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The map label for this gene is gno [H]

Identifier: 94313124

GI number: 94313124

Start: 782242

End: 783138

Strand: Reverse

Name: gno [H]

Synonym: Rmet_4197

Alternate gene names: 94313124

Gene position: 783138-782242 (Counterclockwise)

Preceding gene: 94313129

Following gene: 94313123

Centisome position: 30.35

GC content: 61.87

Gene sequence:

>897_bases
ATGCATCTTACGATATATATCGTACGATATAAAGTCAAGGACGAATTCTCGGGCGCACGATACGGGAACGTTCCTGTCAT
AATGCAAGCCACTACGCAAACACCCAGGACACCCAAGGCATTCCACGCAGTACCCTTTCCGCAATTCAACCTGGCAGGAG
GAGACACCGTGCCTGAGAAGAAAGCCATCCTCGTCGTCGGCGCCGGCGACGCCACCGGGGGCGCCATCGCCCGCCGCTTT
GCCCACGAGGGTTATGTCGCCTGCGTCACCCGACGCGATGTCGACAAACTCGCGCCACTGGTCGAGCAGATTCGGGCCGA
AGGTGGCGAAGCCCATGCCTTTGGCTGCGACGCGCGCAAGGAAGAAGAGACGGTTGCCATGATCGAGCGCATCGAGCGCG
ATATCGGGCAACTGGAAGTCGCGGTGTTCAACATCGGCGCGAACGTGAATTTCCCGATTCTGGAGACCACCTCGCGCGTC
TACTTCAAGGTCTGGGAGATGGCCTGCTTCGCGGGATTCCTGATGGGCCGCGAAGCCGCGCGCGTCATGGTGCCCCGGCA
GCGCGGGTCGATCTTCTTCACCGGCGCCACCGCAAGCACGCGCGGCCGCGAGGGCATGTCGGCATTCTCGGGCGCGAAGC
ACGCGCTGCGCGCGCTGGCGCAAAGCATGGCACGCGAACTTGGCCCGAAGAACCTCCACGTCGCCCACCTGATCATCGAC
GCCGCCATCGACACCGCCTTCATCCGCGACAATTTCCCGGACCGCTACGCCACCAGGACACAGGATGGCATCGTCAATCC
GGAGCACATCGCGGACACTTACTGGATGCTGCATCAACAGCCACGCGATGCCTGGACCCACGAACTCGATCTGCGTCCCT
GGATCGAACACTGGTAA

Upstream 100 bases:

>100_bases
CACTGTGTCCACCGTGCCGCCCTTGATGACCTTGGCGACCTTCACCCCTACCATCAGAACCAAAGCAGTCCCTCATGATT
GCACTCCACCAAGTCTTACG

Downstream 100 bases:

>100_bases
TCGCCCTCAAGCCAGCATTGCCAAGAAGCACAAAGCCGGACGCAAAGCCGGACACAAAGGAGACATGCAATGAACAAGCA
GGTGGAATTTTTCTTCGATT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 5-keto-D-gluconate 5-reductase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEKKAILVVGAGDATGGAIARRF
AHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARKEEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRV
YFKVWEMACFAGFLMGREAARVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIID
AAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW

Sequences:

>Translated_298_residues
MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEKKAILVVGAGDATGGAIARRF
AHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARKEEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRV
YFKVWEMACFAGFLMGREAARVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIID
AAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW
>Mature_298_residues
MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEKKAILVVGAGDATGGAIARRF
AHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARKEEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRV
YFKVWEMACFAGFLMGREAARVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIID
AAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW

Specific function: Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=174, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI32483357, Length=197, Percent_Identity=27.4111675126904, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790717, Length=237, Percent_Identity=25.3164556962025, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789057, Length=214, Percent_Identity=25.2336448598131, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=168, Percent_Identity=23.2142857142857, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322067, Length=181, Percent_Identity=25.414364640884, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281364892, Length=170, Percent_Identity=27.6470588235294, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640492, Length=204, Percent_Identity=25.4901960784314, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355319, Length=199, Percent_Identity=23.6180904522613, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.69 [H]

Molecular weight: Translated: 33250; Mature: 33250

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEK
CEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCCCCEECCCCCCCCCC
KAILVVGAGDATGGAIARRFAHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARK
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCC
EEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRVYFKVWEMACFAGFLMGREAA
HHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHH
RVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIID
HEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
AAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW
HHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHCCEECCCCHHHCCC
>Mature Secondary Structure
MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEK
CEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCCCCEECCCCCCCCCC
KAILVVGAGDATGGAIARRFAHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARK
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCC
EEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRVYFKVWEMACFAGFLMGREAA
HHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHH
RVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIID
HEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
AAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW
HHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHCCEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751271 [H]