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The map label for this gene is nuoF [H]

Identifier: 94313226

GI number: 94313226

Start: 893833

End: 895077

Strand: Reverse

Name: nuoF [H]

Synonym: Rmet_4300

Alternate gene names: 94313226

Gene position: 895077-893833 (Counterclockwise)

Preceding gene: 94313227

Following gene: 94313225

Centisome position: 34.69

GC content: 61.85

Gene sequence:

>1245_bases
ATGGAAAAGCTGAATCAGGTTCTCCTGAGGGACGATGTCCGCGTTGGTGCGGACCTGGAGGCATGGCTCGCAATTGGCGG
AGGAGAGGGGCTGGCCAAGGCCTTGTGCGATCCAGGCGCAGTCATAGGCGAGATCGAGCAGGCCGATCTGCGCGGAATGG
GCGGCGCCGGATTTGCGACCCATCGCAAATGGGCGCCGGTTGCCGCCGCGGCTGACGGCGACAAAACCATCATCTGCAAC
GGCAACGAAGATGAGCCGGGGACCTTCAAGGACCGCTTCCTGCTGGAGCACACGCCACATCAGGTGATCGAGGGTGCCCT
CATCGCCGCGGCCGCCACGCGTGCCAACCATATTGTCCTTTACGTCAACCCTCACCAGCAGCAGGCCATTGCGGTCATAC
GACAGGCCGTCGGGCAATGGCAGGCGCACCCCCGGTACGCCGAACTCGAGCGACTGCTGGGCGCCCCCCTGTCGCTTGGC
GTTGTACCGAGTTCAGGGCTATACATCGGGGGCGAGGAGACGGCGGTGATCGCAAGCGTCGAGGGCGGATTCCCGTTCCC
GCGGCGCAAGCCGCCCTTTCCCAGTCAACAAGGCGTGCATGGCGCGCCAAGCATCGTCAACAACGTCGAAACGCTAGCGC
ATATACCAGGAATTCTTCGCCACGGTGCTCAGTGGTATCGCGATCTCGGCATCGGTAACGCAACCGGAACCAAACTCTAT
TCACTCTCTGGCGACGTATTGCGCCCCGGTCTGTATGAACTACCAATGGGAACGAGCCTGGAGTCCCTGGTGTTCGAGCA
CGGTGGCGGCATGTTGCAAGGCAAGGAGTTCAAGGCCGTCTTTACAGGGGGGCCCTCGAATACTCTGCTGACGAAGCGTG
ACCTCGATGTCGCCCTGGACTTTGATTCGGTGCGACTAAGACGCTCACGTCTGGGAACGGGGGCGATGATCGTTGTATCG
GAAGGCACCAGCATTGTCCGCAAGGTCGCTGAATTTGTGAGCTTCTTCGCGCAAGGATCGTGCGGCCAATGCCCACCGTG
CAAAGGTGGCAGCTTCCAGTTGATGCGATTGCTGAACCGCATCGATACGGGCCGCGGTGTGCATGCCGATCTGGCAGCGC
TGGAGAATCTGTGCCGCATCCTACCCGGCAGCGGCCGCTGCGGCCTCATCGACGGCGCCGTGACGGTGGTGGAGAGTTCC
CTGCACCAGTTCCGTGAGGAGTACGAGGCGCTGCTTATGGCATAG

Upstream 100 bases:

>100_bases
GGCTACGCGCATTTCGATCTGCGCGTCAATGCAGGCCTGCGCGCTGGCGTCCGCGGCCGGGCCGCTGGCGGTGTCCGGTG
AGCCGTAGACGGAGCGCGCG

Downstream 100 bases:

>100_bases
GCGGCGAGGATAGAGGGCAGCGATGAAATGCACGGCACCTTAGTCCCTCACTCCTGCTATGTCAGCGGGGCTGATCCGAC
CCGACTGGCGGTCTGAACCA

Product: NADH:ubiquinone oxidoreductase complex I, chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFATHRKWAPVAAAADGDKTIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVLYVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLG
VVPSSGLYIGGEETAVIASVEGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLY
SLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRLRRSRLGTGAMIVVS
EGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNRIDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESS
LHQFREEYEALLMA

Sequences:

>Translated_414_residues
MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFATHRKWAPVAAAADGDKTIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVLYVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLG
VVPSSGLYIGGEETAVIASVEGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLY
SLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRLRRSRLGTGAMIVVS
EGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNRIDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESS
LHQFREEYEALLMA
>Mature_414_residues
MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFATHRKWAPVAAAADGDKTIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVLYVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLG
VVPSSGLYIGGEETAVIASVEGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLY
SLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRLRRSRLGTGAMIVVS
EGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNRIDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESS
LHQFREEYEALLMA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=375, Percent_Identity=36.5333333333333, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI20149568, Length=375, Percent_Identity=36.5333333333333, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1788620, Length=406, Percent_Identity=38.423645320197, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17531727, Length=372, Percent_Identity=35.752688172043, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550452, Length=368, Percent_Identity=36.1413043478261, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=35.2, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24653819, Length=382, Percent_Identity=32.7225130890052, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24653817, Length=343, Percent_Identity=33.2361516034985, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 44125; Mature: 44125

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFAT
CCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCC
HRKWAPVAAAADGDKTIICNGNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVL
CCCCCCEEEECCCCCEEEECCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHCCCEEEE
YVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLGVVPSSGLYIGGEETAVIASV
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCEEECCCCEEEEEEE
EGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
SLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALD
ECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCEEEEC
FDSVRLRRSRLGTGAMIVVSEGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESSLHQFREEYEALLMA
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFAT
CCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCC
HRKWAPVAAAADGDKTIICNGNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVL
CCCCCCEEEECCCCCEEEECCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHHCCCEEEE
YVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLGVVPSSGLYIGGEETAVIASV
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCEEECCCCEEEEEEE
EGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
SLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALD
ECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCEEEEC
FDSVRLRRSRLGTGAMIVVSEGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESSLHQFREEYEALLMA
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]