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The map label for this gene is nreB [H]

Identifier: 94313440

GI number: 94313440

Start: 1120203

End: 1121354

Strand: Direct

Name: nreB [H]

Synonym: Rmet_4515

Alternate gene names: 94313440

Gene position: 1120203-1121354 (Clockwise)

Preceding gene: 94313437

Following gene: 94313441

Centisome position: 43.42

GC content: 65.19

Gene sequence:

>1152_bases
ATGGGGCCGGAAGCCGTGACGGGCACCGATGACAATGGCGACCGGCGAATCGCCGAAAACGAAACACGGCTGGCGGGAAT
CATCCGCTCGTCGATGGAAGCCATCATCTCGGTGGACGAAGAACAGCGCGTGGTGCTGTTCAACCCGATGGCGGAAAAGC
TGTTTGGCTTGCCGGCGGAACAGGCCATGGGCCGCCCCCTTGGCGATTTCATCCCTGCCCGTTTCCGCACTGCCCATGCG
GCGCACGTCACGCGTTTTGGCGTGACCGGTGTTTCCGACCGGCAGATGGGGCGCCAGCTCACGCTGCACGCCCTGCGTCA
GGACGGAACCGAATTCCCGATCGAAGCGTCGATCTCGCAGACCTCCGATGGTGCCGGACACAAGCTTTTTACGGTCATGC
TGCGCGACGTCACCGCTCGCGTGCGTGCCGAGACTGCGCTGCAGCGGTCCCGCGAGGAACTCCAGGCGCTGTCGGACAGC
ATCCTGGCAGCGCGCGAGGACGAAAAGCGACGCGTCGCGCGCGAGCTTCACGACGATCTGGGCCAGCGCCTGAGCGCGCT
GAAAATGGATCTGGTGATGCTGGAGACCGACTTGCGTACCGACTGCGGCGCTGTGCGTGCGCTCGGACAGGTCAGCGCCA
TGCACACTGTCATCGACGAAACCATCGCCGCCGTGCGCAGGATTTCATCCGATCTGCGGCCCGCGTTGCTCGATGAACTG
GGCCTCGCTGCCGCGCTGGACTGGCTGGCCAAGGATTTCAGCCGGCGCTACGCGATTCCGGTGAGCACGCGGACCCCCGA
GGAGATCGACGTCACCGAACAAGCCGCCACCGCTGTGTTCCGGATCGTCCAGGAGGCGCTCAACAACGTCGTGCATCATG
CGGACGCCAGCGAGGCCTGGGTAACGCTGACGCACCAGAACCCGAACCAGAACCACGATCACGACCAGTACGTGCTGCGC
GTGCACGATGACGGGCGCGGCTGGAACGGCCAGCCGAAGGACGGCGCGCGACGCTCGTTCGGCCTGCTTGGGATCCGGGA
ACGGGCACGCCTGCTGGGTGGCACGGTGTCCATCGAACATTCGCCCGGCGAGGGCTTCGAACTCGTCGTCACGTTTCCGG
GTCATCCGGATTGCAAGGAGACCCAGGCATGA

Upstream 100 bases:

>100_bases
GGCGCCACCCCCATGTTTCATCGCATAATTACGAATACAGATTCTGGCTGGAGCAGCAAGTCGCCCGCCACCGACCACGA
ATCCAGGACAGGAGTTGGGA

Downstream 100 bases:

>100_bases
TTCGCGTGATCATTGCAGACGATCACACTGTGGTGCGCAACGGATTGCGCCATATCCTCGAACGCACCAGCGACTTCGAG
GTGGTTGGCGAAGCAGCCCG

Product: Sensor kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MGPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAEQAMGRPLGDFIPARFRTAHA
AHVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQTSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDS
ILAAREDEKRRVARELHDDLGQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDEL
GLAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAWVTLTHQNPNQNHDHDQYVLR
VHDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEHSPGEGFELVVTFPGHPDCKETQA

Sequences:

>Translated_383_residues
MGPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAEQAMGRPLGDFIPARFRTAHA
AHVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQTSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDS
ILAAREDEKRRVARELHDDLGQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDEL
GLAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAWVTLTHQNPNQNHDHDQYVLR
VHDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEHSPGEGFELVVTFPGHPDCKETQA
>Mature_382_residues
GPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAEQAMGRPLGDFIPARFRTAHAA
HVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQTSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDSI
LAAREDEKRRVARELHDDLGQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDELG
LAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAWVTLTHQNPNQNHDHDQYVLRV
HDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEHSPGEGFELVVTFPGHPDCKETQA

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=231, Percent_Identity=33.7662337662338, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787474, Length=209, Percent_Identity=30.622009569378, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788812, Length=214, Percent_Identity=27.5700934579439, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42265; Mature: 42134

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCHH
QAMGRPLGDFIPARFRTAHAAHVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQ
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDSILAAREDEKRRVARELHDDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDEL
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GLAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAW
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VTLTHQNPNQNHDHDQYVLRVHDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEH
EEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCEEEEEC
SPGEGFELVVTFPGHPDCKETQA
CCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCHH
QAMGRPLGDFIPARFRTAHAAHVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQ
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDSILAAREDEKRRVARELHDDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDEL
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GLAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAW
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VTLTHQNPNQNHDHDQYVLRVHDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEH
EEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCHHHHHHHHHHHCCEEEEEC
SPGEGFELVVTFPGHPDCKETQA
CCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA