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The map label for this gene is tgt [H]

Identifier: 94311876

GI number: 94311876

Start: 3200170

End: 3201300

Strand: Direct

Name: tgt [H]

Synonym: Rmet_2944

Alternate gene names: 94311876

Gene position: 3200170-3201300 (Clockwise)

Preceding gene: 94311875

Following gene: 94311877

Centisome position: 81.47

GC content: 63.84

Gene sequence:

>1131_bases
ATGCTCAATTTCGAACTGCTGACCACTGATGGCAACGCCCGCCGTGGCCGCGTCACGCTGAACCACGGCGTTGTGGAAAC
GCCGATCTTCATGCCCGTAGGCACCTACGGGTCGGTCAAGGCAATGTCGCCGCTCGAACTCAACGAGATCGGCGCGCAAA
TCATCCTCGGCAATACGTTCCACCTGTGGCTGCGCCCGGGGCTCGACGTCGTTGACACCCACGCCGGCCTGCACAAGTTC
ATCGGCTGGGACAAGCCGATCCTGACCGATTCGGGCGGTTTTCAGGTGTTTTCGCTGGGAGAACTGCGCAAGATCACCGA
GGAAGGTGTCACGTTCGCATCGCCGGTCAATGGCGACAAGCTGTTCCTGTCGCCCGAGATCTCGATGCAGATCCAGCGCA
CGCTGAACTCAGACATCGTGATGCAATTCGACGAGTGCACGCCGTACGAGATCGACGGCCGTCCCGCCACGCACGACGAG
GCCGCCCGCTCCATGCGGATGAGCCTGCGCTGGGCCAAGCGTTCGCGCGACGAGTTCGACCATCTGGCCAATCCCAACGC
GCTGTTCGGCATCGTGCAGGGCGGCATGTTCGAGGACCTGCGCGACGAATCACTGGCGGGCCTGTCGGAACTGGACTTCC
ATGGTTACGCGATCGGCGGCCTGTCGGTTGGTGAGCCGAAGGAAGACATGATGCGGGTGCTGGAGCACGTTGGTCCCCGC
TTGCCGGCGCACAAGCCGCACTATCTGATGGGCGTTGGCACGCCCGAGGACCTGGTGGCCGGCGTGGCGCACGGCGTCGA
CATGTTCGACTGCGTGATGCCGACCCGCAACGCCCGCAACGGCTGGCTGTTCACGCGCTTTGGTGACGTGAAGATCCGCA
ACGCGGCGCACCGCAACGACCCGCGCCCGCTGGACGAACAGTGCGGCTGCTACACCTGCCGTAACTTCTCCCGGGCCTAC
CTGCACCACCTGCACCGCGTGGGCGAAATCCTTGGCGCGCGCCTGAACACGATCCACAACCTCTATTACTACCTGGAACT
GATGCGCGAGATGCGCACGGCCATCGAAGAGCACCGCTTCGAGGCATTCCGCCGCCAGTTCGCCGAAAATCGCGCGCGCG
GCACACGCTGA

Upstream 100 bases:

>100_bases
CGTCATGCCGTGGACGCGCGCTACCGCTTCTTCAGCTACGGCGACGCCATGCTGCTCACCCGCCGCGACCGCCGCGCCTG
ACCGCCCCCGAGAACACACC

Downstream 100 bases:

>100_bases
GTGTCTCTCGGACCGGGCCGGCTGAGAACTTTCGGCGCGGCCAAGGTCGAATAGCTGGCCTGAAATCGGCCGCTTCGGGG
TGGCTTTCCGCATCCTCCGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLNFELLTTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAQIILGNTFHLWLRPGLDVVDTHAGLHKF
IGWDKPILTDSGGFQVFSLGELRKITEEGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIDGRPATHDE
AARSMRMSLRWAKRSRDEFDHLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGYAIGGLSVGEPKEDMMRVLEHVGPR
LPAHKPHYLMGVGTPEDLVAGVAHGVDMFDCVMPTRNARNGWLFTRFGDVKIRNAAHRNDPRPLDEQCGCYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLYYYLELMREMRTAIEEHRFEAFRRQFAENRARGTR

Sequences:

>Translated_376_residues
MLNFELLTTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAQIILGNTFHLWLRPGLDVVDTHAGLHKF
IGWDKPILTDSGGFQVFSLGELRKITEEGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIDGRPATHDE
AARSMRMSLRWAKRSRDEFDHLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGYAIGGLSVGEPKEDMMRVLEHVGPR
LPAHKPHYLMGVGTPEDLVAGVAHGVDMFDCVMPTRNARNGWLFTRFGDVKIRNAAHRNDPRPLDEQCGCYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLYYYLELMREMRTAIEEHRFEAFRRQFAENRARGTR
>Mature_376_residues
MLNFELLTTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAQIILGNTFHLWLRPGLDVVDTHAGLHKF
IGWDKPILTDSGGFQVFSLGELRKITEEGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIDGRPATHDE
AARSMRMSLRWAKRSRDEFDHLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGYAIGGLSVGEPKEDMMRVLEHVGPR
LPAHKPHYLMGVGTPEDLVAGVAHGVDMFDCVMPTRNARNGWLFTRFGDVKIRNAAHRNDPRPLDEQCGCYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLYYYLELMREMRTAIEEHRFEAFRRQFAENRARGTR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=44.5070422535211, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=66.1290322580645, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=42.1195652173913, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=28.6585365853659, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=43.6974789915966, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI21355311, Length=155, Percent_Identity=29.6774193548387, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42536; Mature: 42536

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFELLTTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAQIILGNTF
CCEEEEEECCCCCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEECCEE
HLWLRPGLDVVDTHAGLHKFIGWDKPILTDSGGFQVFSLGELRKITEEGVTFASPVNGDK
EEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCCCE
LFLSPEISMQIQRTLNSDIVMQFDECTPYEIDGRPATHDEAARSMRMSLRWAKRSRDEFD
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGYAIGGLSVGEPKEDMMRVLEHVGPR
HHCCCCCEEEECCCCHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCC
LPAHKPHYLMGVGTPEDLVAGVAHGVDMFDCVMPTRNARNGWLFTRFGDVKIRNAAHRND
CCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCC
PRPLDEQCGCYTCRNFSRAYLHHLHRVGEILGARLNTIHNLYYYLELMREMRTAIEEHRF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFRRQFAENRARGTR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNFELLTTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAQIILGNTF
CCEEEEEECCCCCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEECCEE
HLWLRPGLDVVDTHAGLHKFIGWDKPILTDSGGFQVFSLGELRKITEEGVTFASPVNGDK
EEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHCCCEEECCCCCCE
LFLSPEISMQIQRTLNSDIVMQFDECTPYEIDGRPATHDEAARSMRMSLRWAKRSRDEFD
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGYAIGGLSVGEPKEDMMRVLEHVGPR
HHCCCCCEEEECCCCHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCC
LPAHKPHYLMGVGTPEDLVAGVAHGVDMFDCVMPTRNARNGWLFTRFGDVKIRNAAHRND
CCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCC
PRPLDEQCGCYTCRNFSRAYLHHLHRVGEILGARLNTIHNLYYYLELMREMRTAIEEHRF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFRRQFAENRARGTR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA