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The map label for this gene is dppA [H]

Identifier: 94312089

GI number: 94312089

Start: 3422107

End: 3423714

Strand: Reverse

Name: dppA [H]

Synonym: Rmet_3158

Alternate gene names: 94312089

Gene position: 3423714-3422107 (Counterclockwise)

Preceding gene: 94312092

Following gene: 94312088

Centisome position: 87.16

GC content: 60.76

Gene sequence:

>1608_bases
ATGTCCCTTCGTCACATCAGGAAGGCCATCGGCAAGACGATCAACACCGTCCTGCTGACCAGCGCCCTCGGCCTCGGTTT
GGCCGCGCCAGTTCACGCCGTGGATCTGAAGATCGCCATGAGTTCGCCACCGACCTCGATGGATCCACATTTCTACAACC
TTTTCTCGAACATCAACGTGTCGGAGCACGTGTTCGAGACCCTGATCAAGCTTGATCCGGACAGCCGCGTCATTCCTGGA
CTTGCCGAGTCGTGGAAGCTCGTCAACAACCTGACCTGGGAATTCAAGATCCGCAAGGGCGTCAAGTGGCATGACGGCAC
GGAGCTGACCACCGAGGACATCGCCTGGTCACTGGACCGGCCTGCCACGATCCTCAACAGCCCGGGCAAGTTCGATGTGT
ACACCAAGGCGATCATCAACAAGAAGATCATCGACAAGTACACGATCCAGCTGACCACCAACCAGCCGTATCCGTTGATG
CTCAACGACCTGACGGCGGTGTTCATGGTGCAGAAGAAGGCCACGCAGGGCCTGTCTTCCGACGACTTCGCGCAGGGCAA
GGGCATGATCGGCACCGGGCCGTTCAAGTTCGTCAGCTACGCGCGCGACGATCGCGTCGAGCTGGTCAGGAACCCCGACT
ACTGGGGGCCGAAGCCGGCCTGGGATAAGGTGACGCTGCGCTTCATCCCGAACCCGGCCACGCGTCTGGCGGCGCTGCTG
TCGGGCGATGTGCAGGCGATCGAGAACGTGCCGACGCCGGACCTGCCCAAAGTCCGCAACGATCCCAAGCTTTCGTTCTT
CTCGAAGATCTCGCATCGCGTGATCTATCTGTACTTCGACGCCAAACGGGACAAGTCACCGTTCGTGACCACGAAGGACG
GCCAGGCGATGGACAAGAATCCGCTCAAGGACCCGCGCGTGCGTCTTGCCATCAGCACGGCCATCAATCGCCAGGGCATC
AAGGATCGTCTGATGGAGGGGCTGGCGGAGCCAACCAATAACCTCGTGCCGCCGACGATGTTCGGCTACAACCCGGCGCT
GAAGACGATCAAGTACGACCCCGAGGCGGCGAAGAAGATGCTAGCCGAGGCGGGCTACCCCAATGGCTTCGGGTTGACGT
TGCATACGCCGAACAACCGGTATGTCAACGACGAGAAGATCGCGCAGACCATCGCGCAGAACCTGACGCGCATTGGCATC
AACACCAAGGTGGAAGGGATGCCGATGGCCACCTACTCGGCGAAGGGGATCAAGCACGAATGGTCGTTCGGCCTGCTGGG
CTGGGGCGCCCAGACCGGTGAGGTCAGCTCGCCGCTGCGCGCGCTGCTAGCCTGCGAGGACTCCAAGAAGGGCTTCGGTA
CCACCAACTGGGGCGAGTACTGCAATCCGAAGATGGATGTGGTGCTGGAGAAGGCACTGGCCACCGTGGATGACAATGAG
CGCTCGAAGCTTCTGCAGGAAGCCACGGCTATCGCCATCCTTGATGGTGGCATCATTCCGATCCATCACCAGGTGACCAC
GTGGGCCACGCAGAAGGGCATCGTGTACATCCCCCGTACCGACGAACGGACATACGCGCACAACTTCAAGCCGGCCGGCG
CGCAATGA

Upstream 100 bases:

>100_bases
CAGAATCGTCGCACCAAAATGCGGCCTTCCTCACGGGCAATCGACCGACGTGGCTGATCCTAACCGTCATTTCAAGACTT
CCCCCTGGGAGAATCGCGCG

Downstream 100 bases:

>100_bases
TTCTGCCTGCACGCGGGCCTTTGATGGCTCCGGTATGATCGCGGCCCCGACTGCGTCCATGCAGCCGGGGCAATCCGCCC
ACGCCTTCCCCCGTGGGCCG

Product: ABC-type dipeptide transport system periplasmic protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSLRHIRKAIGKTINTVLLTSALGLGLAAPVHAVDLKIAMSSPPTSMDPHFYNLFSNINVSEHVFETLIKLDPDSRVIPG
LAESWKLVNNLTWEFKIRKGVKWHDGTELTTEDIAWSLDRPATILNSPGKFDVYTKAIINKKIIDKYTIQLTTNQPYPLM
LNDLTAVFMVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALL
SGDVQAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPFVTTKDGQAMDKNPLKDPRVRLAISTAINRQGI
KDRLMEGLAEPTNNLVPPTMFGYNPALKTIKYDPEAAKKMLAEAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQNLTRIGI
NTKVEGMPMATYSAKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNE
RSKLLQEATAIAILDGGIIPIHHQVTTWATQKGIVYIPRTDERTYAHNFKPAGAQ

