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The map label for this gene is nadB [C]

Identifier: 94313227

GI number: 94313227

Start: 895097

End: 896746

Strand: Reverse

Name: nadB [C]

Synonym: Rmet_4301

Alternate gene names: 94313227

Gene position: 896746-895097 (Counterclockwise)

Preceding gene: 94313228

Following gene: 94313226

Centisome position: 34.76

GC content: 66.12

Gene sequence:

>1650_bases
ATGCCTGCCGGCATCCCTTACGACGAGGCGCGCAAGCGCTACCGGCCCGGCACGGCGCCGGCGGTGCGCAGTGACAACAC
CTCGGCCGAAGCGCTCCTCAAGGGCTACCACCCCGACCACGGGCCGGACGCCCGCGTTGCCCTCGCCGTCGGCGTGAACC
GCGGCGATTCCTGCCAGCCGGATCTCGCGCGGCATCTGCAAGCCAATGCGCTGATCGACGACGTCGACATTGCCGGTGCC
CAACTCATGGCCACCGATGTGTTGGTCATTGGTGGTGGAGGTGGCGGGTGCGCCGCGGCACTCACCGCGGCAAAGCATGG
GGCGCGGGTCATCCTCGCCACCAAGCTTCGCCTCGGCGACAGCAACACTGTGATGGCCGAAGGCGGGATACAGGCCGCCA
TCGGAGAGGACGACAGCCCTCAATTGCACTTCGAGGACACGCTGCGTGCCGGACACTTCTGCGGCGATCCCGCACTCGTA
GCGCAAATGGTGATGGATGGTCCGGATGTCATTCGCTGGCTGATCCAGCTCGGCATGATGTTTGACCAGGAAGAGGATCG
GCCAATCGGCGGCAACCTTCTGCGCAAAAAGCCCGGCGGCGCCTCGGCCGCGCGCATTCTTTCCTACCGGGACTACACCG
GGCTGGAGATGATGCGGGTGCTGCGGGAAGCGGTGGACCTGGATCCCGGCATCGAGGTATGGAACCGCTGCCCGGCGGTC
GAGCTGCTGTCCGACGAGCGGGGAAACTGCGCAGGGGCCGTGATCTACAACCTCGAATCACGCACCTTTGTGCTAGTGCG
CGCTGCTGCCGTGATCATTGCTACCGGCGGAGCCGGGCGCCTGCACCTGAATTCGTTTCCAACATCCAATCATTTCGGTG
CCACCGCCGACGGCCTCGTGCTCGCCTACCGGATCGGAGCGCGCCTGCGCGAGCTCGACTCGTTCCAGTACCACCCCACC
GGCATTGCGTACCCTCCCCACCTTGCAGGCGGACTCATCTCCGAGGCCGCGCGCTCCGCGGGCGCCAAGCTGATCAACGG
CGAGGGTGAGCGCTTTGTGGACGAACTCAAGGCACGGGACATCGTGGCCTCGGCGATATTGCGCGAATGTGCGCAGGGGC
GCGGCATCATCCGTGAGGGGCAGGTGGGCGTGCTGCTCGACACGCCGACGCTCGAAATGGAGCATCCCGGCATCCTGGAC
AAGCGCCTTGTGACGTTGAGCCACCTTGCGCACAAGTGCGGTCACGATGCAGCGAAGGAACCCTTCCTTGTCTACCCAAC
CCTTCACTACCAGAACGGCGGCGTTGCGATCGACGGACATGGGACCACCAGCGTATCCGGACTGTACTGCGTGGGTGAGG
TAGCGGGAGGCATCCACGGGCGCAACCGGCTGATGGGGAATGCGCTGCTCGACATCCTAAGCATGGGTCGGCGCGCGGGC
GCGTCGGCAGCGGAGGCGAAGCGTTCGGCTGTGGCCAGCCGCGCAGGGATCGGCCACGTGCACGCATGGCAGCGGGAACT
GACGCTGGCTGGGCTACCTTTGCACGTCAAGGCACCGATTCTCTTTCCTGGCTACGCGCATTTCGATCTGCGCGTCAATG
CAGGCCTGCGCGCTGGCGTCCGCGGCCGGGCCGCTGGCGGTGTCCGGTGA

Upstream 100 bases:

>100_bases
CGAATCTGATGCGACGGCTGCGGGAGATCGAGCGTGGCGACATGACAATCGATCTCGACGCGCCGGGCGCCCAGCCACCA
CACTGACCGAGACACCCCTC

Downstream 100 bases:

>100_bases
GCCGTAGACGGAGCGCGCGATGGAAAAGCTGAATCAGGTTCTCCTGAGGGACGATGTCCGCGTTGGTGCGGACCTGGAGG
CATGGCTCGCAATTGGCGGA

Product: fumarate reductase/succinate dehydrogenase flavoprotein-like protein

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPAGIPYDEARKRYRPGTAPAVRSDNTSAEALLKGYHPDHGPDARVALAVGVNRGDSCQPDLARHLQANALIDDVDIAGA
QLMATDVLVIGGGGGGCAAALTAAKHGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGDPALV
AQMVMDGPDVIRWLIQLGMMFDQEEDRPIGGNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDPGIEVWNRCPAV
ELLSDERGNCAGAVIYNLESRTFVLVRAAAVIIATGGAGRLHLNSFPTSNHFGATADGLVLAYRIGARLRELDSFQYHPT
GIAYPPHLAGGLISEAARSAGAKLINGEGERFVDELKARDIVASAILRECAQGRGIIREGQVGVLLDTPTLEMEHPGILD
KRLVTLSHLAHKCGHDAAKEPFLVYPTLHYQNGGVAIDGHGTTSVSGLYCVGEVAGGIHGRNRLMGNALLDILSMGRRAG
ASAAEAKRSAVASRAGIGHVHAWQRELTLAGLPLHVKAPILFPGYAHFDLRVNAGLRAGVRGRAAGGVR

Sequences:

>Translated_549_residues
MPAGIPYDEARKRYRPGTAPAVRSDNTSAEALLKGYHPDHGPDARVALAVGVNRGDSCQPDLARHLQANALIDDVDIAGA
QLMATDVLVIGGGGGGCAAALTAAKHGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGDPALV
AQMVMDGPDVIRWLIQLGMMFDQEEDRPIGGNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDPGIEVWNRCPAV
ELLSDERGNCAGAVIYNLESRTFVLVRAAAVIIATGGAGRLHLNSFPTSNHFGATADGLVLAYRIGARLRELDSFQYHPT
GIAYPPHLAGGLISEAARSAGAKLINGEGERFVDELKARDIVASAILRECAQGRGIIREGQVGVLLDTPTLEMEHPGILD
KRLVTLSHLAHKCGHDAAKEPFLVYPTLHYQNGGVAIDGHGTTSVSGLYCVGEVAGGIHGRNRLMGNALLDILSMGRRAG
ASAAEAKRSAVASRAGIGHVHAWQRELTLAGLPLHVKAPILFPGYAHFDLRVNAGLRAGVRGRAAGGVR
>Mature_548_residues
PAGIPYDEARKRYRPGTAPAVRSDNTSAEALLKGYHPDHGPDARVALAVGVNRGDSCQPDLARHLQANALIDDVDIAGAQ
LMATDVLVIGGGGGGCAAALTAAKHGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGDPALVA
QMVMDGPDVIRWLIQLGMMFDQEEDRPIGGNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDPGIEVWNRCPAVE
LLSDERGNCAGAVIYNLESRTFVLVRAAAVIIATGGAGRLHLNSFPTSNHFGATADGLVLAYRIGARLRELDSFQYHPTG
IAYPPHLAGGLISEAARSAGAKLINGEGERFVDELKARDIVASAILRECAQGRGIIREGQVGVLLDTPTLEMEHPGILDK
RLVTLSHLAHKCGHDAAKEPFLVYPTLHYQNGGVAIDGHGTTSVSGLYCVGEVAGGIHGRNRLMGNALLDILSMGRRAGA
SAAEAKRSAVASRAGIGHVHAWQRELTLAGLPLHVKAPILFPGYAHFDLRVNAGLRAGVRGRAAGGVR

