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The map label for this gene is ynfL [H]

Identifier: 94313129

GI number: 94313129

Start: 786646

End: 787533

Strand: Reverse

Name: ynfL [H]

Synonym: Rmet_4202

Alternate gene names: 94313129

Gene position: 787533-786646 (Counterclockwise)

Preceding gene: 291481519

Following gene: 94313124

Centisome position: 30.52

GC content: 65.43

Gene sequence:

>888_bases
ATGGACTTTCGCCAACTTCGCTATTTCGTGGCCGTGGCCGAGGCGCTGAGTTTCAGCGAGGCCGCCCGCCGGCTGCATGT
CAGCCAACCGCCGCTCAGCATCCAGATCAAGGCACTGGAAGATGAACTGGGCAGCCCGCTGCTGGAACGGTCGCGCCATC
ACGTGGCGCTGACGCCGGCCGGAGAACTGTTCCTGGAAAAGGCACGGGCGGCACTGGCCAATCTCGACGAAGCGCGTGAC
GTGGTCAGGCAGGTGGCCTCCGGAGAGGCAGGAGAGATACGCGTGGGGTTTACGGCGTCCGTGCCGATGCAGGATGTCTT
TCCGCGCGCCGTGCACGCCTTCCGCGAGGCACATCGCCATGCCCGGCTCGAACTGGTGCACATGTCCACCGGCCAGCAGC
TCCAGCAACTCGAAAGTGGCGAAATCGACATTGGATTCCTGCGTCCCTCACCGCAGTTCCGGCCACCGGCGCGTATTGAA
GTGCGGGAGGTCCTGGCGGACCGTCTGGTGGCGGTGCTACCGCTCGGCCACCTGTTGGCGCGATCGAACGAGCAGACGGA
GATCGACATCAGCGAACTGTCCGGCGAGAGGTTCCTGCTGTTTCCCCGCGGACTCGGTTGCGGCCTGTTCGATCACGTGA
CCACACTGTGCAATCGCGCCGGATTCGCGCCGAATGTCGCGCAGGAAGCGCGCGAGGCCACCACGATCATCGGCCTGGTA
GCCAGTGGTGCCGGGGTGTCGGTGCTACCGGCGATATACGCGCGCACGGGCATCCCCGGCGTGGCGTACCGCGCCATCGC
CGGGCCCGACGCAGGCAGCCGCATCCTGATGGCGCATCGCGCGGATGCGCTATCGCCGATCATGAAACGGTTCGTGGGAT
ATTTCTGA

Upstream 100 bases:

>100_bases
GATGTCTCCAGATGTTATTGATCTCCTGGCACATTCTCGGCGCGGGCCGTGATACTGTCCAATATGTTTTCAGGTGGCTC
GTTATTTCGAATTCGTATCA

Downstream 100 bases:

>100_bases
GGTTGGGAGTTGCCGATTGCTGGCTCCCCTCTTCCGCTCGCGGAAGAGGGGAAAAAACCAACTGCAGATCAGCCGCCTGG
CGCCGTCACCAGCCCATCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPAGELFLEKARAALANLDEARD
VVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRHARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIE
VREVLADRLVAVLPLGHLLARSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLV
ASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF

Sequences:

>Translated_295_residues
MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPAGELFLEKARAALANLDEARD
VVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRHARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIE
VREVLADRLVAVLPLGHLLARSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLV
ASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF
>Mature_295_residues
MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPAGELFLEKARAALANLDEARD
VVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRHARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIE
VREVLADRLVAVLPLGHLLARSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLV
ASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=277, Percent_Identity=38.9891696750903, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=34.8122866894198, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=31.3207547169811, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1790399, Length=301, Percent_Identity=30.8970099667774, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI157672245, Length=254, Percent_Identity=31.8897637795276, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=298, Percent_Identity=25.1677852348993, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788297, Length=257, Percent_Identity=26.4591439688716, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=28.7937743190661, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787601, Length=250, Percent_Identity=28.8, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=289, Percent_Identity=25.6055363321799, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=250, Percent_Identity=26.4, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=33.1081081081081, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=26.2745098039216, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693193, Length=120, Percent_Identity=34.1666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=302, Percent_Identity=26.4900662251656, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32178; Mature: 32178

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCHHHHCCCCEEEECCH
GELFLEKARAALANLDEARDVVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRH
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
ARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIEVREVLADRLVAVLPLGHLLA
HHEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLV
CCCCCCEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF
CCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCHHHHCCCCEEEECCH
GELFLEKARAALANLDEARDVVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRH
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
ARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIEVREVLADRLVAVLPLGHLLA
HHEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLV
CCCCCCEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF
CCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]