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The map label for this gene is acsA [H]

Identifier: 94311631

GI number: 94311631

Start: 2931391

End: 2933400

Strand: Direct

Name: acsA [H]

Synonym: Rmet_2699

Alternate gene names: 94311631

Gene position: 2931391-2933400 (Clockwise)

Preceding gene: 94311630

Following gene: 94311633

Centisome position: 74.63

GC content: 65.07

Gene sequence:

>2010_bases
ATGACGCTTGCAACCCAGGCCGGACAGCCGGCATGGACGCCGTCCGCGGCATTCACCGAAAGCAGCCATCTGGCCGCCTA
TATGAAGTGGTTGCGCGGCGCGCGCGGCCTCGACTTCCGTGACTACGCCAGCCTCTGGCAATGGTCGGTCACCGACATCG
AAGCGTTCTGGCAGACCATCGTCGATTACTTCGACGTACGCTTCGACACACCCGCGCAGCGTGTACTGGATCGCCGCACC
ATGCCGGGCGCGCGCTGGTTCGAGGGCGCGACGCTCAACTACGCGCAGCAGATCTTCCGCCACGCCGGCACCGGCCTGGC
ACGCCAGCGCACCGCCATTCGCCATGCCGGCGAGGCGCAACCGCTAGCCGATATCAGTTGGGACACGCTGGAATCACAAG
TCGCCTCGGTTGCCCACGCCTTGCGCCAGATGGGCGTCACGCGCGGCGACCGCGTGGCTGGCTACCTGCCCAACGTGCCG
GAAACGATCGTCGCATTCCTGGCAACTGTCAGCGTGGGCGCGATCTGGTCGGGCTGCGCGCCGGACATGGGCCAGGTGGC
TGTGATCGACCGCTTCCGCCAGATCGAACCCAAGGTGTTGATCGCCGTCGATGGCTACCGATACGGCGGCAAGACGTACG
ATCGAGCGCCTGTGGTCGCCGATCTGGTTGCCGCGCTGCCCTCGCTGACCGACCTCGTGATCGTGCCGTCGATCGGCAGC
AACGTCACGCCCGAGCCACATGCCCGGCGCCACACCTGGAGCGACGTGCTGGCGCACGACGTGCCGCTCGAGATCACGCC
GGTCCCATTCGATCATCCGTTGTGGATCGTCTATTCGTCCGGCACCACCGGTATGCCAAAGCCAATCGTCCACGGGCACG
GCGGCATTGTGATCGAGCAGCTCAAGCTGATGGCATTCCACAACAACCTGGGCACAGATGATGTGTTTCACTGGTACAGC
AGCAGCGGCTGGATCATGTGGAATGCGCAGGTAGCTGGCCTGCTGCTGGGCACCACCATCGCGCTCTACGACGGCAACCC
GGCGTGGCCCGATGCCGGCGTGCTCTGGCGATTCGTGGATCAGGCGCGTGTGACGATGTTCGGCGCCGGCGCCGCATTCT
TCACCAATTGCATGAAGGCGGGCGTAGAACCGGCGGAGATCGCCGATCTGTCACGCCTGCGCGGTCTGGGCTCGACGGGA
TCGCCGCTGCCGGAGGAAGCGTACGACTGGATCTACGGGCATGTGCGCGCCGACATCTGGCTGGCGCCGATGTCCGGCGG
TACCGACTTCGCCGGATCGTTCGTGGCCGGATGCCCCCTGCTGCCGGTCTATCAGGGCGAAATGCAGTGCCGTTGCCTGG
GCGCCAAGGTCGAAGCCTTTGACGACAACGGCAAGCCACTGATCGACGAGGTGGGCGAACTGGTCTGCACCGAGCCGATG
CCATCCATGCCGCTGTTCCTGTGGGGCGATGCCGACGGCAAGCGTTACCGCGACAGCTACTTCGACACCTATCCGAACGC
GTGGCGCCACGGCGACTGGATTCGCATCACACCGCGCGGCGGCGCCATCATCTACGGTCGCTCCGATGCGACGATCAACC
GATACGGCATCCGCATGGGCACGAGCGAGCTGTATCGCGTGGTGGAAGAGTTGCCCGAAGTGCTCGACAGCATGGTGGTC
GATCTCGAATACCTCGGCCGCGAATCGTATATGCCACTGTTCGTGGTGCTGCGAGAGGGCATGGCGCTGGACGATGCCCT
GCGCGACACCCTGCGCGCGCGCATTCGCGTTGCGCTGTCGTCGCGGCACGTGCCAAACGAGATCCTGCAGGTGCCCGGCG
TGCCGCGCACGCTCTCGGGCAAAAAGATGGAAGTGCCGATCAAGAAGCTGCTGCTCGGTCATGCACCCGAGAAGATCGCC
AACCGCGATGCCATGGCGAATCCCGAGTGCCTGACGTGGTATTTCGCGTATGCGGAGGACTACCTGACGCGCGCGGCGCA
GCCGGCGTAA

Upstream 100 bases:

>100_bases
GCCCCGGCAAGTAGCGCGATTGCTCGCTATCATCGCTTTTTGACCGGCAATCCAAGACAACACTCTCATGGCCCGCGCAG
ACGGGCTCCGGAGGAGACAA

Downstream 100 bases:

>100_bases
GGCGCTACGTCAGAACTCGACGTCGAGTTCGCTGATCTCGTCGGGCTCCTCGCGGGCGGCATGGATCCATTCCTGCATCT
CGGGCATGGCCAGGATCGTC

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MTLATQAGQPAWTPSAAFTESSHLAAYMKWLRGARGLDFRDYASLWQWSVTDIEAFWQTIVDYFDVRFDTPAQRVLDRRT
MPGARWFEGATLNYAQQIFRHAGTGLARQRTAIRHAGEAQPLADISWDTLESQVASVAHALRQMGVTRGDRVAGYLPNVP
ETIVAFLATVSVGAIWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKTYDRAPVVADLVAALPSLTDLVIVPSIGS
NVTPEPHARRHTWSDVLAHDVPLEITPVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGTDDVFHWYS
SSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDQARVTMFGAGAAFFTNCMKAGVEPAEIADLSRLRGLGSTG
SPLPEEAYDWIYGHVRADIWLAPMSGGTDFAGSFVAGCPLLPVYQGEMQCRCLGAKVEAFDDNGKPLIDEVGELVCTEPM
PSMPLFLWGDADGKRYRDSYFDTYPNAWRHGDWIRITPRGGAIIYGRSDATINRYGIRMGTSELYRVVEELPEVLDSMVV
DLEYLGRESYMPLFVVLREGMALDDALRDTLRARIRVALSSRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPEKIA
NRDAMANPECLTWYFAYAEDYLTRAAQPA

Sequences:

>Translated_669_residues
MTLATQAGQPAWTPSAAFTESSHLAAYMKWLRGARGLDFRDYASLWQWSVTDIEAFWQTIVDYFDVRFDTPAQRVLDRRT
MPGARWFEGATLNYAQQIFRHAGTGLARQRTAIRHAGEAQPLADISWDTLESQVASVAHALRQMGVTRGDRVAGYLPNVP
ETIVAFLATVSVGAIWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKTYDRAPVVADLVAALPSLTDLVIVPSIGS
NVTPEPHARRHTWSDVLAHDVPLEITPVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGTDDVFHWYS
SSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDQARVTMFGAGAAFFTNCMKAGVEPAEIADLSRLRGLGSTG
SPLPEEAYDWIYGHVRADIWLAPMSGGTDFAGSFVAGCPLLPVYQGEMQCRCLGAKVEAFDDNGKPLIDEVGELVCTEPM
PSMPLFLWGDADGKRYRDSYFDTYPNAWRHGDWIRITPRGGAIIYGRSDATINRYGIRMGTSELYRVVEELPEVLDSMVV
DLEYLGRESYMPLFVVLREGMALDDALRDTLRARIRVALSSRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPEKIA
NRDAMANPECLTWYFAYAEDYLTRAAQPA
>Mature_668_residues
TLATQAGQPAWTPSAAFTESSHLAAYMKWLRGARGLDFRDYASLWQWSVTDIEAFWQTIVDYFDVRFDTPAQRVLDRRTM
PGARWFEGATLNYAQQIFRHAGTGLARQRTAIRHAGEAQPLADISWDTLESQVASVAHALRQMGVTRGDRVAGYLPNVPE
TIVAFLATVSVGAIWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKTYDRAPVVADLVAALPSLTDLVIVPSIGSN
VTPEPHARRHTWSDVLAHDVPLEITPVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGTDDVFHWYSS
SGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDQARVTMFGAGAAFFTNCMKAGVEPAEIADLSRLRGLGSTGS
PLPEEAYDWIYGHVRADIWLAPMSGGTDFAGSFVAGCPLLPVYQGEMQCRCLGAKVEAFDDNGKPLIDEVGELVCTEPMP
SMPLFLWGDADGKRYRDSYFDTYPNAWRHGDWIRITPRGGAIIYGRSDATINRYGIRMGTSELYRVVEELPEVLDSMVVD
LEYLGRESYMPLFVVLREGMALDDALRDTLRARIRVALSSRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPEKIAN
RDAMANPECLTWYFAYAEDYLTRAAQPA

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=659, Percent_Identity=38.0880121396055, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI28416953, Length=620, Percent_Identity=26.4516129032258, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI8923896, Length=683, Percent_Identity=24.3045387994143, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI253970489, Length=696, Percent_Identity=23.8505747126437, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI13375727, Length=641, Percent_Identity=24.1809672386895, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1790505, Length=629, Percent_Identity=25.9141494435612, Blast_Score=185, Evalue=9e-48,
Organism=Escherichia coli, GI1786529, Length=622, Percent_Identity=26.0450160771704, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17569507, Length=636, Percent_Identity=34.4339622641509, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71983001, Length=637, Percent_Identity=24.8037676609105, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982997, Length=637, Percent_Identity=24.9607535321821, Blast_Score=154, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323182, Length=653, Percent_Identity=26.4931087289433, Blast_Score=193, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6319264, Length=634, Percent_Identity=27.1293375394322, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24667955, Length=686, Percent_Identity=25.3644314868805, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI62472339, Length=556, Percent_Identity=26.6187050359712, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21355225, Length=603, Percent_Identity=24.8756218905473, Blast_Score=152, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24667959, Length=525, Percent_Identity=26.2857142857143, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 74037; Mature: 73906

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLATQAGQPAWTPSAAFTESSHLAAYMKWLRGARGLDFRDYASLWQWSVTDIEAFWQTI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
VDYFDVRFDTPAQRVLDRRTMPGARWFEGATLNYAQQIFRHAGTGLARQRTAIRHAGEAQ
HHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
PLADISWDTLESQVASVAHALRQMGVTRGDRVAGYLPNVPETIVAFLATVSVGAIWSGCA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKTYDRAPVVADLVAALPSLTDLVIVPSIGS
CCCCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
NVTPEPHARRHTWSDVLAHDVPLEITPVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQ
CCCCCCCCHHCCHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHH
LKLMAFHNNLGTDDVFHWYSSSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVD
HHHHHHHCCCCCCHHHHEECCCCEEEECHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHH
QARVTMFGAGAAFFTNCMKAGVEPAEIADLSRLRGLGSTGSPLPEEAYDWIYGHVRADIW
HHHEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEE
LAPMSGGTDFAGSFVAGCPLLPVYQGEMQCRCLGAKVEAFDDNGKPLIDEVGELVCTEPM
EEECCCCCCCCCHHHHCCCCCEEECCCCEEEEECCEEEEECCCCCCHHHHHHHEEECCCC
PSMPLFLWGDADGKRYRDSYFDTYPNAWRHGDWIRITPRGGAIIYGRSDATINRYGIRMG
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEHEEECC
TSELYRVVEELPEVLDSMVVDLEYLGRESYMPLFVVLREGMALDDALRDTLRARIRVALS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPEKIANRDAMANPECLTWYFAYAED
CCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHCCCCHHHHCCCCCCCCCCEEHHHHHHHH
YLTRAAQPA
HHHHCCCCC
>Mature Secondary Structure 
TLATQAGQPAWTPSAAFTESSHLAAYMKWLRGARGLDFRDYASLWQWSVTDIEAFWQTI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
VDYFDVRFDTPAQRVLDRRTMPGARWFEGATLNYAQQIFRHAGTGLARQRTAIRHAGEAQ
HHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
PLADISWDTLESQVASVAHALRQMGVTRGDRVAGYLPNVPETIVAFLATVSVGAIWSGCA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKTYDRAPVVADLVAALPSLTDLVIVPSIGS
CCCCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
NVTPEPHARRHTWSDVLAHDVPLEITPVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQ
CCCCCCCCHHCCHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHH
LKLMAFHNNLGTDDVFHWYSSSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVD
HHHHHHHCCCCCCHHHHEECCCCEEEECHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHH
QARVTMFGAGAAFFTNCMKAGVEPAEIADLSRLRGLGSTGSPLPEEAYDWIYGHVRADIW
HHHEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEE
LAPMSGGTDFAGSFVAGCPLLPVYQGEMQCRCLGAKVEAFDDNGKPLIDEVGELVCTEPM
EEECCCCCCCCCHHHHCCCCCEEECCCCEEEEECCEEEEECCCCCCHHHHHHHEEECCCC
PSMPLFLWGDADGKRYRDSYFDTYPNAWRHGDWIRITPRGGAIIYGRSDATINRYGIRMG
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCEEEEHEEECC
TSELYRVVEELPEVLDSMVVDLEYLGRESYMPLFVVLREGMALDDALRDTLRARIRVALS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SRHVPNEILQVPGVPRTLSGKKMEVPIKKLLLGHAPEKIANRDAMANPECLTWYFAYAED
CCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHCCCCHHHHCCCCCCCCCCEEHHHHHHHH
YLTRAAQPA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]