Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 94311875

GI number: 94311875

Start: 3199089

End: 3200150

Strand: Direct

Name: queA [H]

Synonym: Rmet_2943

Alternate gene names: 94311875

Gene position: 3199089-3200150 (Clockwise)

Preceding gene: 94311870

Following gene: 94311876

Centisome position: 81.44

GC content: 68.27

Gene sequence:

>1062_bases
ATGTTGACCCTGTCCGATTTCGATTTTCCGCTGCCGCCCGAACTGATCGCGCAGTCCGCCCTGCCTGACCGCAGCGCCAG
CCGTCTGCTGGTGGTGGAGCGGACCGCCCCCGAGGACACGTCCGAGGCGGTGCGGCTGGTCGACCGCGCCTTCAGCGACA
TCCTTGAATATCTGAATCCGGACGACCTGCTCGTGTTCAACGACACGCGCGTGATCAAGGCCCGTTTTTTCGGCCACAAG
CCGAGCGGCGGCCGGATCGAAGTGCTGGTGGAACGGGTGGTCGATACCCACACGGTGCTGGCCCAGGTGCGCGCGTCGAA
GACGCCTGTCGAGGGCAGCCTGCTGCACCTGGCGGATGACGCCTTCGCAGTGACCGTGGGCCCCCGCGTCGACCAGTTCT
TCACACTGCGCTTTCCCGAGCCCGCGCTCGACCTGATCGAACGTTACGGCCGCCTGCCGCTGCCGCCGTACATCACCCAC
GACCCCGACGCCTACGACGAAACGCGCTACCAGACCGTCTACGCGCGCAACCCCGGCGCGGTGGCCGCGCCCACGGCCGG
ATTGCACTTCGACGACGCCCTGTTCGCCCGGCTGGACGCCGCCGGCATCCGCCGCGCCTTCCTGACGCTGCACGTTGGCG
CGGGCACGTTCCAGCCCGTGCGTACCGAAAACCTGTCCGAGCACAAGATGCATTCGGAGTGGTATGCCATCTCGCCCGAA
CTGGCGGATGCCGTGCGCGAGACCCGCGCGCGTGGCGGACGCGTGATCGCCGTCGGCACCACGTCGCTGCGCGCGCTGGA
ATCGGCCGCCGCCGAAGATGGCACGCTGGAGGCGGGCAGCGGCGATACTGACATCTTCATCACGCCCGGCTACGCCTTCC
GCATCGTCGACGCGCTGATCACCAACTTCCATCTGCCCAAGTCCACGCTGCTGATGCTGGTCTCCGCGCTGGCCGGCGTG
GAGGCGATTCGCGACGCCTACCGTCATGCCGTGGACGCGCGCTACCGCTTCTTCAGCTACGGCGACGCCATGCTGCTCAC
CCGCCGCGACCGCCGCGCCTGA

Upstream 100 bases:

>100_bases
AATGTCGGATTCGCCGATTTTACCCATGCCGTACCGATCACCGCGGTAGCACGCCCACAAAGCTTGCCGCCGTGCACGGT
TTTGGCCGTTGTATCCAGCC

Downstream 100 bases:

>100_bases
CCGCCCCCGAGAACACACCATGCTCAATTTCGAACTGCTGACCACTGATGGCAACGCCCGCCGTGGCCGCGTCACGCTGA
ACCACGGCGTTGTGGAAACG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNPDDLLVFNDTRVIKARFFGHK
PSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADDAFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPE
LADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALITNFHLPKSTLLMLVSALAGV
EAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA

Sequences:

>Translated_353_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNPDDLLVFNDTRVIKARFFGHK
PSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADDAFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPE
LADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALITNFHLPKSTLLMLVSALAGV
EAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA
>Mature_353_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNPDDLLVFNDTRVIKARFFGHK
PSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADDAFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPE
LADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALITNFHLPKSTLLMLVSALAGV
EAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=351, Percent_Identity=51.8518518518518, Blast_Score=343, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39036; Mature: 39036

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNP
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DDLLVFNDTRVIKARFFGHKPSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADD
CCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
AFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITHDPDAYDETRYQTVYARNPGA
CEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
VAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPE
CCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHCCCCEEECHH
LADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALI
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHCCCCCEECCCCCCEEEECCCHHHHHHHHHH
TNFHLPKSTLLMLVSALAGVEAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
>Mature Secondary Structure
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNP
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DDLLVFNDTRVIKARFFGHKPSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADD
CCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
AFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITHDPDAYDETRYQTVYARNPGA
CEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
VAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPE
CCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHCCCCEEECHH
LADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALI
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHCCCCCEECCCCCCEEEECCCHHHHHHHHHH
TNFHLPKSTLLMLVSALAGVEAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA