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The map label for this gene is pcm [H]

Identifier: 94311641

GI number: 94311641

Start: 2941954

End: 2942607

Strand: Direct

Name: pcm [H]

Synonym: Rmet_2709

Alternate gene names: 94311641

Gene position: 2941954-2942607 (Clockwise)

Preceding gene: 94311640

Following gene: 94311642

Centisome position: 74.9

GC content: 64.53

Gene sequence:

>654_bases
ATGGATCTCGAAAAAGCCCGCTTCAACATGATCGAACAGCAAATCCGCCCATGGGACGTGCTGGACCAGGAAATCCTGGA
CCTGCTGGCCGTGGTCAAGCGTGAGCAGTTCGTACCGCAGGCCTACGCCGCATTGGCTTTCGTCGACATGGAGATTCCGC
TGCCGTGCGGCGAGAACATGCTGCCCCCGCGCGTCGAGGCCCGTCTTCTGCAGGACCTGAACGTGCGCAAGCATGAGAAC
GTGCTCGAAATTGGCGCGGGCTCCGGCTACATGGCCGCCCTGCTGGCCAACCGCGCCCGTCACGTGCTCACCGTGGACGT
TCATCCCGAACTGGTTGCCCTGGCTCGCCAGAACCTGGCCAACGCCGGCATCACCAACGTTGACGTGGCAGAAGGCAATG
CCGCCGAAGGCTGGGCGGTCGCCGCGCCGTACGACGTGATCTGCATCTCGGCGTCCGTGCCGGAAATTCCGAGCGCGATC
CTGTCCCAGGTGAAGGTCGGCGGCCGTATCGCTGCCATCGTCGGCTCGGCCCCGGTCATGGAAGCCCGCATCATCACGCG
CGCCTCCGAGACCGAGTACCAGGTTGTCGACCTGTTCGAGACGCTGGCCAAGCCGCTGGAAGGCGCCGTCCAGCCGTCGC
GCTTCAAGTTCTGA

Upstream 100 bases:

>100_bases
CGGGACGTCACTCGGCATCAACCGCAACGACAGCGCTGACGGCGGCATCACCCGCTAACCGGCAAGCGCAACCCCGCAAG
CATTTGGAAGGAAAGGCAAG

Downstream 100 bases:

>100_bases
TCAGACGCCAGATATGCAAGTCATTACCGCTCCCGAACTCGCCCAATGGCTGGCGGATGCCGGCCGCGCGAAACCGGTGC
TGCTCGACGTACGCGAGGAC

Product: Protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENMLPPRVEARLLQDLNVRKHEN
VLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLANAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAI
LSQVKVGGRIAAIVGSAPVMEARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF

Sequences:

>Translated_217_residues
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENMLPPRVEARLLQDLNVRKHEN
VLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLANAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAI
LSQVKVGGRIAAIVGSAPVMEARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF
>Mature_217_residues
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENMLPPRVEARLLQDLNVRKHEN
VLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLANAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAI
LSQVKVGGRIAAIVGSAPVMEARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=184, Percent_Identity=34.2391304347826, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23663; Mature: 23663

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENM
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEECCCCCCCC
LPPRVEARLLQDLNVRKHENVLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLA
CCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHH
NAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAILSQVKVGGRIAAIVGSAPVM
HCCCCEEEECCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHH
EARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENM
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEECCCCCCCC
LPPRVEARLLQDLNVRKHENVLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLA
CCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHH
NAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAILSQVKVGGRIAAIVGSAPVM
HCCCCEEEECCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHH
EARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA