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The map label for this gene is citH [H]

Identifier: 94313431

GI number: 94313431

Start: 1108247

End: 1109551

Strand: Direct

Name: citH [H]

Synonym: Rmet_4506

Alternate gene names: 94313431

Gene position: 1108247-1109551 (Clockwise)

Preceding gene: 94313429

Following gene: 94313432

Centisome position: 42.95

GC content: 65.59

Gene sequence:

>1305_bases
ATGAATGACGTCACCCTGCAGCCGGCTCTTTCCCCCGCACCGGCTGCTGCCGAGCCACGACGAACCAGCCAGGCCCGGCT
GATCGCCGCCTGCTCCATCGGCAACGCGCTGGAAATGTATGACTTCACCGTCTACAGCTTCTTCGCGCTCATGATCGGCA
AGCTGTTCTTCCCCTCCGAGAGCGCCTACGGTTCGCTGCTGCTGGCCGTGGCAACGTTCGGTATCGGCTTTGTGATGCGG
CCGCTGGGCGGCTTCGTGATCGGCAACTATGCCGACCGCCACGGGCGCAAGGCTGCCATGACGCTGACCATCGGCCTGAT
GGTGGTTGGAACGCTTTGTCTGGCGGTGGCCCCGACGTACGCCACGGCGGGCCTGCTTGGCCCGCTGATCATTCTGGCGG
GGCGTCTGCTCCAGGGCTTCTCGCTGGGCGGCGAGATCGGTGCCTCCACCGCCATGCTGATGGAGGCTGGCGGTATCCGC
GGGCGTGGATTTCGTGTGAGCTGGCAGCTTGGCAGCCAGGGCATCGCCGCCCTGTGCGGCGCGCTGACAGCGGCGGTGCT
GTACGGCAGCCTGTCGACGGAAGCGCTGCAGAGCTGGGGATGGCGCGTGCCGTTCTTCCTGGGCTTGCTGATTGCGCCTG
TTGGCTTTTACATCCGATCGCATCTGGACGAAACGCACACGGCCGAGACCCATGCGCCAAGCCCGCTCGGCACGCTGTTC
CGCGAACACGGCACCCTGGTGGCCAAAGGCGTCCTCACCATCATCGGCGGTACCGTCGGCACGTATCTGGTGATCTATTT
CATGCCGACCTACATGATCCGGGAACTGCACCTCCCGGCCTCGCTATCGCTACTGGCAGGGTGCGTCACGGGTCTGACCA
GCTTTGCCGCGTCGCTCTGCTCCGGCTGGCTGGCCGACCGCCTCCAGCGCCGCAAACCGCTGGTGGTCGCCTCCATCCTG
TCCACCGTGGTGGCCCTCTATCCGGCCTTCTGGCTGATGACGCACTACCCCAGCGTGCCGCTGGTGCTGTCGCTCTCCGC
GCTGCTGACCGCGAGCCTGTATCTGGGAACGACGCCACTGCTGCTCATGATGATGGAGATGCTGCCGATCAAGGTACGGG
CCAGCGGCCTGTCGGTGATCTACGCCATCGGCGTCACGGTGTTCGGCGGCTCGTGCCAGTTCGTCGTCACCTGGCTGCTG
GCCCGCACCGGCAATCAGCTGTCCCCGGCGTTCTACATGATGGCCTGCAGCGCCGTGACACTACTGACCGTGCTGACCCT
GCGCGAAGTGCGCGAAACCCACTGA

Upstream 100 bases:

>100_bases
GGATGAATATTTGCGATTTTGTAGGTTCGCGACTTCCCCGAATACTTCATCGCCAAGGGTTCCTGCACAGAAGCCCGTCC
CCCACCCTCGGAGACCACCG

Downstream 100 bases:

>100_bases
CCTGCCAGCATCCGCCAGCACCCGCTAGCCCCTTTCCCCGCAAGGAACCCTGACATGACCAAAGCACCCACGCTCCAGCC
CTTTCAACTGCTGCCCCATC

Product: General substrate transporter:Major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNDVTLQPALSPAPAAAEPRRTSQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSESAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTYATAGLLGPLIILAGRLLQGFSLGGEIGASTAMLMEAGGIR
GRGFRVSWQLGSQGIAALCGALTAAVLYGSLSTEALQSWGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLF
REHGTLVAKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLCSGWLADRLQRRKPLVVASIL
STVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPLLLMMMEMLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNQLSPAFYMMACSAVTLLTVLTLREVRETH

Sequences:

>Translated_434_residues
MNDVTLQPALSPAPAAAEPRRTSQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSESAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTYATAGLLGPLIILAGRLLQGFSLGGEIGASTAMLMEAGGIR
GRGFRVSWQLGSQGIAALCGALTAAVLYGSLSTEALQSWGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLF
REHGTLVAKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLCSGWLADRLQRRKPLVVASIL
STVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPLLLMMMEMLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNQLSPAFYMMACSAVTLLTVLTLREVRETH
>Mature_434_residues
MNDVTLQPALSPAPAAAEPRRTSQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSESAYGSLLLAVATFGIGFVMR
PLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTYATAGLLGPLIILAGRLLQGFSLGGEIGASTAMLMEAGGIR
GRGFRVSWQLGSQGIAALCGALTAAVLYGSLSTEALQSWGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLF
REHGTLVAKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLCSGWLADRLQRRKPLVVASIL
STVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPLLLMMMEMLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLL
ARTGNQLSPAFYMMACSAVTLLTVLTLREVRETH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=31.353919239905, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788942, Length=391, Percent_Identity=33.7595907928389, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=27.7251184834123, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=30.0699300699301, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87082231, Length=248, Percent_Identity=28.6290322580645, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082404, Length=383, Percent_Identity=23.2375979112272, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46146; Mature: 46146

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVTLQPALSPAPAAAEPRRTSQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSE
CCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SAYGSLLLAVATFGIGFVMRPLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTY
CHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATAGLLGPLIILAGRLLQGFSLGGEIGASTAMLMEAGGIRGRGFRVSWQLGSQGIAALCG
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ALTAAVLYGSLSTEALQSWGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLF
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
REHGTLVAKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLC
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGWLADRLQRRKPLVVASILSTVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHH
LLMMMEMLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLLARTGNQLSPAFYMMACSAVT
HHHHHHHCCHHEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLTVLTLREVRETH
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNDVTLQPALSPAPAAAEPRRTSQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSE
CCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SAYGSLLLAVATFGIGFVMRPLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTY
CHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATAGLLGPLIILAGRLLQGFSLGGEIGASTAMLMEAGGIRGRGFRVSWQLGSQGIAALCG
HHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ALTAAVLYGSLSTEALQSWGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLF
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
REHGTLVAKGVLTIIGGTVGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLC
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SGWLADRLQRRKPLVVASILSTVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHH
LLMMMEMLPIKVRASGLSVIYAIGVTVFGGSCQFVVTWLLARTGNQLSPAFYMMACSAVT
HHHHHHHCCHHEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLTVLTLREVRETH
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]