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The map label for this gene is pyrX [H]

Identifier: 94311671

GI number: 94311671

Start: 2974891

End: 2976210

Strand: Reverse

Name: pyrX [H]

Synonym: Rmet_2739

Alternate gene names: 94311671

Gene position: 2976210-2974891 (Counterclockwise)

Preceding gene: 94311672

Following gene: 94311670

Centisome position: 75.77

GC content: 65.98

Gene sequence:

>1320_bases
TTGAGCCGGACCCCGGATACAACGAACAGCACACACCGACAGAACATGAAGATCCATATCCAGGGTGGCCGCCTGATCGA
CCCGGCCGCGAACGTCGACGCCGAACAGGACCTGTACATCGCCGCCGGCAAGATTGTGGGCGTGGGCCGCGCGCCCACTG
ACTTCCATGCCAACAAGACCATCGACGCACGCGGCATGATCGTGTGCCCGGGGCTGGTCGACCTGTCCGCCCGCCTGCGC
GAGCCGGGCTACGAATACAAGGCGACGCTGGAATCCGAACTGGCCGCCGCCACTGCCGGTGGCGTGACCAGCCTGGTCTG
CCCGCCTGACACCGACCCGGTACTCGATGAGCCGGGGCTGGTGGAGATGCTCAAGTTCCGCGCTCACAAGCTCAACCAGA
CGCATGTGTACCCGCTGGGTGCCCTGACCGTCGGCCTGAAGGGCGACGCGCTGACCGAGATGGGCCAGTTGACGGAGGCA
GGCTGTGTCGGCTTCAGCCAGGCCGAGGCGCCCATCCATGACACCAAGGTGCTCCAGCGCGCGATGCAGTACGCGCAGAC
GTTCGGCTTCACGCTGTGGCTGCGTCCAGAGGATCCATTCCTCGGCGGAGGCGTTGCCGCGAGTGGTGCCGTGGCGTCGC
GCCTGGGTCTGTCGGGCGTGTCCGTCATCGCCGAAACCGTGCGTCTGCACACGATCTTCGAATTGATACGTGCCACTGGC
GCACGCGTGCACCTGTGCCGCTTGTCGTCGGCTGCCGGGCTGGAACTCGTGCGTCAGGCACGCAAGGAAGGCCTGCCGGT
CACGTGCGACGTGAACATCCATCACGTGTCGCTGACCGACATGGATATCGGCTTCTTCAATAGCCAGATGCGCTTCACGC
CGCCGCTGCGCGGCGCTCGCGATCGCGACGCAATCGTTGCCGCGCTGGCCGACGGCACCATCGACGCACTCTGCTCGGAT
CACACCCCGGTGGACGACGACGAAAAACTCCTGCCTTTCGCCGAGGCCTCGCCGGGCGCTACAGGCCTGGAACTGCTGCT
GCCGCTGACGCTGCGCTGGGCCAGCGAGCACAAGGTGCCGCTCGTGCGCGCGCTGGCTCGCATTACCAGCGAACCCGCGC
GGGTTATCGGCCTGAATGTGGGATCGCTGACCACGGGCAGCATGGCCGACGTGTGCGTGTTCGACCCGAAGCAGCTCTGG
AAGGTGGAGCGCAGCGTGTTGAAGAGTCAGGGCAAGAACTCGCCGTGGCTCGGCTACGAGATGGAAGGGCGTGTGCGTAC
GACGCTGGTCTGCGGCCAGGTCGTCTACGAACAGCGCTGA

Upstream 100 bases:

>100_bases
CCGCCGTGGCAGACGGTCCGCAATCGGTGATCCTGAACCAGGTGACGTTCGGCATCGCCGTGCGCATGGCCGTGATGGGC
ATCGTGGCAGGTAATAACGA

Downstream 100 bases:

>100_bases
CAGGCCGGGCGCCATCGAGGCAACACGGGGTCGACGATGATCTGGTTACGCAAGGCGCGGCTGACATTTCACCTGCTGCG
TGGGGTGCTGTTCTGTGCCA

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKTIDARGMIVCPGLVDLSARLR
EPGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEA
GCVGFSQAEAPIHDTKVLQRAMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATG
ARVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGARDRDAIVAALADGTIDALCSD
HTPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVPLVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLW
KVERSVLKSQGKNSPWLGYEMEGRVRTTLVCGQVVYEQR

Sequences:

>Translated_439_residues
MSRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKTIDARGMIVCPGLVDLSARLR
EPGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEA
GCVGFSQAEAPIHDTKVLQRAMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATG
ARVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGARDRDAIVAALADGTIDALCSD
HTPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVPLVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLW
KVERSVLKSQGKNSPWLGYEMEGRVRTTLVCGQVVYEQR
>Mature_438_residues
SRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKTIDARGMIVCPGLVDLSARLRE
PGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEAG
CVGFSQAEAPIHDTKVLQRAMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATGA
RVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGARDRDAIVAALADGTIDALCSDH
TPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVPLVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLWK
VERSVLKSQGKNSPWLGYEMEGRVRTTLVCGQVVYEQR

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=390, Percent_Identity=26.6666666666667, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1786722, Length=427, Percent_Identity=25.9953161592506, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082175, Length=455, Percent_Identity=25.2747252747253, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=387, Percent_Identity=25.3229974160207, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322218, Length=422, Percent_Identity=24.8815165876777, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24642586, Length=398, Percent_Identity=24.6231155778894, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18859883, Length=210, Percent_Identity=29.0476190476191, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 47260; Mature: 47129

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKT
CCCCCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCEEEEECEEEECCCCCCCCCCCCE
IDARGMIVCPGLVDLSARLREPGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGL
ECCCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCH
VEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEAGCVGFSQAEAPIHDTKVLQR
HHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATG
HHHHHHHCCEEEEECCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
ARVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGAR
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECEEECCCEEECCCCCCCC
DRDAIVAALADGTIDALCSDHTPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVP
CCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHEEECCCCCCH
LVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLWKVERSVLKSQGKNSPWLGYE
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCEE
MEGRVRTTLVCGQVVYEQR
ECCCEEEEEEEHHHHHCCC
>Mature Secondary Structure 
SRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKT
CCCCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCEEEEECEEEECCCCCCCCCCCCE
IDARGMIVCPGLVDLSARLREPGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGL
ECCCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCH
VEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEAGCVGFSQAEAPIHDTKVLQR
HHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATG
HHHHHHHCCEEEEECCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
ARVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGAR
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECEEECCCEEECCCCCCCC
DRDAIVAALADGTIDALCSDHTPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVP
CCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHEEECCCCCCH
LVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLWKVERSVLKSQGKNSPWLGYE
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCEE
MEGRVRTTLVCGQVVYEQR
ECCCEEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]