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The map label for this gene is paaF [C]

Identifier: 94313373

GI number: 94313373

Start: 1044908

End: 1045732

Strand: Direct

Name: paaF [C]

Synonym: Rmet_4448

Alternate gene names: 94313373

Gene position: 1044908-1045732 (Clockwise)

Preceding gene: 94313372

Following gene: 94313374

Centisome position: 40.5

GC content: 64.0

Gene sequence:

>825_bases
ATGAACAGCACACGCATTCTGGAAGGCGAAATTACCGATGCCTTGCTCGTGGATAAGCGGGGCAACACAGGCTGGATCAC
CTTCAACGATCCCGGGCGCCACAACGCTGTCTCCTTCGATATGTGGGCGGCGGTGCCCAAGGCGCTGGCAGCATTCGAGC
AGGACGACGACATCCGCGCGGTCGTGCTCACCGGCGCTGGCGAACGCGCTTTCGTGAGCGGCGCCAACATTTCCCAGTTC
GACAAGCTGCGCTCGGGCGACGAAGCCGTGGCGGCCTACGAGCAGGTTGCAGAGGCCGCGCAGCTCGCGCTCTACGACTA
TGCCAAGCCCACGCTGGCACGCATCAAGGGTTATTGCATTGGCGGTGGGATGAACATCGCGCTGTGCTGCGATATCCGGA
TCGCATCGGCCGACAGCACCTTCGCGATTCCGGCTGGCAAGCTCGGGCTCGGGTACCGGCTGACCGCTATCCGCAACCTG
GTGACCACGGTCGGGGCGGCCAATGCGCTCGAGATCTTCCTGACCGCGAACCGCTACGCGGCTGCCGAAGCCAAGGCCCT
CGGACTGATCCATCACGTGACCGATACACCGGATGCGGCGGACCTCGATGCCGCGCTCCAGGTCCGCCTCGACCAGATCG
CCGCGAATGCGCCGCTGACGCTGCGCGCCGGCAAACGCATGATCCGCCAGTTGCAGCAGCTTGGTCCGGAAGTCGACATC
GCGGCCATGCAGCAACTGGTCATGCAGTGTTTCGCCAGCGACGACTATCGCGAAGGCAAGCGCGCCTTCGCTGAAAAACG
CAGCCCGGTTTTCACCGGGAAGTAG

Upstream 100 bases:

>100_bases
CCATCGCAGCTGGCGATGCGGAAGCGGCACGCACGGCCATGCGCCGGCATTTGACCAACGGAATCGAACGACACCGGGCC
AGACGGCCAAGAAAGGCAAC

Downstream 100 bases:

>100_bases
CGCGCGTCCCGGGAGACCACGGCTCCCGGCAGTATCGCCGCGCCGGACGTGGCGAACATATAAAACTACAGGAGACATGT
ATGAACAAGAGGCCGATTCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNSTRILEGEITDALLVDKRGNTGWITFNDPGRHNAVSFDMWAAVPKALAAFEQDDDIRAVVLTGAGERAFVSGANISQF
DKLRSGDEAVAAYEQVAEAAQLALYDYAKPTLARIKGYCIGGGMNIALCCDIRIASADSTFAIPAGKLGLGYRLTAIRNL
VTTVGAANALEIFLTANRYAAAEAKALGLIHHVTDTPDAADLDAALQVRLDQIAANAPLTLRAGKRMIRQLQQLGPEVDI
AAMQQLVMQCFASDDYREGKRAFAEKRSPVFTGK

Sequences:

>Translated_274_residues
MNSTRILEGEITDALLVDKRGNTGWITFNDPGRHNAVSFDMWAAVPKALAAFEQDDDIRAVVLTGAGERAFVSGANISQF
DKLRSGDEAVAAYEQVAEAAQLALYDYAKPTLARIKGYCIGGGMNIALCCDIRIASADSTFAIPAGKLGLGYRLTAIRNL
VTTVGAANALEIFLTANRYAAAEAKALGLIHHVTDTPDAADLDAALQVRLDQIAANAPLTLRAGKRMIRQLQQLGPEVDI
AAMQQLVMQCFASDDYREGKRAFAEKRSPVFTGK
>Mature_274_residues
MNSTRILEGEITDALLVDKRGNTGWITFNDPGRHNAVSFDMWAAVPKALAAFEQDDDIRAVVLTGAGERAFVSGANISQF
DKLRSGDEAVAAYEQVAEAAQLALYDYAKPTLARIKGYCIGGGMNIALCCDIRIASADSTFAIPAGKLGLGYRLTAIRNL
VTTVGAANALEIFLTANRYAAAEAKALGLIHHVTDTPDAADLDAALQVRLDQIAANAPLTLRAGKRMIRQLQQLGPEVDI
AAMQQLVMQCFASDDYREGKRAFAEKRSPVFTGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=28.6290322580645, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=201, Percent_Identity=26.3681592039801, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594471, Length=201, Percent_Identity=26.3681592039801, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=27.6315789473684, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=228, Percent_Identity=27.6315789473684, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=228, Percent_Identity=27.6315789473684, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI70995211, Length=214, Percent_Identity=25.7009345794392, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=27.3743016759777, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=23.7903225806452, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=28.4615384615385, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI221142681, Length=272, Percent_Identity=27.5735294117647, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=28.1746031746032, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=29.3436293436293, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788682, Length=219, Percent_Identity=28.7671232876712, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=31.496062992126, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=28.8235294117647, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=32.6693227091633, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=24.0458015267176, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=22.8682170542636, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=26.6094420600858, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=238, Percent_Identity=26.890756302521, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=24.3119266055046, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=31.0606060606061, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=30.5882352941176, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=30.5882352941176, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=23.0769230769231, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=27.319587628866, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=24, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571729, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29407; Mature: 29407

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTRILEGEITDALLVDKRGNTGWITFNDPGRHNAVSFDMWAAVPKALAAFEQDDDIRA
CCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEE
VVLTGAGERAFVSGANISQFDKLRSGDEAVAAYEQVAEAAQLALYDYAKPTLARIKGYCI
EEEECCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEE
GGGMNIALCCDIRIASADSTFAIPAGKLGLGYRLTAIRNLVTTVGAANALEIFLTANRYA
ECCCEEEEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
AAEAKALGLIHHVTDTPDAADLDAALQVRLDQIAANAPLTLRAGKRMIRQLQQLGPEVDI
HHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCH
AAMQQLVMQCFASDDYREGKRAFAEKRSPVFTGK
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNSTRILEGEITDALLVDKRGNTGWITFNDPGRHNAVSFDMWAAVPKALAAFEQDDDIRA
CCCCEEEECCCCCEEEEECCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEE
VVLTGAGERAFVSGANISQFDKLRSGDEAVAAYEQVAEAAQLALYDYAKPTLARIKGYCI
EEEECCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEE
GGGMNIALCCDIRIASADSTFAIPAGKLGLGYRLTAIRNLVTTVGAANALEIFLTANRYA
ECCCEEEEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
AAEAKALGLIHHVTDTPDAADLDAALQVRLDQIAANAPLTLRAGKRMIRQLQQLGPEVDI
HHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCH
AAMQQLVMQCFASDDYREGKRAFAEKRSPVFTGK
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA