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The map label for this gene is tolC [H]

Identifier: 94311643

GI number: 94311643

Start: 2943081

End: 2944562

Strand: Direct

Name: tolC [H]

Synonym: Rmet_2711

Alternate gene names: 94311643

Gene position: 2943081-2944562 (Clockwise)

Preceding gene: 94311642

Following gene: 94311649

Centisome position: 74.92

GC content: 65.32

Gene sequence:

>1482_bases
ATGAGTTTTCCGCTTTCCGCCGTGCCCGGTGCGGCCAGGAGATCCCTGCCGCTCGCGGCTGCTCTTGTGGCCATGCTGCA
CTTGCCGGCCGCGGAGTCAGCCGATCTGCTGCAGGTGTATCGCGATGCGCAATCCAATGACGCCCAGTTCGCCAGCGCAC
GCGCGCAGCTCGTCGCCACGCGCGAGAAGCTGCCCCAGGCGCGCTCGGGCCTGTTGCCGCAGGTCTTTGGCACGGCAGGC
GCGCAGCGGACCCAGGCCGACTACAGCTCGCCGGTCGACGTCACCCGCTATTTCAATGCCAACACCTGGCAGTTGCAGCT
TACACAGCCGCTGTTCCGCTGGGATCGCTGGGAAACCTACAAGCAGGGAGAACTGGCCGCACTGGCGGGCGAGATCACCT
TCCAGCAGGCACAGCTCGACCTGATCACACGTGCCTCGCAGGCGTACTTCGATGTGTTGGCCGCGCAGGACAACCTCTAC
CTGGCGGGTGCCCAGAAGAAGGCCATTGGCGAGCAACTCGAGCAGGCCAAGCGCAATTTCGAGGTAGGCACCGCCACCAT
TACCGACGCCAACGATGCCCAGGCGCGCTACGACCTGGCCACCTCCACGGAGATTGCTTCGCAAAGCGCGCTCGAGGTGT
CCCGCGCCACGCTGCAACAGATCACCGGCAAATCCGTCGACGAACTGATGGGACTGCGCTCCGAAGCGGCGATTCCGGGC
CCGGCGCCGGCCGATGTCAACGCATGGGCGGCGCAGGCCGAACAGGCCAATCCGCAAGTCAGCCTGGCCGGATACAACCT
CGAAACGGCGCAGCGCGAAACGAACAAGGCCAAGGCAGGCCACCTGCCTTCCGTCGACCTCGTCGCTTCATACGGTTACA
CCAACGCCCAGGGCACGGCCACGCAGCTCAGTAACGTAGGCAGCCGCTACAACTCGGGCGTGGTGGGCGTCCAGTTGAGC
ATCCCGATCTTCACCGGTGGCCAGATCCAGTCGCGCGTGCGCGAAACCCTGGCGCTTGCCGACAAGGCCGCAAGCGATCT
CGAGTTCGCGCGCCGTACCGCCGCGCAGACCGCCCGGCAGACCTACTCGGGTGTGTACAACGGGCTCGCGCAGGTCAAGG
CGCTCGAGGCAGCGGAACGCTCGGCGCAAAGCGCAGTGGAATCGAACCAGCTTGGCTATGAAGTGGGCGTGCGCATCAAT
ATCGACGTGCTGAACGCGGAGGCGCAACTGTTCCAGACCCGCCGCGACCTGGCCAAGGCGCGCTACGACACGATCATGAA
TGGCATGCGCCTGAAGGCTTCCACCGGCGCGCTGTCGGAAGACGACGTGGTGCAGATCAACACGCTGCTGACGTCGGCGC
CGGGAGCGATGTACAAGTTGCCGGGGAAAACAAGAGTGGGTGCCATGCCCGCGCACAAGCCGGCACGCGGTGAACGCGGC
CGACGTGAAAGCATGGCTCAGGGTGTTACGCCACGGTCCTGA

Upstream 100 bases:

>100_bases
GACCATCCCGCCCTGCAGCACCTCCGCAGTCCTTGCCGTACCACCCGTCGATGTAACCGAACATTGGCCGCGCCGCGTCG
CAGGCCGCGCTGGAGATGTC

Downstream 100 bases:

>100_bases
CCGCCGGCAAGGTCAGACGGCGTCGGTCCGCGCCGAATGCGCCTCGCGCCCGGCCATCCCCAACAGCAGGCCGATGAGCC
CCGCGTAGAAAACGACGTTG

Product: type I secretion outer membrane efflux protein (TolC-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVATREKLPQARSGLLPQVFGTAG
AQRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETYKQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLY
LAGAQKKAIGEQLEQAKRNFEVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPG
PAPADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTATQLSNVGSRYNSGVVGVQLS
IPIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQTYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRIN
IDVLNAEAQLFQTRRDLAKARYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERG
RRESMAQGVTPRS

Sequences:

>Translated_493_residues
MSFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVATREKLPQARSGLLPQVFGTAG
AQRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETYKQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLY
LAGAQKKAIGEQLEQAKRNFEVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPG
PAPADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTATQLSNVGSRYNSGVVGVQLS
IPIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQTYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRIN
IDVLNAEAQLFQTRRDLAKARYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERG
RRESMAQGVTPRS
>Mature_492_residues
SFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVATREKLPQARSGLLPQVFGTAGA
QRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETYKQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLYL
AGAQKKAIGEQLEQAKRNFEVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPGP
APADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTATQLSNVGSRYNSGVVGVQLSI
PIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQTYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINI
DVLNAEAQLFQTRRDLAKARYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERGR
RESMAQGVTPRS

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=450, Percent_Identity=32.8888888888889, Blast_Score=215, Evalue=6e-57,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52911; Mature: 52780

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVAT
CCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
REKLPQARSGLLPQVFGTAGAQRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETY
HHHCCHHHHCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHCCC
KQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLYLAGAQKKAIGEQLEQAKRNF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCC
EVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPG
CCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCC
PAPADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTA
CCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHEECCCCCCCCHH
TQLSNVGSRYNSGVVGVQLSIPIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQ
HHHHHHHHHHCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINIDVLNAEAQLFQTRRDLAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHH
RYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERG
HHHHHHCCCEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
RRESMAQGVTPRS
HHHHHHCCCCCCC
>Mature Secondary Structure 
SFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVAT
CCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
REKLPQARSGLLPQVFGTAGAQRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETY
HHHCCHHHHCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHCCC
KQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLYLAGAQKKAIGEQLEQAKRNF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCC
EVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPG
CCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCC
PAPADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTA
CCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHEECCCCCCCCHH
TQLSNVGSRYNSGVVGVQLSIPIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQ
HHHHHHHHHHCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINIDVLNAEAQLFQTRRDLAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHH
RYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERG
HHHHHHCCCEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
RRESMAQGVTPRS
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]