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The map label for this gene is selU [H]

Identifier: 94312787

GI number: 94312787

Start: 401419

End: 402480

Strand: Reverse

Name: selU [H]

Synonym: Rmet_3859

Alternate gene names: 94312787

Gene position: 402480-401419 (Counterclockwise)

Preceding gene: 94312788

Following gene: 94312786

Centisome position: 15.6

GC content: 65.54

Gene sequence:

>1062_bases
ATGAAACCAGTCGTAAGAGTCGAAGACCGCCACGCGTTCGACACGATCATCGACGCCCGCTCACCGGCGGAGTTCGAGAT
CGACCATATTCCCGGCGCCATCAACTGCCCGGTTCTCGATAACGAGGAGCGCCGCATCGTCGGCACGCTGTACAAGCAGG
TCGGCGCATTCGAGGCGCGACGTGTCGGCGGTGCCATGGTGGCAGCCAACCTCGCCCGCCATTTGCGCGAGCAGTTCGCC
GATCGCCCGTCTGGCTGGCGGCCGCTGGTGTATTGCTGGCGCGGCGGGCTGCGCAGCGGCTCGATGACGACGTGGATGCG
CATGGTGGGCTGGGATGCCCAGCAGCTTGCCGGCGGCTACAAGGCCTGGCGCCGTCACGTTATCGATGTGCTCGACACGC
AGGCCGCGCGCGCGCCGCTGGTCGTGATCTGCGGCGCCACAGGCAGCGGCAAGACGCGCGTGCTCCATGCACTGGCCGCA
CAAGGCGAACAGGTGCTCGATCTCGAAGGGCTGGCCCGGCACAAGGGATCGCTGCTCGGCGCATTGCCCGATGTCGCCCA
GCCTTCACAGACCGCGTTCGAGACCGCACTGGCGGATGTGATCGAGCGCGTCGATCTGGCCCGGCCGCTTTATGTGGAAG
GTGAAGGCGCGCGCATTGGCAAGCTCAATCTGCCGGTGCCGCTGGTTGCCCACATGCGCGAAAGCGCCTGCGTGGAGATC
GACGCATCGCCAGAAACACGACTCGCGTTCCTGCTGCATGACTATGCGTATCTTGGCGATCGGCCCGCCTGGCTTGCCGA
TCAGCTTGGGCGGCTGAAGGAACTGCACGGCAAGACCATCATCGCCGCGTGGCAGGAGATGGCGACCACAGGAAACCTGC
GCCAGCTGTTCGGCGAACTGGCGACCGAGCACTACGACCCTTCCTACGCGCGCTCGCAACGCAAGCATTTTCACAAATGG
GACGCGCGGCGCGTGGTGCACGCGGACAGCCTCTCCGCGCAGGGCATAGAAGCGTTGGCTAGGTCGATCGTGCAAACAGT
GGCGGAGCAGCAACCCGCCTGA

Upstream 100 bases:

>100_bases
CCGTGGGCAAGGTACTGGCCGTCTTCCGTGAGCAGGGCTTCACGGAAGCCACCGTGGTGGGCCGCATGGAAGCCGGCGAA
CCCGGGCTGACGGTCACCCC

Downstream 100 bases:

>100_bases
GTCCAGTCGCTACGCGTCTCATACGGACGGGTAGCGTCGACATGCGGAATTCGCGCTTAGTGACGCGGGCGGGATTGGTT
ACTCTGAACGGCCCAGAGCA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEARRVGGAMVAANLARHLREQFA
DRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGYKAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAA
QGEQVLDLEGLARHKGSLLGALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEI
DASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGELATEHYDPSYARSQRKHFHKW
DARRVVHADSLSAQGIEALARSIVQTVAEQQPA

Sequences:

>Translated_353_residues
MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEARRVGGAMVAANLARHLREQFA
DRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGYKAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAA
QGEQVLDLEGLARHKGSLLGALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEI
DASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGELATEHYDPSYARSQRKHFHKW
DARRVVHADSLSAQGIEALARSIVQTVAEQQPA
>Mature_353_residues
MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEARRVGGAMVAANLARHLREQFA
DRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGYKAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAA
QGEQVLDLEGLARHKGSLLGALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEI
DASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGELATEHYDPSYARSQRKHFHKW
DARRVVHADSLSAQGIEALARSIVQTVAEQQPA

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=332, Percent_Identity=34.6385542168675, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 39022; Mature: 39022

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEAR
CCCCEEECCCHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHH
RVGGAMVAANLARHLREQFADRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCHHHHHHHH
KAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAAQGEQVLDLEGLARHKGSLLG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEECHHHHHHHCCCHHH
ALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHCCCCCEEEE
DASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGEL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
ATEHYDPSYARSQRKHFHKWDARRVVHADSLSAQGIEALARSIVQTVAEQQPA
HHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEAR
CCCCEEECCCHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHH
RVGGAMVAANLARHLREQFADRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCHHHHHHHHCCCHHHHHHHH
KAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAAQGEQVLDLEGLARHKGSLLG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEECHHHHHHHCCCHHH
ALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHCCCCCEEEE
DASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGEL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
ATEHYDPSYARSQRKHFHKWDARRVVHADSLSAQGIEALARSIVQTVAEQQPA
HHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]