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The map label for this gene is rfaC [H]

Identifier: 94311162

GI number: 94311162

Start: 2437871

End: 2438893

Strand: Reverse

Name: rfaC [H]

Synonym: Rmet_2224

Alternate gene names: 94311162

Gene position: 2438893-2437871 (Counterclockwise)

Preceding gene: 94311163

Following gene: 94311155

Centisome position: 62.09

GC content: 66.28

Gene sequence:

>1023_bases
ATGAAGCGCATCCTACTGATCAAGATCACGTCGCTCGGTGACATGGTGCATACGTTACCGCTGGTCCACGATCTGCGACG
CGCCTATCCCGGCGCGAAAATCGACTGGGTAGCCGATGCCTCCTGCGCGGACATCCCGCGTTGGACGGTCGGCATTGATC
GCGTGATTGCGCCGCCGCTGCGCCAGTTCAAGAAAAACGGCAAGAAGTGGCGAGACCTGCGTGGAATTCTCGGCGCGGTG
CGCGATTTGCGCCGCGAGCCCTATGACGTGGCCATCGACGTGCATGGCGTCTACAAGAGCGCCATCGTTGCGCGCCTGTC
CCGGACCCGCCGCCGCCTCGGGTATGCCGCCGAATTTCTAGGTGAGTCCCGCGCCGAATTTGCCTATACCGAACTGTTCG
GGCCGCACGGCGATGCGGACTGCCGGCACAAGATGCGCCTCGTGGTGGCCAATGCGCTCGGCTACGAGATCGAGCCGCAG
GAAACCTGCGAGTTGAGCATTCCGAAGCCGGCCGCGCCGCTCGCCGCAGACGGCGTGCCGCGTGCGATGCTGTTTCACGC
TACGTCCGCCGAAATCAAGAAGTGGCCGCAGGCAAACTGGATCGAGGTGGGCCACGCGCTCTCGCTCCGGGGCTACCGCG
TGCAGTTGCCGTGGGGATCGCAGCAGGAGCACGAAGAGGCGAAGGCACTTGCGGCCGCCATTCCTGGCGCGGAAGTGCTG
CCGCGCATGTCGATCACGGAGTGCGCGCAGCGCGTCGATGCGGCGGCGCTGGTCGTCGGCATGGACACGGGGTTCGTGCA
CCTGGCAGATGCGCTCGGCAAGCCCACGGTCATGCTCTTCACCGCCACGTCCCGGCCGCATTTCGGCGTGAATCGGCCTG
GCCAGTCGATCTCGGTCGGCGACAACGGCGTGCGCCCTCCGGTCGACGAGGTGCTGGCGGCGGTTGCCCAGGTCACTGGG
CACGGTGCCGGCGCAAGTCAGGGATTGCCCCGCGATCGTGCTACGGGCGACGTGTCGGCGTAA

Upstream 100 bases:

>100_bases
GATGCTGTGCGCGACTGGCTGAGGCATACTAGCGCCTTCCAAAACGCGGCCTGTCCGGCGGCGCCCCATTTTGCCGGCCA
TTGTGGCCGACCCACACCTC

Downstream 100 bases:

>100_bases
CGCCAGTAGTTGAATGCGACGATCGTGGCGCCGGCATGCGCCGTGACCAGATCCAGCATGAACGGCAGTTGGCGAATCCG
TAGTCCGATCATGTAACGGG

Product: Lipopolysaccharide heptosyltransferase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKRILLIKITSLGDMVHTLPLVHDLRRAYPGAKIDWVADASCADIPRWTVGIDRVIAPPLRQFKKNGKKWRDLRGILGAV
RDLRREPYDVAIDVHGVYKSAIVARLSRTRRRLGYAAEFLGESRAEFAYTELFGPHGDADCRHKMRLVVANALGYEIEPQ
ETCELSIPKPAAPLAADGVPRAMLFHATSAEIKKWPQANWIEVGHALSLRGYRVQLPWGSQQEHEEAKALAAAIPGAEVL
PRMSITECAQRVDAAALVVGMDTGFVHLADALGKPTVMLFTATSRPHFGVNRPGQSISVGDNGVRPPVDEVLAAVAQVTG
HGAGASQGLPRDRATGDVSA

Sequences:

>Translated_340_residues
MKRILLIKITSLGDMVHTLPLVHDLRRAYPGAKIDWVADASCADIPRWTVGIDRVIAPPLRQFKKNGKKWRDLRGILGAV
RDLRREPYDVAIDVHGVYKSAIVARLSRTRRRLGYAAEFLGESRAEFAYTELFGPHGDADCRHKMRLVVANALGYEIEPQ
ETCELSIPKPAAPLAADGVPRAMLFHATSAEIKKWPQANWIEVGHALSLRGYRVQLPWGSQQEHEEAKALAAAIPGAEVL
PRMSITECAQRVDAAALVVGMDTGFVHLADALGKPTVMLFTATSRPHFGVNRPGQSISVGDNGVRPPVDEVLAAVAQVTG
HGAGASQGLPRDRATGDVSA
>Mature_340_residues
MKRILLIKITSLGDMVHTLPLVHDLRRAYPGAKIDWVADASCADIPRWTVGIDRVIAPPLRQFKKNGKKWRDLRGILGAV
RDLRREPYDVAIDVHGVYKSAIVARLSRTRRRLGYAAEFLGESRAEFAYTELFGPHGDADCRHKMRLVVANALGYEIEPQ
ETCELSIPKPAAPLAADGVPRAMLFHATSAEIKKWPQANWIEVGHALSLRGYRVQLPWGSQQEHEEAKALAAAIPGAEVL
PRMSITECAQRVDAAALVVGMDTGFVHLADALGKPTVMLFTATSRPHFGVNRPGQSISVGDNGVRPPVDEVLAAVAQVTG
HGAGASQGLPRDRATGDVSA

Specific function: Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790051, Length=289, Percent_Identity=34.9480968858132, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011908 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 36943; Mature: 36943

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILLIKITSLGDMVHTLPLVHDLRRAYPGAKIDWVADASCADIPRWTVGIDRVIAPPL
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCHHHHHCHHH
RQFKKNGKKWRDLRGILGAVRDLRREPYDVAIDVHGVYKSAIVARLSRTRRRLGYAAEFL
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
GESRAEFAYTELFGPHGDADCRHKMRLVVANALGYEIEPQETCELSIPKPAAPLAADGVP
CCCHHHHHHHHHCCCCCCHHHHHHHHHEEHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
RAMLFHATSAEIKKWPQANWIEVGHALSLRGYRVQLPWGSQQEHEEAKALAAAIPGAEVL
CEEEEECCHHHHHHCCCCCCEECCCEEEECCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
PRMSITECAQRVDAAALVVGMDTGFVHLADALGKPTVMLFTATSRPHFGVNRPGQSISVG
CCCCHHHHHHHHCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEECC
DNGVRPPVDEVLAAVAQVTGHGAGASQGLPRDRATGDVSA
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRILLIKITSLGDMVHTLPLVHDLRRAYPGAKIDWVADASCADIPRWTVGIDRVIAPPL
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCHHHHHCHHH
RQFKKNGKKWRDLRGILGAVRDLRREPYDVAIDVHGVYKSAIVARLSRTRRRLGYAAEFL
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
GESRAEFAYTELFGPHGDADCRHKMRLVVANALGYEIEPQETCELSIPKPAAPLAADGVP
CCCHHHHHHHHHCCCCCCHHHHHHHHHEEHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
RAMLFHATSAEIKKWPQANWIEVGHALSLRGYRVQLPWGSQQEHEEAKALAAAIPGAEVL
CEEEEECCHHHHHHCCCCCCEECCCEEEECCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
PRMSITECAQRVDAAALVVGMDTGFVHLADALGKPTVMLFTATSRPHFGVNRPGQSISVG
CCCCHHHHHHHHCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEECC
DNGVRPPVDEVLAAVAQVTGHGAGASQGLPRDRATGDVSA
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8478319; 8041620; 9278503; 1624462 [H]