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The map label for this gene is oxc [H]

Identifier: 94313285

GI number: 94313285

Start: 955561

End: 957483

Strand: Direct

Name: oxc [H]

Synonym: Rmet_4360

Alternate gene names: 94313285

Gene position: 955561-957483 (Clockwise)

Preceding gene: 94313282

Following gene: 94313286

Centisome position: 37.04

GC content: 63.39

Gene sequence:

>1923_bases
ATGGGATGGGCTGCTTGCTGCGGTGCGTCGTTGAAAGTCGGCAGCGGCCAGCATTTTGTTCTTGTTGTTTGGTGGTATAT
CACATACCGTATGTCATCAAGATCGGCTGCTTTCCGAAGCGCAACGATCTGCGATCCGACGATCCAGTCCAAACGAGGAG
ACAAAGACATGTCCGCTGTCGTATCCACCCGGCCCGAGACCACCACGGCTGAAGACACGCTGAAGCAAAAGTCCCGCGAC
GCGGGCGTCATATCCGGCGGCCATCTGGTGGCTCGCGCCCTCAAGAACGAGGGGGTGGATACGATCTTCACGCTGTGCGG
CGGGCACATCATCGACATCTACGACGGTTGCGTGGATGAGGGCATTCGCATCATCGACGTGCGCCACGAGCAGGTGGCCG
CGCACGCAGCAGATGGTTATGCGCGCCAGACCGGCAAGCTCGCCTGCGTTGTGACCACGGCAGGCCCTGGCTGCACGAAC
GCCGTCACGGGCGTGGCCACCGCGTTCCGTTCGGAAAGCCCGATCATCCATATCGGCGGCCAAGGCGCGCTGTCCCAGCA
CAAGATGGGATCGTTGCAGGATCTGCCGCACGTGGACATGATGGCGCCGATCACGAAGTTCGCGGCCACCATCCCCAGCA
CCGAGCGCGTGGCCGACATGATTGCGATGGCCGCCCGTGAGTGCTTCAACGGCGCGCCGGGGCCGGCCTACCTGGAAATC
CCGCGCGATGTGCTGGATCGTGAAGTCGATGTATCGCGTGCCGTGATTCCGCGCCCGGGCCACTATCGTGCCTCGACGAA
GTCGATCGGCGATCCGCGCGACATCGAGAAACTGGCCGACATCCTCGTCAACGCCGAACGTCCGGCAATTCTCTATGGCC
AGCAGGTCTGGACCGCGCGCGGGCATGAGGAAGCTGTCGACCTGCTGCGCGGTCTCGATATCCCCGGCTATTTCAACGGC
GCCGCACGTGGCCTGCTGCCCCCGGGCGATCCGCATCACTTCGACCGCACCCGTTCGCAGGCATTCGCCAATGCCGATGT
GCTGGTGATCGTCGGCACGCCGTTCGACTTCCGCATGGGCTACGGCAAGCGCATCAGCAAGGAAATCACGCTGGTGCAGA
TCGACATGGACTACCGCACGGTGGGCAAGAATCGCGAGATCGATCTGGGCCTGGTGGGCGATCCGGGCGCGATTCTTGGC
GCGGTGCTGCAGGCGGCCAGTGGCCGGATCAAGAACGATAAGCGCCTGGCGCGCAAGAAGTGGATGGGTCAGTTGACGGA
AGCCGAAGCTGTGGCCACGGAAAAGCTGATGCCGCTGTTCAAGTCGGAGAACAAGCCGATTCACCCGTATCGCGTGGCCT
ACGAACTCAACGAGTTCCTGGCCGACAACACGATCTATATCGGCGACGGTGGCGACGTGGTCACGATTTCCGCGCAGGCG
GTGCGTCCGCGCCGCCCGGGCCAGTGGATGGACCCGGGCGCGCTCGGATCGCTCGGTGTCGGTACCGGCTTTGCGATTGC
AGCGGGTGTGGCCAATCCAGGCAAGGAAGTGCTCTGCTACTACGGCGACGGATCGTTCGGCATGACCGCGTTCGACATGG
AAACGGCCAACCGCTTCGGCGTGCCGTACCTGGCCGTCATCGGCAATAACTCGGCGATGAACCAGATCCGCTACGGCCAG
CTTGCCAAGTACGGCGACGACCGTGGCAATGTCGGCAACCTGCTGTCCGACGTGCCGTTCTCCAAGTTCGCCGAGATGCT
GGGCGGATACGGCGAGGAGGTGCGCGAGCCGGGCCAGATCGCCGGTGCGCTGCAGCGCGGCCGCGAGTCGATCGCGAAGA
CCGGCAAGTCCGCCGTCATCAATATCTGGGTGGACCCGCGCGAGTACGCGCCGGGCACAAAGAACCAGACCATGTACAAG
TAA

Upstream 100 bases:

