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The map label for this gene is glnK [H]

Identifier: 94309185

GI number: 94309185

Start: 256504

End: 256842

Strand: Direct

Name: glnK [H]

Synonym: Rmet_0240

Alternate gene names: 94309185

Gene position: 256504-256842 (Clockwise)

Preceding gene: 94309184

Following gene: 94309186

Centisome position: 6.53

GC content: 62.24

Gene sequence:

>339_bases
ATGAAACTCATCATTGCAGTCATCAAGCCGTTCAAGCTCGACGAGGTGCGCGAGGCGCTCTCCGATGTGGGCGTGTCCGG
GATCACCGTGACCGAGGTCAAGGGCTTCGGCCGGCAGAAAGGCCACACGGAGCTGTATCGCGGCGCCGAATACATCGTCG
ACTTCCTTCCCAAGGTGAAGATCGAGGTGGCGGTGCCCGACGAAGTGGTGGAGCGAGCCATCGAGGCCGTGGAGAAGTCG
GCCCGCACCGGCAAGATCGGCGACGGCAAGATCTTCGTCGCACCCATAGAACAAGTCATTCGCATCCGCACCGGCGAGAC
CGGCGGCGATGCACTGTGA

Upstream 100 bases:

>100_bases
AACGTGGGCAACAACACCGCGTGGGTCTCGCTTTCCAAGACTTTCTAATTGCGCGTCAGTCAGGGTGGGCCGAAGCTGGC
CCGTCCAGAGGAGATAACCG

Downstream 100 bases:

>100_bases
CACGAGACACCCCCGGAAAGAGGTTAAGCAAATGAAAACCTGGTTCAAGCGAATCCTGACGGCCGGCGCGATGACGCTGG
CGCTCGGCACGGCCGGTCTC

Product: Nitrogen regulatory protein PII-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVKIEVAVPDEVVERAIEAVEKS
ARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL

Sequences:

>Translated_112_residues
MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVKIEVAVPDEVVERAIEAVEKS
ARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL
>Mature_112_residues
MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVKIEVAVPDEVVERAIEAVEKS
ARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=71.6981132075472, Blast_Score=160, Evalue=1e-41,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=67.8571428571429, Blast_Score=156, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12177; Mature: 12177

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVK
CEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCE
IEVAVPDEVVERAIEAVEKSARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL
EEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVK
CEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCE
IEVAVPDEVVERAIEAVEKSARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL
EEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1702507; 2878685 [H]