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The map label for this gene is pyrB [H]

Identifier: 94311672

GI number: 94311672

Start: 2976207

End: 2977178

Strand: Reverse

Name: pyrB [H]

Synonym: Rmet_2740

Alternate gene names: 94311672

Gene position: 2977178-2976207 (Counterclockwise)

Preceding gene: 94311673

Following gene: 94311671

Centisome position: 75.79

GC content: 63.89

Gene sequence:

>972_bases
ATGACCAAGACGTTTCGCAACCCGCAGCTCACCAAGAACGGCGAACTGAAGCACCTGCTGTCGATCGAGGGGCTGTCGCG
TGACATGATCACGCACATTCTCGATACCGCCAGCCAGTTCGTATCGCTCTCCGACTCCGACCGCGAGGTCAAGAAGGTGC
CACTGCTGCGCGGCAAGAGCGTGTTCAATCTGTTCTTCGAGAACTCCACACGGACTCGCACTACGTTCGAGATCGCGGCC
AAGCGGCTGTCGGCGGACGTGCTGAACCTGAATATCAATGCGTCGTCCACGAGCAAGGGCGAATCGCTGCTCGATACGAT
CAACAACCTGTCGGCGATGTCGGCCGACATGTTCGTGGTGCGCCACGCCAGTTCCGGCGCGCCTTACCTGATCGCCGAAC
ACGTGGCCCCGCACGTGCACGTGATCAACGCCGGCGATGGCCGCCACGCGCACCCGACGCAGGGCCTGCTGGACATGTAC
ACGATCCGGCATTTCAAGAAGGACTTCACGAACCTGACGGTGGCGATCGTCGGCGACATCCTGCACTCGCGCGTGGCGCG
CTCGGACATCCACGCGCTGACCACGCTGGGCGTGCCGGAAGTCCGCGCGATCGGGCCGCGCACGCTGCTGCCGTCCGGGC
TGGAGCAGATGGGTGTGCGGGTCTTCCACAACATGGAAGAGGGCCTGAAGGGTGTGGACGTGGTGATCATGCTGCGCCTG
CAGAACGAGCGGATGACCGGCGCGCTGCTGCCGTCGGCGCAGGAGTACTTCAAGGCCTACGGGCTGACCCCGGAGCGCCT
GGCGCTGGCCAAGCCTGATGCCATCGTGATGCACCCGGGCCCGATGAACCGCGGCGTGGAGATCGACTCCGCCGTGGCAG
ACGGTCCGCAATCGGTGATCCTGAACCAGGTGACGTTCGGCATCGCCGTGCGCATGGCCGTGATGGGCATCGTGGCAGGT
AATAACGATTGA

Upstream 100 bases:

>100_bases
GCGAGGGTGCGGACACCCGCTTTGCCTTTGCCACTGAAGTCCGTAGCTGAACCCGCAAGCACGCAAGTACCACACAGATA
GAACCCGCGCCCGACCTGCC

Downstream 100 bases:

>100_bases
GCCGGACCCCGGATACAACGAACAGCACACACCGACAGAACATGAAGATCCATATCCAGGGTGGCCGCCTGATCGACCCG
GCCGCGAACGTCGACGCCGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMY
TIRHFKKDFTNLTVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRL
QNERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAG
NND

Sequences:

>Translated_323_residues
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMY
TIRHFKKDFTNLTVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRL
QNERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAG
NND
>Mature_322_residues
TKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAK
RLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYT
IRHFKKDFTNLTVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRLQ
NERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAGN
ND

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=35.3503184713376, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=35.2564102564103, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786469, Length=199, Percent_Identity=28.1407035175879, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=337, Percent_Identity=31.4540059347181, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=32.3809523809524, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24642586, Length=320, Percent_Identity=34.0625, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35248; Mature: 35117

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKS
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCHHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHFKKDFTNLTVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRL
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
QNERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCEECHHHCCCHHHHH
LNQVTFGIAVRMAVMGIVAGNND
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKS
CCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCHHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHFKKDFTNLTVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRL
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
QNERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCEECHHHCCCHHHHH
LNQVTFGIAVRMAVMGIVAGNND
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA