Click here to switch to the map view.

The map label for this gene is pspE2 [H]

Identifier: 94311642

GI number: 94311642

Start: 2942621

End: 2942944

Strand: Direct

Name: pspE2 [H]

Synonym: Rmet_2710

Alternate gene names: 94311642

Gene position: 2942621-2942944 (Clockwise)

Preceding gene: 94311641

Following gene: 94311643

Centisome position: 74.91

GC content: 62.65

Gene sequence:

>324_bases
ATGCAAGTCATTACCGCTCCCGAACTCGCCCAATGGCTGGCGGATGCCGGCCGCGCGAAACCGGTGCTGCTCGACGTACG
CGAGGACATCGAGATCCGGACCGCCGCAATGCCCGACATCACGCATATCCCGATGGGCCAGATTCCTGGCCGGCTGAATG
AACTCGACGAAGACGCCGAAATCGTCTGCATTTGTCACCACGGCGCGCGCAGCATGCAGGTGGCGAACTTCCTCGAGCGC
CAGGGTTATGCCAAGGTCTACAACCTGACCGGCGGCATTCACGCTTGGTCGACGCAGGTCGACCCGTCCGTCCCGCAGTA
CTGA

Upstream 100 bases:

>100_bases
GAGACCGAGTACCAGGTTGTCGACCTGTTCGAGACGCTGGCCAAGCCGCTGGAAGGCGCCGTCCAGCCGTCGCGCTTCAA
GTTCTGATCAGACGCCAGAT

Downstream 100 bases:

>100_bases
CCCGGGAACCGGCCCCGGGACGGCAACGCCGCCCGGGACCATCCCGCCCTGCAGCACCTCCGCAGTCCTTGCCGTACCAC
CCGTCGATGTAACCGAACAT

Product: Rhodanese-related sulfurtransferase

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MQVITAPELAQWLADAGRAKPVLLDVREDIEIRTAAMPDITHIPMGQIPGRLNELDEDAEIVCICHHGARSMQVANFLER
QGYAKVYNLTGGIHAWSTQVDPSVPQY

Sequences:

>Translated_107_residues
MQVITAPELAQWLADAGRAKPVLLDVREDIEIRTAAMPDITHIPMGQIPGRLNELDEDAEIVCICHHGARSMQVANFLER
QGYAKVYNLTGGIHAWSTQVDPSVPQY
>Mature_107_residues
MQVITAPELAQWLADAGRAKPVLLDVREDIEIRTAAMPDITHIPMGQIPGRLNELDEDAEIVCICHHGARSMQVANFLER
QGYAKVYNLTGGIHAWSTQVDPSVPQY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11865; Mature: 11865

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVITAPELAQWLADAGRAKPVLLDVREDIEIRTAAMPDITHIPMGQIPGRLNELDEDAE
CCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
IVCICHHGARSMQVANFLERQGYAKVYNLTGGIHAWSTQVDPSVPQY
EEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MQVITAPELAQWLADAGRAKPVLLDVREDIEIRTAAMPDITHIPMGQIPGRLNELDEDAE
CCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
IVCICHHGARSMQVANFLERQGYAKVYNLTGGIHAWSTQVDPSVPQY
EEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]