>100_bases
TGGAGAAGGCTGTTCCAGGTGGCAGACAGACTTCAGAAGACAATCGCATCAATGCCTTGTCATTGATCAGAAAGCGGCAT
TCTTTGCGGGTTAACGCCAA

Downstream 100 bases:

>100_bases
AGGCCGGTCGCCAGCACGACATTGCCTCAAGAGAATCCTCAAGGAGACAACGCCATGAACAACAAGGCACTGGAAGGGGT
CCGCATTCTGGACATGACGC

Product: thiamine pyrophosphate enzyme-like TPP binding region

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MGWAACCGASLKVGSGQHFVLVVWWYITYRMSSRSAAFRSATICDPTIQSKRGDKDMSAVVSTRPETTTAEDTLKQKSRD
AGVISGGHLVARALKNEGVDTIFTLCGGHIIDIYDGCVDEGIRIIDVRHEQVAAHAADGYARQTGKLACVVTTAGPGCTN
AVTGVATAFRSESPIIHIGGQGALSQHKMGSLQDLPHVDMMAPITKFAATIPSTERVADMIAMAARECFNGAPGPAYLEI
PRDVLDREVDVSRAVIPRPGHYRASTKSIGDPRDIEKLADILVNAERPAILYGQQVWTARGHEEAVDLLRGLDIPGYFNG
AARGLLPPGDPHHFDRTRSQAFANADVLVIVGTPFDFRMGYGKRISKEITLVQIDMDYRTVGKNREIDLGLVGDPGAILG
AVLQAASGRIKNDKRLARKKWMGQLTEAEAVATEKLMPLFKSENKPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQA
VRPRRPGQWMDPGALGSLGVGTGFAIAAGVANPGKEVLCYYGDGSFGMTAFDMETANRFGVPYLAVIGNNSAMNQIRYGQ
LAKYGDDRGNVGNLLSDVPFSKFAEMLGGYGEEVREPGQIAGALQRGRESIAKTGKSAVINIWVDPREYAPGTKNQTMYK

Sequences:

>Translated_640_residues
MGWAACCGASLKVGSGQHFVLVVWWYITYRMSSRSAAFRSATICDPTIQSKRGDKDMSAVVSTRPETTTAEDTLKQKSRD
AGVISGGHLVARALKNEGVDTIFTLCGGHIIDIYDGCVDEGIRIIDVRHEQVAAHAADGYARQTGKLACVVTTAGPGCTN
AVTGVATAFRSESPIIHIGGQGALSQHKMGSLQDLPHVDMMAPITKFAATIPSTERVADMIAMAARECFNGAPGPAYLEI
PRDVLDREVDVSRAVIPRPGHYRASTKSIGDPRDIEKLADILVNAERPAILYGQQVWTARGHEEAVDLLRGLDIPGYFNG
AARGLLPPGDPHHFDRTRSQAFANADVLVIVGTPFDFRMGYGKRISKEITLVQIDMDYRTVGKNREIDLGLVGDPGAILG
AVLQAASGRIKNDKRLARKKWMGQLTEAEAVATEKLMPLFKSENKPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQA
VRPRRPGQWMDPGALGSLGVGTGFAIAAGVANPGKEVLCYYGDGSFGMTAFDMETANRFGVPYLAVIGNNSAMNQIRYGQ
LAKYGDDRGNVGNLLSDVPFSKFAEMLGGYGEEVREPGQIAGALQRGRESIAKTGKSAVINIWVDPREYAPGTKNQTMYK
>Mature_639_residues
GWAACCGASLKVGSGQHFVLVVWWYITYRMSSRSAAFRSATICDPTIQSKRGDKDMSAVVSTRPETTTAEDTLKQKSRDA
GVISGGHLVARALKNEGVDTIFTLCGGHIIDIYDGCVDEGIRIIDVRHEQVAAHAADGYARQTGKLACVVTTAGPGCTNA
VTGVATAFRSESPIIHIGGQGALSQHKMGSLQDLPHVDMMAPITKFAATIPSTERVADMIAMAARECFNGAPGPAYLEIP
RDVLDREVDVSRAVIPRPGHYRASTKSIGDPRDIEKLADILVNAERPAILYGQQVWTARGHEEAVDLLRGLDIPGYFNGA
ARGLLPPGDPHHFDRTRSQAFANADVLVIVGTPFDFRMGYGKRISKEITLVQIDMDYRTVGKNREIDLGLVGDPGAILGA
VLQAASGRIKNDKRLARKKWMGQLTEAEAVATEKLMPLFKSENKPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQAV
RPRRPGQWMDPGALGSLGVGTGFAIAAGVANPGKEVLCYYGDGSFGMTAFDMETANRFGVPYLAVIGNNSAMNQIRYGQL
AKYGDDRGNVGNLLSDVPFSKFAEMLGGYGEEVREPGQIAGALQRGRESIAKTGKSAVINIWVDPREYAPGTKNQTMYK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=607, Percent_Identity=31.7957166392092, Blast_Score=275, Evalue=9e-74,
Organism=Homo sapiens, GI93004078, Length=575, Percent_Identity=26.6086956521739, Blast_Score=226, Evalue=5e-59,
Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=27.1095152603232, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=26.0714285714286, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1790104, Length=570, Percent_Identity=26.3157894736842, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=25.9856630824373, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787096, Length=533, Percent_Identity=25.140712945591, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=589, Percent_Identity=29.3718166383701, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=27.6223776223776, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17531301, Length=572, Percent_Identity=27.6223776223776, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320816, Length=554, Percent_Identity=24.3682310469314, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=26.3440860215054, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321524, Length=517, Percent_Identity=24.1779497098646, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=510, Percent_Identity=23.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=516, Percent_Identity=22.8682170542636, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=24.2857142857143, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 68982; Mature: 68851

