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The map label for this gene is gshA

Identifier: 94309187

GI number: 94309187

Start: 258869

End: 260164

Strand: Direct

Name: gshA

Synonym: Rmet_0242

Alternate gene names: NA

Gene position: 258869-260164 (Clockwise)

Preceding gene: 94309186

Following gene: 94309188

Centisome position: 6.59

GC content: 64.04

Gene sequence:

>1296_bases
ATGGTCCCGCATCTCATCACCGCGCTGAACGGTCCGCTGCTCGAACTCGAGAAGAAGATCCTGGACGCCACACCGTCCAT
CGAGCGCTGGTTCAGGCTGGAATGGCAGGAGCATACTCCGCCGTTCTATTGTTCCGTGGACCTGCGCAACGCCGGCTTCA
AGCTGGCGCCGGTCGACACCAACCTGTTCCCGGGCGGTTTCAACAACCTCGCGCCCGAAATGCTGCCGCTGGCGGTCCAG
GCCGCCATGGCCGCGATCGAGAAGATCTGCCCGGACGCCAAGAACCTGCTGCTGATTCCCGAGCGCCACACCCGCAACAT
GTTCTATCTGCAGAACGTGGCGCGGCTGTCGCTGATCATGCGCCAGGCGGGCCTCAATGTGCGCCTGGGCTCGCTGTCCG
AGGAAATCACCGAACCCACGCCGATCGAGCTGCCCGACGGCCAGACGCTGGTGGTGGAGCCGCTCGCGCGCCTTGGCGCC
AAGGGCCGCCGGCTGGGCCTGAAGGATTTCGATCCTTGCTCGATCCTGCTGAATAACGACCTGTCGGCCGGGATTCCGCC
GATCCTGGAGAACATCAACGAGCAGTACCTGCTGCCGCCGTTGCATGCCGGCTGGTCCACCCGCCGCAAGAGCAGCCACT
TCGCCGCCTATGACGAGGTGGCCAAGAAGTTCGCCAAGCTGATCGACATCGACCCGTGGATGGTGAATCCGTATTTCGCG
CGGCAGACTGGCGTGAATTTCCATGAGCGCATCGGCGAGGAAGAGCTGGCTGACGCGGTGGAAGGCGTGCTCAAGAAGAT
CGCGAAAAAGTACCGCGAGTACGGCATCAAGGAAACGCCCTACGTGGTGGTCAAGGCCGACGCCGGCACCTACGGCATGG
GCATCATGACCGTGCGCGACCCGTCCGAGGTGAAGGGCCTGAACCGCAAGGAGCGCAACAAGATGAGTGTCGTGAAGGAG
GGCCTCGAGGTCTCCGACGTCATCATCCAGGAAGGTGTGCACACCTTCGAAAAGGTCAACGACGCGGTGGCGGAGCCGGT
GGTCTACATGATCGACCGCTACGTGGTGGGCGGCTTCTACCGCGTGCACACGGGCCGTGGCATCGACGAGAACCTGAACG
CGCCGGGCATGCACTTCGTGCCGCTGGCCTTCGCGCCGAACGGTATGCCGGACAGCCACGCCAAGCCGGGCGCGGCCGTG
CCGAACCGCTTCTACATGTATGGCGTGGTGGCGCGCCTGGCCCTGCTGGCCGCGTCGCTGGAACTCGAGAAGACCGACCC
GAATCCGATCCTCTGA

Upstream 100 bases:

>100_bases
ATGCTGGGCTCCGCATATGCTTATTCAGGGCGGGCCTGCAGCCTCGTGGCCGTTCCACTACAATCGGGGCGGACAATAAA
GCTGATTCATTAGGATGGAT

Downstream 100 bases:

>100_bases
TTCGTAACGCCCCTGCAATCCACCATGCGCATTCTGTTCATTACCGACCCGCTGGAGACCTTCAAGACCTACAAGGACTC
CACCTTCGCCATGATGACCG

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGA
KGRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFA
RQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAV
PNRFYMYGVVARLALLAASLELEKTDPNPIL

Sequences:

>Translated_431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGA
KGRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFA
RQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAV
PNRFYMYGVVARLALLAASLELEKTDPNPIL
>Mature_431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGA
KGRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFA
RQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAV
PNRFYMYGVVARLALLAASLELEKTDPNPIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47956; Mature: 47956

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCCCHHHHEEECHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGAKGRRLGLKDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
LSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFA
CCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
RQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRD
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAVPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEKTDPNPIL
EEECCCCCCCC
>Mature Secondary Structure
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCCCHHHHEEECHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGAKGRRLGLKDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
LSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFA
CCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
RQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRD
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFY
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAVPNRFYMYGVVARLALLAASL
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEKTDPNPIL
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA