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The map label for this gene is amtB [H]

Identifier: 94309186

GI number: 94309186

Start: 256874

End: 258406

Strand: Direct

Name: amtB [H]

Synonym: Rmet_0241

Alternate gene names: 94309186

Gene position: 256874-258406 (Clockwise)

Preceding gene: 94309185

Following gene: 94309187

Centisome position: 6.54

GC content: 67.38

Gene sequence:

>1533_bases
ATGAAAACCTGGTTCAAGCGAATCCTGACGGCCGGCGCGATGACGCTGGCGCTCGGCACGGCCGGTCTCGGCCTCTCCAC
CCACGCCGTGGCGCAGGACAAGCCCGCCGCGGAAGCATCGGCCGCGACGGCGTCCGCAACCCCCGCAGCCGCCGTTGCAG
CACCGGCTGCGGCCACGCCTGCCGCGGCGCCCGCCGAAGCTGCCGCCGCGCCGGCTGCCCCGGTGCCCAACAAGGGCGAC
ACGGCCTGGCTGCTGGTCTCCACCACATTCGTGATCCTGATGACGCTGCCGGGCCTGGCGCTGTTCTACGGTGGCCTGGT
CCGCTCGAAGAACATGCTGTCGGTGCTGATGCAGTGCCTGATGATCTTCTCGGTGATCTCGATCCTGTGGGCCATCTACG
GCTATAGCTTCGCGTTCACGGAGGGCAACGCCTTCTTCGGTGGCACGGACCGTCTGTTCATGAAGGGCCTGACGGTCGAT
GCCGTGGCCGCCACGTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCGTTCCAGTGCGCGTTCGCGGCGAT
CACTTGCAGCCTGATCATCGGCGCCTTCGCCGAGCGCGCCAAGTTCTCAGCGGTGCTGGTCTTCGTGGTCCTCTGGTTCA
CGTTCGCCTACATCCCGATGGCCCACATGGTCTGGTTCTGGCCCGGCCCGGATGCGTACACCGACGCCGCCGCAGGCACC
GCCGCCACCGCCAAGGCCGGCTGGCTGTTCCAGAAGGGCGCGCTCGATTTCGCGGGCGGCACGGTGGTGCACATCAACGC
GGCCGTGGCCGGTCTGGTGGGCGCGTTCATGTTCGGCAAGCGCATCGGCTTCGGTCGTGAAGCGATCCGCCCGCACAGCC
TGACCTTCACGATGGTGGGCGCCTCGCTGCTGTGGTTCGGCTGGTTCGGCTTCAACGCCGGTTCGGCCCTGGAAGCCAAC
GGCAGCGCCGCGCTGGCCTTCGTCAACACGCTGTTGGCCACGGCCGCCGCGGTGGTTTCCTGGACCTTCGGCGAGTGGAT
CGGCAAGGGCAAGCCGTCGATGCTCGGTGGCGCCTCGGGGGCTGTCGCCGGCCTGGTGGCGATTACCCCGGCCGCCGGCT
TCGTTGGCCCGATGGGCTCGATCGCGATGGGCCTGATCGCCGGCCTGCTGTGCCTGTGGGGCGTGACCGGTCTGAAGCGC
CTGCTGGGCATGGACGATTCGCTGGACGTGTTCGGTGTGCACGGCGTGGGCGGTATTCTCGGCGCGCTGCTGACCGGTGT
GTTCGCCGCGCCGAGCCTGGGCGGTACGGGCATCTATGACTACGTTGCCAACAAGGTGGCCGACGACTACTCGATCGCCG
GTCAGGTCTGGATCCAGTTCGAAGGTGTGCTGACCACGATCGTCTGGTCCGGCGTGGTCGCGCTGGTGGCCTACAAGCTG
ATCGATATGCTGATTGGCCTGCGCGTGCCGGAAGAGGAAGAGCGCGAAGGTCTGGATATCACCTCGCACGGTGAAACCGC
GTATGAGGTCTGA

Upstream 100 bases:

>100_bases
ATCTTCGTCGCACCCATAGAACAAGTCATTCGCATCCGCACCGGCGAGACCGGCGGCGATGCACTGTGACACGAGACACC
CCCGGAAAGAGGTTAAGCAA

Downstream 100 bases:

>100_bases
TATCTGCCAAGGTGTTGTGCTGGAGAGCTGATTGGGAATCTTCTCTCAGGAGTTTCGCCCCGGCCATGTGCCGGGGCTTT
TTTATTCTCGGGCTCGCCCC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKTWFKRILTAGAMTLALGTAGLGLSTHAVAQDKPAAEASAATASATPAAAVAAPAAATPAAAPAEAAAAPAAPVPNKGD
TAWLLVSTTFVILMTLPGLALFYGGLVRSKNMLSVLMQCLMIFSVISILWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVD
AVAATFSKGVVVPELGYFAFQCAFAAITCSLIIGAFAERAKFSAVLVFVVLWFTFAYIPMAHMVWFWPGPDAYTDAAAGT
AATAKAGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSALEAN
GSAALAFVNTLLATAAAVVSWTFGEWIGKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAMGLIAGLLCLWGVTGLKR
LLGMDDSLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQVWIQFEGVLTTIVWSGVVALVAYKL
IDMLIGLRVPEEEEREGLDITSHGETAYEV

Sequences:

>Translated_510_residues
MKTWFKRILTAGAMTLALGTAGLGLSTHAVAQDKPAAEASAATASATPAAAVAAPAAATPAAAPAEAAAAPAAPVPNKGD
TAWLLVSTTFVILMTLPGLALFYGGLVRSKNMLSVLMQCLMIFSVISILWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVD
AVAATFSKGVVVPELGYFAFQCAFAAITCSLIIGAFAERAKFSAVLVFVVLWFTFAYIPMAHMVWFWPGPDAYTDAAAGT
AATAKAGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSALEAN
GSAALAFVNTLLATAAAVVSWTFGEWIGKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAMGLIAGLLCLWGVTGLKR
LLGMDDSLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQVWIQFEGVLTTIVWSGVVALVAYKL
IDMLIGLRVPEEEEREGLDITSHGETAYEV
>Mature_510_residues
MKTWFKRILTAGAMTLALGTAGLGLSTHAVAQDKPAAEASAATASATPAAAVAAPAAATPAAAPAEAAAAPAAPVPNKGD
TAWLLVSTTFVILMTLPGLALFYGGLVRSKNMLSVLMQCLMIFSVISILWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVD
AVAATFSKGVVVPELGYFAFQCAFAAITCSLIIGAFAERAKFSAVLVFVVLWFTFAYIPMAHMVWFWPGPDAYTDAAAGT
AATAKAGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSALEAN
GSAALAFVNTLLATAAAVVSWTFGEWIGKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAMGLIAGLLCLWGVTGLKR
LLGMDDSLDVFGVHGVGGILGALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQVWIQFEGVLTTIVWSGVVALVAYKL
IDMLIGLRVPEEEEREGLDITSHGETAYEV

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=452, Percent_Identity=53.5398230088496, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17550182, Length=460, Percent_Identity=30, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=451, Percent_Identity=29.490022172949, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=463, Percent_Identity=24.4060475161987, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=24.8337028824834, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=35.1769911504425, Blast_Score=213, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6325396, Length=425, Percent_Identity=32.7058823529412, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=425, Percent_Identity=31.0588235294118, Blast_Score=203, Evalue=5e-53,
Organism=Drosophila melanogaster, GI161078314, Length=445, Percent_Identity=25.8426966292135, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52558; Mature: 52558

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWFKRILTAGAMTLALGTAGLGLSTHAVAQDKPAAEASAATASATPAAAVAAPAAATP
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCCCC
AAAPAEAAAAPAAPVPNKGDTAWLLVSTTFVILMTLPGLALFYGGLVRSKNMLSVLMQCL
CCCCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MIFSVISILWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVDAVAATFSKGVVVPELGYFAF
HHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHH
QCAFAAITCSLIIGAFAERAKFSAVLVFVVLWFTFAYIPMAHMVWFWPGPDAYTDAAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
AATAKAGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVG
CHHHHHCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHH
ASLLWFGWFGFNAGSALEANGSAALAFVNTLLATAAAVVSWTFGEWIGKGKPSMLGGASG
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
AVAGLVAITPAAGFVGPMGSIAMGLIAGLLCLWGVTGLKRLLGMDDSLDVFGVHGVGGIL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHH
GALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQVWIQFEGVLTTIVWSGVVALVAYKL
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IDMLIGLRVPEEEEREGLDITSHGETAYEV
HHHHHCCCCCCHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure
MKTWFKRILTAGAMTLALGTAGLGLSTHAVAQDKPAAEASAATASATPAAAVAAPAAATP
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCCCC
AAAPAEAAAAPAAPVPNKGDTAWLLVSTTFVILMTLPGLALFYGGLVRSKNMLSVLMQCL
CCCCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MIFSVISILWAIYGYSFAFTEGNAFFGGTDRLFMKGLTVDAVAATFSKGVVVPELGYFAF
HHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHH
QCAFAAITCSLIIGAFAERAKFSAVLVFVVLWFTFAYIPMAHMVWFWPGPDAYTDAAAGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
AATAKAGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVG
CHHHHHCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHH
ASLLWFGWFGFNAGSALEANGSAALAFVNTLLATAAAVVSWTFGEWIGKGKPSMLGGASG
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
AVAGLVAITPAAGFVGPMGSIAMGLIAGLLCLWGVTGLKRLLGMDDSLDVFGVHGVGGIL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHH
GALLTGVFAAPSLGGTGIYDYVANKVADDYSIAGQVWIQFEGVLTTIVWSGVVALVAYKL
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IDMLIGLRVPEEEEREGLDITSHGETAYEV
HHHHHCCCCCCHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]