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The map label for this gene is dctA

Identifier: 94313225

GI number: 94313225

Start: 892359

End: 893717

Strand: Reverse

Name: dctA

Synonym: Rmet_4299

Alternate gene names: 94313225

Gene position: 893717-892359 (Counterclockwise)

Preceding gene: 94313226

Following gene: 94313223

Centisome position: 34.64

GC content: 56.95

Gene sequence:

>1359_bases
ATGGAAAAAGTCGACAACCGAAAGCCGCTTTACAGATCCCTCTACTTCCAGGTCATCGTTGCCATCATCAGCGGCGTGGT
CCTCGGCCACTATTATCCAACTGCCGGAGAGGCCATGAAGCCGTTGGGTGACGGGTTCATCAAGCTGATCAAGATGATCA
TCGCGCCAATCATCTTCTGCACAGTAGTGGTGGGCATTGCTGGCATGGAAGACATGAAGAAGGTCGGCAAGACCGGCGGG
TTGGCGCTGCTGTACTTCGAGGTCGTGAGCAGTATTGCACTGCTGCTCGGCCTGTTGATCATCAACATCGTCAAGCCCGG
GGCCGGAATGAACGTCGATCCATCCACCCTCGACACGAAAGGGATCGCCGCCTACACCGGACCCGGAAAGATGCAGGGCA
CGGTGGATTTTCTGATGAACGTGATTCCGAACACGGTAGTGGATGCATTCGCAAAAGGTGAAATCCTCCAGGTACTGCTG
TTTGCGGTGATGTTCGGCTTCGCGCTGCACAAGTTCGGCGGCCGCGGCACGCTGGTGTTCGACTTCATCGAGAAATTCTC
GCATGTGCTCTTTACCATCGTCGGCTACATCATGAAAGTCGCGCCACTTGGCGCTTTCGGCGCCATGGCCTTCACTATCG
GCAAGTACGGAGTGAGTTCGCTGCTACAACTCGGCCAGTTGATGGCAACCTTTTACGCCACTTGTCTGGTTTTCATCTTC
GTCGTGCTGGGTACCATTGCGCGACTGCATGGATTTTCGATTTGGAAGTTCATCAAGTACATCAAGGAGGAACTGCTGAT
TGTATTGGGCACATCTTCCTCGGAATCCGTCTTGCCACGGATGATGGCCAAACTGGAGAACTTGGGCGTCCGAAAGTCCG
TCGTCGGACTGGTGATTCCAACTGGCTACTCCTTCAACCTGGACGGTACCTCCATATACCTGACCATGGCGGCTGTGTTC
ATCGCCCAGGCAACGAACACCGACATGAACCTGACGCAGCAACTCACGCTGCTCGCCGTGCTGCTCCTGACATCCAAGGG
GGCCGCTGGGGTCACCGGGAGTGGTTTCATCGTGCTCGCGGCTACGCTATCGGCGGTAGGGCATGTTCCGGTAGCGGGAC
TTGCCTTGATCCTCGGTATTGATCGCTTCATGTCGGAGGCACGCGCCCTGACCAACCTAATTGGCAACGGCGTGGCCACG
GTGGTGGTGGCGAAGTGGACCGGTGACCTCGATCTGGAACGAATGCGTCGCAGACTGGATGACGAGACGGATAGCGAAGC
CGACGAGCCGGAAATGGTGCTCGACCAGACGGCGACCCATATGCCGGCCGTCGACCCTGCGGTAACCCGACGCGGGTAG

Upstream 100 bases:

>100_bases
GGCAGCGATGAAATGCACGGCACCTTAGTCCCTCACTCCTGCTATGTCAGCGGGGCTGATCCGACCCGACTGGCGGTCTG
AACCAGCAATGGAGACGATG

Downstream 100 bases:

>100_bases
CCGCCCAATCACACAAGGCCCCTTCTTGACGACAGGTGACGGCTGCTGAGGCCGTTCAGCCCGCTTCCCTCTCATGGTTA
TTTCAAGGTTATTTGCTTGC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEKVDNRKPLYRSLYFQVIVAIISGVVLGHYYPTAGEAMKPLGDGFIKLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGG
LALLYFEVVSSIALLLGLLIINIVKPGAGMNVDPSTLDTKGIAAYTGPGKMQGTVDFLMNVIPNTVVDAFAKGEILQVLL
FAVMFGFALHKFGGRGTLVFDFIEKFSHVLFTIVGYIMKVAPLGAFGAMAFTIGKYGVSSLLQLGQLMATFYATCLVFIF
VVLGTIARLHGFSIWKFIKYIKEELLIVLGTSSSESVLPRMMAKLENLGVRKSVVGLVIPTGYSFNLDGTSIYLTMAAVF
IAQATNTDMNLTQQLTLLAVLLLTSKGAAGVTGSGFIVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNLIGNGVAT
VVVAKWTGDLDLERMRRRLDDETDSEADEPEMVLDQTATHMPAVDPAVTRRG

