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The map label for this gene is hypE1 [H]

Identifier: 94310225

GI number: 94310225

Start: 1401177

End: 1402289

Strand: Reverse

Name: hypE1 [H]

Synonym: Rmet_1281

Alternate gene names: 94310225

Gene position: 1402289-1401177 (Counterclockwise)

Preceding gene: 94310226

Following gene: 94310221

Centisome position: 35.7

GC content: 72.15

Gene sequence:

>1113_bases
ATGAGAACCGAACACCACCAGGCCGGCGCTGGCGAGGCCCCCGCCCCCAAGCCCGTGAAGAAAAACTACATCCGCCCGCT
CGACATCAAACACGGCCGCATCGACATGGGCCACGGCGCGGGCGGCAAGGCCGCGGCGCAGCTGATCGAGGAGCTGTTCC
TGGCCGCCTTCGACAACGAATTCCTGCGCCAGGGCGACGACGGCGCGGTGCTGGCGCTGCCGGCCTTCGACCCGGCCGAG
GGGCGGCTGGTGATGGCGACCGACGGCCATGTGATCTCGCCGCTGTTCTTCCCCGGTGGCGACATCGGCTGCCTGTCGGT
GCACGGCACGGTCAACGACGTGGCCATGTCGGGCGCGCGGCCGCTGTACCTGAGCGCCAGCTTCATCATCGAAGAGGGCT
TCCCGCTGGTCGACCTGCAGCGCATCGTGCAGAGCATGGCCGCGGCAGCGCGCGAGGCCGGCGTGCCAGTGGTCACCGGC
GACACCAAGGTGGTGGAGCAGGGCAAGGGCGACGGCGTGTTCATCAGCACCACCGGCGTCGGCGTGCTGCCGCCGGGCGT
GTCCATCAGCGGCCGCAACGCCCGGCCGGGCGACGTGGTGCTGGTCTCGGGCAGCATCGGCGACCACGGCGTGGCCGTGC
TCTCGCAGCGCGAGTCGCTGGCCTTCGAGACCACGATCGAAAGCGACACCGCTGCGCTGCACACCCTGGTGGCCGCCATG
CTCGCGGCCGCGCCGGGCGCGGTGCGCGTGCTGCGCGACCCGACGCGCGGTGGCCTGGCGACCACGCTCAACGAGGTCGC
GCGCCAGTCGGGCGTGGGCATGCTGCTGCAGGAGGCCGCCATCCCGGTCAAGCCGCAGGTGGACGCCGCCTGCGAGCTGC
TGGGCCTGGACCCGCTCTACGTGGCCAACGAGGGCAAGCTGATCGCAGTGGTCGCGCCCGAGGCGGCCGAGGCCGTGCTG
GCCGCCATGCGCGCCCACCCGCTGGGGCAGGACGCGGCCCGCATCGGCGAGGTCACCACCGACCCGCACCACTTCGTGCA
GATGGCCACGCGCTTCGGCGGGCGGCGGGTGGTGGACTGGCTGAGTGGGGAGCAGCTGCCGAGGATTTGTTGA

Upstream 100 bases:

>100_bases
GCATGGTGTCCAGCGAAGGCGCCTGCGCCGCGCACTACACCTATGGCCGCTTCCGCGACATCCCCATCGTCGCCGCCACC
GCCACCGAGGAGGTCACCCC

Downstream 100 bases:

>100_bases
CACCCCCCTGCGCCGCTGCGCGGCTTCCCCCCGCTCTGGCGCGCCTTCGGCGCTGGGCAGGGGGACGGCACCTGAGGCCC
GGCGGAGCCGGTTCCTCGGT

Product: carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNEFLRQGDDGAVLALPAFDPAE
GRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGARPLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTG
DTKVVEQGKGDGVFISTTGVGVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAM
LAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLYVANEGKLIAVVAPEAAEAVL
AAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDWLSGEQLPRIC

Sequences:

>Translated_370_residues
MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNEFLRQGDDGAVLALPAFDPAE
GRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGARPLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTG
DTKVVEQGKGDGVFISTTGVGVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAM
LAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLYVANEGKLIAVVAPEAAEAVL
AAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDWLSGEQLPRIC
>Mature_370_residues
MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNEFLRQGDDGAVLALPAFDPAE
GRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGARPLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTG
DTKVVEQGKGDGVFISTTGVGVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAM
LAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLYVANEGKLIAVVAPEAAEAVL
AAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDWLSGEQLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=338, Percent_Identity=49.4082840236686, Blast_Score=303, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 38460; Mature: 38460

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNE
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCH
FLRQGDDGAVLALPAFDPAEGRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGAR
HHHCCCCCCEEEEECCCCCCCEEEEEECCCEECEEECCCCCEEEEEECCCHHHHHHCCCC
PLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTGDTKVVEQGKGDGVFISTTGV
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCC
GVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAM
EEECCCCEECCCCCCCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHH
LAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLY
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEE
VANEGKLIAVVAPEAAEAVLAAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDW
EECCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEHHH
LSGEQLPRIC
HCCCCCCCCC
>Mature Secondary Structure
MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNE
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCH
FLRQGDDGAVLALPAFDPAEGRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGAR
HHHCCCCCCEEEEECCCCCCCEEEEEECCCEECEEECCCCCEEEEEECCCHHHHHHCCCC
PLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTGDTKVVEQGKGDGVFISTTGV
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCC
GVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAM
EEECCCCEECCCCCCCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHH
LAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLY
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEE
VANEGKLIAVVAPEAAEAVLAAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDW
EECCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEHHH
LSGEQLPRIC
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8352644; 12948488; 8021224 [H]