Sequences:

>Translated_535_residues
MSLRHIRKAIGKTINTVLLTSALGLGLAAPVHAVDLKIAMSSPPTSMDPHFYNLFSNINVSEHVFETLIKLDPDSRVIPG
LAESWKLVNNLTWEFKIRKGVKWHDGTELTTEDIAWSLDRPATILNSPGKFDVYTKAIINKKIIDKYTIQLTTNQPYPLM
LNDLTAVFMVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALL
SGDVQAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPFVTTKDGQAMDKNPLKDPRVRLAISTAINRQGI
KDRLMEGLAEPTNNLVPPTMFGYNPALKTIKYDPEAAKKMLAEAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQNLTRIGI
NTKVEGMPMATYSAKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNE
RSKLLQEATAIAILDGGIIPIHHQVTTWATQKGIVYIPRTDERTYAHNFKPAGAQ
>Mature_534_residues
SLRHIRKAIGKTINTVLLTSALGLGLAAPVHAVDLKIAMSSPPTSMDPHFYNLFSNINVSEHVFETLIKLDPDSRVIPGL
AESWKLVNNLTWEFKIRKGVKWHDGTELTTEDIAWSLDRPATILNSPGKFDVYTKAIINKKIIDKYTIQLTTNQPYPLML
NDLTAVFMVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLS
GDVQAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPFVTTKDGQAMDKNPLKDPRVRLAISTAINRQGIK
DRLMEGLAEPTNNLVPPTMFGYNPALKTIKYDPEAAKKMLAEAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQNLTRIGIN
TKVEGMPMATYSAKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNER
SKLLQEATAIAILDGGIIPIHHQVTTWATQKGIVYIPRTDERTYAHNFKPAGAQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=517, Percent_Identity=26.1121856866538, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787052, Length=494, Percent_Identity=28.5425101214575, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787762, Length=504, Percent_Identity=25.1984126984127, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787551, Length=487, Percent_Identity=24.0246406570842, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787495, Length=495, Percent_Identity=24.8484848484848, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081878, Length=521, Percent_Identity=24.5681381957774, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789887, Length=462, Percent_Identity=25.3246753246753, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789397, Length=533, Percent_Identity=23.827392120075, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59476; Mature: 59345

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRHIRKAIGKTINTVLLTSALGLGLAAPVHAVDLKIAMSSPPTSMDPHFYNLFSNINV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHCCCH
SEHVFETLIKLDPDSRVIPGLAESWKLVNNLTWEFKIRKGVKWHDGTELTTEDIAWSLDR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHEECCCC
PATILNSPGKFDVYTKAIINKKIIDKYTIQLTTNQPYPLMLNDLTAVFMVQKKATQGLSS
CHHHHCCCCCEEHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCC
DDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALL
CCHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
SGDVQAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPFVTTKDGQAMDKN
HCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEECCCCCCCCCC
PLKDPRVRLAISTAINRQGIKDRLMEGLAEPTNNLVPPTMFGYNPALKTIKYDPEAAKKM
CCCCCCEEEEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEECHHHHHHH
LAEAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQNLTRIGINTKVEGMPMATYSAKGIKHE
HHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCEECCCEEEEECCCCCCCC
WSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNE
CCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCH
RSKLLQEATAIAILDGGIIPIHHQVTTWATQKGIVYIPRTDERTYAHNFKPAGAQ
HHHHHHHHHEEEEECCCEEEEECEEEEEECCCCEEEEECCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
SLRHIRKAIGKTINTVLLTSALGLGLAAPVHAVDLKIAMSSPPTSMDPHFYNLFSNINV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHCCCH
SEHVFETLIKLDPDSRVIPGLAESWKLVNNLTWEFKIRKGVKWHDGTELTTEDIAWSLDR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHEECCCC
PATILNSPGKFDVYTKAIINKKIIDKYTIQLTTNQPYPLMLNDLTAVFMVQKKATQGLSS
CHHHHCCCCCEEHHHHHHHHHHHCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCC
DDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALL
CCHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
SGDVQAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPFVTTKDGQAMDKN
HCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEECCCCCCCCCC
PLKDPRVRLAISTAINRQGIKDRLMEGLAEPTNNLVPPTMFGYNPALKTIKYDPEAAKKM
CCCCCCEEEEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEECHHHHHHH
LAEAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQNLTRIGINTKVEGMPMATYSAKGIKHE
HHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCEECCCEEEEECCCCCCCC
WSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNE
CCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCH
RSKLLQEATAIAILDGGIIPIHHQVTTWATQKGIVYIPRTDERTYAHNFKPAGAQ
HHHHHHHHHEEEEECCCEEEEECEEEEEECCCCEEEEECCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]