Sequences:

>Translated_452_residues
MEKVDNRKPLYRSLYFQVIVAIISGVVLGHYYPTAGEAMKPLGDGFIKLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGG
LALLYFEVVSSIALLLGLLIINIVKPGAGMNVDPSTLDTKGIAAYTGPGKMQGTVDFLMNVIPNTVVDAFAKGEILQVLL
FAVMFGFALHKFGGRGTLVFDFIEKFSHVLFTIVGYIMKVAPLGAFGAMAFTIGKYGVSSLLQLGQLMATFYATCLVFIF
VVLGTIARLHGFSIWKFIKYIKEELLIVLGTSSSESVLPRMMAKLENLGVRKSVVGLVIPTGYSFNLDGTSIYLTMAAVF
IAQATNTDMNLTQQLTLLAVLLLTSKGAAGVTGSGFIVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNLIGNGVAT
VVVAKWTGDLDLERMRRRLDDETDSEADEPEMVLDQTATHMPAVDPAVTRRG
>Mature_452_residues
MEKVDNRKPLYRSLYFQVIVAIISGVVLGHYYPTAGEAMKPLGDGFIKLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGG
LALLYFEVVSSIALLLGLLIINIVKPGAGMNVDPSTLDTKGIAAYTGPGKMQGTVDFLMNVIPNTVVDAFAKGEILQVLL
FAVMFGFALHKFGGRGTLVFDFIEKFSHVLFTIVGYIMKVAPLGAFGAMAFTIGKYGVSSLLQLGQLMATFYATCLVFIF
VVLGTIARLHGFSIWKFIKYIKEELLIVLGTSSSESVLPRMMAKLENLGVRKSVVGLVIPTGYSFNLDGTSIYLTMAAVF
IAQATNTDMNLTQQLTLLAVLLLTSKGAAGVTGSGFIVLAATLSAVGHVPVAGLALILGIDRFMSEARALTNLIGNGVAT
VVVAKWTGDLDLERMRRRLDDETDSEADEPEMVLDQTATHMPAVDPAVTRRG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI21314632, Length=464, Percent_Identity=26.5086206896552, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI5032093, Length=428, Percent_Identity=27.1028037383178, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI40254478, Length=458, Percent_Identity=25.5458515283843, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI169790839, Length=499, Percent_Identity=25.0501002004008, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI223468566, Length=302, Percent_Identity=30.1324503311258, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI223468564, Length=302, Percent_Identity=30.1324503311258, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI4827012, Length=320, Percent_Identity=28.4375, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI66773030, Length=414, Percent_Identity=27.0531400966184, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI194239697, Length=264, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI262359914, Length=424, Percent_Identity=24.5283018867925, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI301601644, Length=226, Percent_Identity=28.3185840707965, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1789947, Length=423, Percent_Identity=70.6855791962175, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1790514, Length=440, Percent_Identity=39.5454545454545, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788024, Length=404, Percent_Identity=23.5148514851485, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789473, Length=335, Percent_Identity=23.8805970149254, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=439, Percent_Identity=24.6013667425968, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=445, Percent_Identity=25.8426966292135, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=429, Percent_Identity=25.8741258741259, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI193206654, Length=444, Percent_Identity=23.1981981981982, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=427, Percent_Identity=23.4192037470726, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=437, Percent_Identity=24.0274599542334, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=431, Percent_Identity=20.4176334106729, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=422, Percent_Identity=23.2227488151659, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137668, Length=422, Percent_Identity=23.2227488151659, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24583023, Length=422, Percent_Identity=23.2227488151659, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281360481, Length=470, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281360483, Length=470, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137666, Length=470, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA2_RALME (Q1LFB1)

Other databases:

- EMBL:   CP000353
- RefSeq:   YP_586434.1
- ProteinModelPortal:   Q1LFB1
- SMR:   Q1LFB1
- STRING:   Q1LFB1
- GeneID:   4041157
- GenomeReviews:   CP000353_GR
- KEGG:   rme:Rmet_4299
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   ACFYITC
- PhylomeDB:   Q1LFB1
- ProtClustDB:   PRK01663
- BioCyc:   RMET266264:RMET_4299-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 48451; Mature: 48451

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1033f2ec)-; HASH(0xb8afdc8)-; HASH(0xf69fccc)-; HASH(0x10c4ab2c)-; HASH(0x10dacb8c)-; HASH(0x108e7e84)-; HASH(0xda9247c)-; HASH(0x10e07638)-; HASH(0x10c956f8)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCHHHHHHHHHCCCCCCCHHCCCH
>Mature Secondary Structure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CCCCCCCCCCHHHHHHHHHCCCCCCCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA