYDGGLRSAVLTQARNDADSASTKLTRA
EEHHHHHHHHCCEECCCCEEECCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHH
KEESVRQVVVSQSALETSLAAYEAAKALADAAQITYDAAFAAYRKGVGTVTDANLAQNQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCE
LLARNASADAYSGALSAAAALALATGAVGATPEPGGWAPR
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8878035; 10984043 [H]