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The map label for this gene is lutR [H]

Identifier: 94313372

GI number: 94313372

Start: 1044153

End: 1044911

Strand: Direct

Name: lutR [H]

Synonym: Rmet_4447

Alternate gene names: 94313372

Gene position: 1044153-1044911 (Clockwise)

Preceding gene: 94313371

Following gene: 94313373

Centisome position: 40.47

GC content: 63.24

Gene sequence:

>759_bases
ATGACGGAGATTCAGATGAAGGCCCCATTCGATACCGAAGCCCGCGGATTCACGAGCCTGCGCCGGCCAGACAACCTGCC
CGACGAGATCGCGCACCAGATTCGCCAGCGCATTCTCAACGGCTCGCTGGCGCATGGCCAACGGTTGCCCACGGAGCACG
AACTGGCCACGGCGTTCGACGTGAGCCGCAACGTCGTGCGGGAGGCCATCGCCCGGCTGAAACTGTCGGGCTACGTGGAA
ACGCGGCGCGGCACCGGCTCGTTCGTGGCCCACGGTATTGGGCAGCGCAATTTCGAGATCGTCACCGATGAACTGATGGA
GAAGGATGCGCTCGAGCATGTCTTCCAGTTGCGGGTCGAGATCGAATCGGGCGCGGCGGCGCTGGCGGCGATGTATCGCA
CTGATACGCAACTCGAGGCATTGCGCGTCGCGCTGGCGAAGGTCGACGAATCGGGCGGGGACCTGGAGAAGGGCACGGAT
AGCGCGCTCGATTTCCACCTTGCCGTGGGTAACGCCACCAACAACCCGTACTTCATCCGGTTGATGGCGCATCTGAGCCA
TGTATTGCACGACGCCGTGCGTACGCTGCGAACCGGATCGACGGGGACGGCGCGTATCGCGGAAGTCGAAAACGAACACC
ACGCCATCTTCGATGCCATCGCAGCTGGCGATGCGGAAGCGGCACGCACGGCCATGCGCCGGCATTTGACCAACGGAATC
GAACGACACCGGGCCAGACGGCCAAGAAAGGCAACATGA

Upstream 100 bases:

>100_bases
TCTGGACGCGGCGAAGGTCGAGGCGCTCGCCGCGCAGGGTGTCATCTGATTGGGCGCTAACATATCCGGCATGAATGTCC
CCGACACCGGGGCGCAAGAC

Downstream 100 bases:

>100_bases
ACAGCACACGCATTCTGGAAGGCGAAATTACCGATGCCTTGCTCGTGGATAAGCGGGGCAACACAGGCTGGATCACCTTC
AACGATCCCGGGCGCCACAA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTEIQMKAPFDTEARGFTSLRRPDNLPDEIAHQIRQRILNGSLAHGQRLPTEHELATAFDVSRNVVREAIARLKLSGYVE
TRRGTGSFVAHGIGQRNFEIVTDELMEKDALEHVFQLRVEIESGAAALAAMYRTDTQLEALRVALAKVDESGGDLEKGTD
SALDFHLAVGNATNNPYFIRLMAHLSHVLHDAVRTLRTGSTGTARIAEVENEHHAIFDAIAAGDAEAARTAMRRHLTNGI
ERHRARRPRKAT

Sequences:

>Translated_252_residues
MTEIQMKAPFDTEARGFTSLRRPDNLPDEIAHQIRQRILNGSLAHGQRLPTEHELATAFDVSRNVVREAIARLKLSGYVE
TRRGTGSFVAHGIGQRNFEIVTDELMEKDALEHVFQLRVEIESGAAALAAMYRTDTQLEALRVALAKVDESGGDLEKGTD
SALDFHLAVGNATNNPYFIRLMAHLSHVLHDAVRTLRTGSTGTARIAEVENEHHAIFDAIAAGDAEAARTAMRRHLTNGI
ERHRARRPRKAT
>Mature_251_residues
TEIQMKAPFDTEARGFTSLRRPDNLPDEIAHQIRQRILNGSLAHGQRLPTEHELATAFDVSRNVVREAIARLKLSGYVET
RRGTGSFVAHGIGQRNFEIVTDELMEKDALEHVFQLRVEIESGAAALAAMYRTDTQLEALRVALAKVDESGGDLEKGTDS
ALDFHLAVGNATNNPYFIRLMAHLSHVLHDAVRTLRTGSTGTARIAEVENEHHAIFDAIAAGDAEAARTAMRRHLTNGIE
RHRARRPRKAT

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=228, Percent_Identity=32.4561403508772, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790032, Length=221, Percent_Identity=31.6742081447964, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790780, Length=244, Percent_Identity=30.7377049180328, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87082208, Length=235, Percent_Identity=28.5106382978723, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI48994955, Length=214, Percent_Identity=30.8411214953271, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994961, Length=219, Percent_Identity=28.310502283105, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789621, Length=223, Percent_Identity=30.9417040358744, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27929; Mature: 27798

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIQMKAPFDTEARGFTSLRRPDNLPDEIAHQIRQRILNGSLAHGQRLPTEHELATAFD
CCCEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHH
VSRNVVREAIARLKLSGYVETRRGTGSFVAHGIGQRNFEIVTDELMEKDALEHVFQLRVE
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IESGAAALAAMYRTDTQLEALRVALAKVDESGGDLEKGTDSALDFHLAVGNATNNPYFIR
HCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHH
LMAHLSHVLHDAVRTLRTGSTGTARIAEVENEHHAIFDAIAAGDAEAARTAMRRHLTNGI
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERHRARRPRKAT
HHHHHCCCCCCC
>Mature Secondary Structure 
TEIQMKAPFDTEARGFTSLRRPDNLPDEIAHQIRQRILNGSLAHGQRLPTEHELATAFD
CCEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHH
VSRNVVREAIARLKLSGYVETRRGTGSFVAHGIGQRNFEIVTDELMEKDALEHVFQLRVE
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IESGAAALAAMYRTDTQLEALRVALAKVDESGGDLEKGTDSALDFHLAVGNATNNPYFIR
HCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHH
LMAHLSHVLHDAVRTLRTGSTGTARIAEVENEHHAIFDAIAAGDAEAARTAMRRHLTNGI
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERHRARRPRKAT
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]