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=414, Percent_Identity=34.5410628019324, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788928, Length=415, Percent_Identity=34.9397590361446, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786942, Length=429, Percent_Identity=34.4988344988345, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790597, Length=406, Percent_Identity=32.0197044334975, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17505833, Length=405, Percent_Identity=33.0864197530864, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17550100, Length=405, Percent_Identity=32.5925925925926, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986328, Length=473, Percent_Identity=28.1183932346723, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322701, Length=431, Percent_Identity=34.1067285382831, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322416, Length=446, Percent_Identity=33.4080717488789, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=28.781512605042, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322511, Length=485, Percent_Identity=24.9484536082474, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17137288, Length=436, Percent_Identity=35.0917431192661, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655642, Length=436, Percent_Identity=35.0917431192661, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24655647, Length=436, Percent_Identity=35.0917431192661, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24663005, Length=469, Percent_Identity=31.1300639658849, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 57998; Mature: 57867

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGIPYDEARKRYRPGTAPAVRSDNTSAEALLKGYHPDHGPDARVALAVGVNRGDSCQP
CCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCH
DLARHLQANALIDDVDIAGAQLMATDVLVIGGGGGGCAAALTAAKHGARVILATKLRLGD
HHHHHHHHCCEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGDPALVAQMVMDGPDVIRWLIQLGMM
CCEEEECCCEEEECCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
FDQEEDRPIGGNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDPGIEVWNRCPAV
HCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCE
ELLSDERGNCAGAVIYNLESRTFVLVRAAAVIIATGGAGRLHLNSFPTSNHFGATADGLV
EECCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEE
LAYRIGARLRELDSFQYHPTGIAYPPHLAGGLISEAARSAGAKLINGEGERFVDELKARD
EEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
IVASAILRECAQGRGIIREGQVGVLLDTPTLEMEHPGILDKRLVTLSHLAHKCGHDAAKE
HHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PFLVYPTLHYQNGGVAIDGHGTTSVSGLYCVGEVAGGIHGRNRLMGNALLDILSMGRRAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ASAAEAKRSAVASRAGIGHVHAWQRELTLAGLPLHVKAPILFPGYAHFDLRVNAGLRAGV
CCHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCEEEECCEECCCCEEEEEEECCCCCCCC
RGRAAGGVR
CCCCCCCCC
>Mature Secondary Structure 
PAGIPYDEARKRYRPGTAPAVRSDNTSAEALLKGYHPDHGPDARVALAVGVNRGDSCQP
CCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCH
DLARHLQANALIDDVDIAGAQLMATDVLVIGGGGGGCAAALTAAKHGARVILATKLRLGD
HHHHHHHHCCEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGDPALVAQMVMDGPDVIRWLIQLGMM
CCEEEECCCEEEECCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
FDQEEDRPIGGNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDPGIEVWNRCPAV
HCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCE
ELLSDERGNCAGAVIYNLESRTFVLVRAAAVIIATGGAGRLHLNSFPTSNHFGATADGLV
EECCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEE
LAYRIGARLRELDSFQYHPTGIAYPPHLAGGLISEAARSAGAKLINGEGERFVDELKARD
EEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
IVASAILRECAQGRGIIREGQVGVLLDTPTLEMEHPGILDKRLVTLSHLAHKCGHDAAKE
HHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PFLVYPTLHYQNGGVAIDGHGTTSVSGLYCVGEVAGGIHGRNRLMGNALLDILSMGRRAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ASAAEAKRSAVASRAGIGHVHAWQRELTLAGLPLHVKAPILFPGYAHFDLRVNAGLRAGV
CCHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCEEEECCEECCCCEEEEEEECCCCCCCC
RGRAAGGVR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]