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The map label for this gene is nemA [H]

Identifier: 94313030

GI number: 94313030

Start: 671239

End: 672348

Strand: Direct

Name: nemA [H]

Synonym: Rmet_4103

Alternate gene names: 94313030

Gene position: 671239-672348 (Clockwise)

Preceding gene: 94313029

Following gene: 94313032

Centisome position: 26.02

GC content: 64.14

Gene sequence:

>1110_bases
ATGGGTGCACAGACCGAAGCATTCCTCACCGAAGCCCTGTTTCAGCCGATCAAGCTGGGCCGTATCGAACTCGCCAACCG
CATGGCCATGGCACCGCTCACGCGCAGCCGCGCCGATGACGATCTGGTGCCGACCGAGATGGTGGTCGAGTACTACCGCC
AGCGCGCCAGCGTCGGCCTGATCATCGCCGAAGCCACGCAGGTTTCGGAGACTGCCCAGGGCTACACGAACACCCCCGGC
GTCTATACGCCCGAGCAGATCGCGGCCTGGAAGACGGTGACCGACGCCGTGCACGCGCAAGGCGGCAAGATTTTCCTGCA
GATCTGGCACACGGGTCGCATGTCGCACTCGCATTTCCAGCCGGACAATCAGGCACCGGTGGCGCCTTCGGCCATTGCGG
CCAATGCCAAGACGTATATCAACGGCCAGGGCTATGTCGAATGCTCGCTGCCGCGCGCGCTGGAAGCTTCGGAGATTCCG
GAGATCGTGAATGACTTCCGCGTCGCCGCGGCCAACGCGATCAAGGCTGGCTTCGATGGCGTCGAAGTGCATGGCGCGCA
CGGCTATCTGCTCGATGCCTTCCTGCGCGATGGCACGAACAAGCGTACCGACGCCTACGGCGGCAGCATCGAGAACCGCG
CCCGCTTCCTGCTTGAAGTCATGGCGGCGGTGACTGCCGAAATCGGTGCCGATCGCGTGGGTGTCCGACTGGCGCCGGTG
TCGCCGGTCAACGACGCGGTGGAAAGCAATCCGCAGCCGCTGTTCGAGTACGTCGTGCGTGAGCTGGAAAAACTCCATCC
GGTGTACATCCACGTGGTCGAAGGCTACACGGGTGGCCCGCGCGACAATGTGCCGTTCGACTACGAGGCGCTGCACCGCC
TGTACTCGGGCGTGTGGATGGTCAATAACGGCTACAGCAAGGAAATGGCCGAGGAAGCGATCCGCGCCGGCCGTGCCGAC
ATGGTCTCGTTCGGCCGCAAGATGATCACCAACCCCGACCTGCCGCGCCGCTTCCGTGAGAACCAGCCGCTGAACAGTCC
GTTCGAGGACGCGTCGCTCTACGGCGGTACTGGCCCGCACGGCTACGTCGACTATCCGGCATTGGCCTGA

Upstream 100 bases:

>100_bases
GCCCCGCTGCGCCGCAGCGTCGACCTGATGTTCGACCACCTGTTCCAGCCGGCCGCCGCGGCCGGCATCCACCACAGTCC
AACCTTGGAGTAAGTCATTC

Downstream 100 bases:

>100_bases
TCTTCGGCCTCGCGCGCTCTACGGGCGGGTTTGCATCCGGACCTATGTCCGGATGTCCATAGGTCCTTACGTCCTTAGAG
GCGCTATCAAAATGAAGCGA

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MGAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGLIIAEATQVSETAQGYTNTPG
VYTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQPDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIP
EIVNDFRVAAANAIKAGFDGVEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPV
SPVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWMVNNGYSKEMAEEAIRAGRAD
MVSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPHGYVDYPALA

Sequences:

>Translated_369_residues
MGAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGLIIAEATQVSETAQGYTNTPG
VYTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQPDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIP
EIVNDFRVAAANAIKAGFDGVEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPV
SPVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWMVNNGYSKEMAEEAIRAGRAD
MVSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPHGYVDYPALA
>Mature_368_residues
GAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGLIIAEATQVSETAQGYTNTPGV
YTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQPDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIPE
IVNDFRVAAANAIKAGFDGVEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPVS
PVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWMVNNGYSKEMAEEAIRAGRADM
VSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPHGYVDYPALA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=49.1803278688525, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789463, Length=237, Percent_Identity=35.8649789029536, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=331, Percent_Identity=26.2839879154079, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=259, Percent_Identity=29.7297297297297, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=253, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=336, Percent_Identity=26.4880952380952, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=210, Percent_Identity=30, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=221, Percent_Identity=31.2217194570136, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=368, Percent_Identity=23.6413043478261, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=384, Percent_Identity=36.1979166666667, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=383, Percent_Identity=34.7258485639687, Blast_Score=190, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40471; Mature: 40340

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHCCEEE
IIAEATQVSETAQGYTNTPGVYTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQ
EEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
PDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIPEIVNDFRVAAANAIKAGFDG
CCCCCCCCCHHHHCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
VEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPV
EEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
SPVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWM
CCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCEEE
VNNGYSKEMAEEAIRAGRADMVSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPH
EECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
GYVDYPALA
CCCCCCCCC
>Mature Secondary Structure 
GAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHCCEEE
IIAEATQVSETAQGYTNTPGVYTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQ
EEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
PDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIPEIVNDFRVAAANAIKAGFDG
CCCCCCCCCHHHHCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
VEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPV
EEEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
SPVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWM
CCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCEEE
VNNGYSKEMAEEAIRAGRADMVSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPH
EECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
GYVDYPALA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]