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The map label for this gene is hipO [H]

Identifier: 94313432

GI number: 94313432

Start: 1109606

End: 1110817

Strand: Direct

Name: hipO [H]

Synonym: Rmet_4507

Alternate gene names: 94313432

Gene position: 1109606-1110817 (Clockwise)

Preceding gene: 94313431

Following gene: 94313434

Centisome position: 43.01

GC content: 65.76

Gene sequence:

>1212_bases
ATGACCAAAGCACCCACGCTCCAGCCCTTTCAACTGCTGCCCCATCTGCTGCCTCCGGTCGACATCGACGCCGAGACCTT
CGTCAACATCCGTCGTCAGATTCACGCCCAACCCGAACTCGGCTTCGAAGTCGGCGCCACCAGTACGCTGGTGACGCACC
TGCTGCAGAGCTGGGGCTATGACGTCCACACGGGTATCGGCAAGAGCGGGGTGGTAGGCCAGTTGAAACTGGGCAACGGC
GCGCGCCGGCTGGGCATTCGCGCCGACATGGACGCGCTGCCCATTGTCGAAGCCACCGGCCTGCCCTATGCCAGCCGCAA
CCACGGCAAGATGCACGCCTGCGGACACGACGGGCATACCGCGATCCTGCTGGCCGCGGCGAAAGCGCTGGCCGACAGCC
GCAGCTTCGACGGCACACTCAACCTGATTTTCCAGCCTGACGAAGAGAACCTCTGCGGCGCGCGCGCGATGATCGAAGAC
GGGCTCTTCGAGCGCTTCCCCTGCGACGCGGTTTTTGCGCTCCACAATATGCCGGGCGTGCCGGCGGGCAGTTTCCGTGT
GTTGCCGGGGCCGGTCAGCCTGTCTTCCGACGTGGCCGACATCACCATCAAGGGCGTGGGCGGGCATGGGGCGATGCCGC
ACCGCGCGCGTGATCCGATTGCGGCATCCGCCGCGATCGTGACGGCACTGCAAACCGTGGTAGCCCGCAATGTGGCGCCA
GACGACACCGCCGTCGTGTCGATCGGTTTCATTCGTGGCGGCGCCACCCACAACGTGATCCCCGAATCGGTAACTCTGGG
CGTCAACGTGCGCGCGGCGCGCGCGGAAACCCGGGCGATGGTGGAGCAGCGCATCCGCGAGATTGTCAGCCTGACCGCGC
AGGCCCACGGCGTCGAGGCACACATCGACTACCGCCAGCTCACCCCGCCCATGGTCAATACCGAAGCCGAAACCCGACTG
GCGCAGCGGATCTGCACCGATCTCGTGGGGACGGAGAACGTCGTCACGCAAGCCCCGAAGGGACTCAACGGCAGCGAGGA
TTTCGCCTGGATGCTCAATGAAGTCCCGGGCTGCTACCTGATCCTCGGCAACGGCGAAGGCGAATTCGGCGGCTGCATGG
TGCATAACCCCGGCTACGACTTCAACGATCAGGTGCTGCCGCTTGGCGCCGCCTGCTGGGTACGGCTGGCGCAGGCGTAC
CTGACAGCCTGA

Upstream 100 bases:

>100_bases
ACTACTGACCGTGCTGACCCTGCGCGAAGTGCGCGAAACCCACTGACCTGCCAGCATCCGCCAGCACCCGCTAGCCCCTT
TCCCCGCAAGGAACCCTGAC

Downstream 100 bases:

>100_bases
CGGCCTGACTGCCTGAGCCCCGACCGGCCGCCACCGGCCGCCCCGTCGGCGGCTAGTCCTTCGCCAGCTTTGCCGGACGC
ATGGTCGCGCTTTCCAGCAA

Product: Hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGYDVHTGIGKSGVVGQLKLGNG
ARRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVFALHNMPGVPAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
DDTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEAHIDYRQLTPPMVNTEAETRL
AQRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAY
LTA

Sequences:

>Translated_403_residues
MTKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGYDVHTGIGKSGVVGQLKLGNG
ARRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIED
GLFERFPCDAVFALHNMPGVPAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
DDTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEAHIDYRQLTPPMVNTEAETRL
AQRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAY
LTA
>Mature_402_residues
TKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGYDVHTGIGKSGVVGQLKLGNGA
RRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIEDG
LFERFPCDAVFALHNMPGVPAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAPD
DTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEAHIDYRQLTPPMVNTEAETRLA
QRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAYL
TA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=349, Percent_Identity=30.0859598853868, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42872; Mature: 42741

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGY
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCC
DVHTGIGKSGVVGQLKLGNGARRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHT
EEECCCCCCCCEEEEEECCCCCEECEECCCCCCCEEEECCCCCCCCCCCCEEECCCCCCH
AILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVFALHNMPGV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
CCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
HIDYRQLTPPMVNTEAETRLAQRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAYLTA
EEECCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGY
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCC
DVHTGIGKSGVVGQLKLGNGARRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHT
EEECCCCCCCCEEEEEECCCCCEECEECCCCCCCEEEECCCCCCCCCCCCEEECCCCCCH
AILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVFALHNMPGV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAP
CCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DDTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEA
CCCEEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
HIDYRQLTPPMVNTEAETRLAQRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYL
EECHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCEEE
ILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAYLTA
EEECCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]