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWAACCGASLKVGSGQHFVLVVWWYITYRMSSRSAAFRSATICDPTIQSKRGDKDMSAV
CCCHHHCCCCEEECCCCEEEEEEEEEHHHHHCCCCHHHCCCCCCCCCHHCCCCCHHHHHH
VSTRPETTTAEDTLKQKSRDAGVISGGHLVARALKNEGVDTIFTLCGGHIIDIYDGCVDE
HHCCCCCCCHHHHHHHHCCCCCEEECHHHHHHHHHHCCHHHHHHHHCCCEEEHHHHHHHC
GIRIIDVRHEQVAAHAADGYARQTGKLACVVTTAGPGCTNAVTGVATAFRSESPIIHIGG
CEEEEEECHHHHHHHHHCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECC
QGALSQHKMGSLQDLPHVDMMAPITKFAATIPSTERVADMIAMAARECFNGAPGPAYLEI
CCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
PRDVLDREVDVSRAVIPRPGHYRASTKSIGDPRDIEKLADILVNAERPAILYGQQVWTAR
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHEECC
GHEEAVDLLRGLDIPGYFNGAARGLLPPGDPHHFDRTRSQAFANADVLVIVGTPFDFRMG
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCC
YGKRISKEITLVQIDMDYRTVGKNREIDLGLVGDPGAILGAVLQAASGRIKNDKRLARKK
CCCCCCCEEEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHH
WMGQLTEAEAVATEKLMPLFKSENKPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHC
VRPRRPGQWMDPGALGSLGVGTGFAIAAGVANPGKEVLCYYGDGSFGMTAFDMETANRFG
CCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEEECCCCCCEEEEHHHHHHCC
VPYLAVIGNNSAMNQIRYGQLAKYGDDRGNVGNLLSDVPFSKFAEMLGGYGEEVREPGQI
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHCCCHHH
AGALQRGRESIAKTGKSAVINIWVDPREYAPGTKNQTMYK
HHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCCCCCCC
>Mature Secondary Structure 
GWAACCGASLKVGSGQHFVLVVWWYITYRMSSRSAAFRSATICDPTIQSKRGDKDMSAV
CCHHHCCCCEEECCCCEEEEEEEEEHHHHHCCCCHHHCCCCCCCCCHHCCCCCHHHHHH
VSTRPETTTAEDTLKQKSRDAGVISGGHLVARALKNEGVDTIFTLCGGHIIDIYDGCVDE
HHCCCCCCCHHHHHHHHCCCCCEEECHHHHHHHHHHCCHHHHHHHHCCCEEEHHHHHHHC
GIRIIDVRHEQVAAHAADGYARQTGKLACVVTTAGPGCTNAVTGVATAFRSESPIIHIGG
CEEEEEECHHHHHHHHHCCHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECC
QGALSQHKMGSLQDLPHVDMMAPITKFAATIPSTERVADMIAMAARECFNGAPGPAYLEI
CCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
PRDVLDREVDVSRAVIPRPGHYRASTKSIGDPRDIEKLADILVNAERPAILYGQQVWTAR
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHEECC
GHEEAVDLLRGLDIPGYFNGAARGLLPPGDPHHFDRTRSQAFANADVLVIVGTPFDFRMG
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCC
YGKRISKEITLVQIDMDYRTVGKNREIDLGLVGDPGAILGAVLQAASGRIKNDKRLARKK
CCCCCCCEEEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHH
WMGQLTEAEAVATEKLMPLFKSENKPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHC
VRPRRPGQWMDPGALGSLGVGTGFAIAAGVANPGKEVLCYYGDGSFGMTAFDMETANRFG
CCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEEECCCCCCEEEEHHHHHHCC
VPYLAVIGNNSAMNQIRYGQLAKYGDDRGNVGNLLSDVPFSKFAEMLGGYGEEVREPGQI
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHCCCHHH
AGALQRGRESIAKTGKSAVINIWVDPREYAPGTKNQTMYK
HHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]