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The map label for this gene is dctA

Identifier: 94309173

GI number: 94309173

Start: 241551

End: 242840

Strand: Direct

Name: dctA

Synonym: Rmet_0228

Alternate gene names: 94309173

Gene position: 241551-242840 (Clockwise)

Preceding gene: 94309165

Following gene: 94309174

Centisome position: 6.15

GC content: 62.79

Gene sequence:

>1290_bases
ATGAGGAAACCCTTCTACAAGATCCTGTACGTGCAGGTCCTGTTCGCGATATTCGTGGGCATCCTGCTGGGCCACTACTG
GCCCGACACCGGCGTGGCCATGAAGCCGCTCGGTGACGCCTTCATCAAGCTGATCAAGATGATCATTGGTCCGATCATCT
TCTGTACCGTGGTGTCCGGCATTGCCGGCATGCGCGACATGAAGAAGGTGGGCCGCGTGGGTGGCAAGGCGCTGCTCTAC
TTCGAGATCGTGTCAACGTTCGCGCTGCTGCTCGGCCTGATCGCCACGCACGCGCTCAAACCGGGCGTGGGCTTCAACAT
CGATCCGGCCACGCTGGACACGAAGTCGATCGCGCAGTACGTGTCGAAGGCACACGGTCAGAGCACGGTCGAGTTCTTCA
TGCACATCATCCCGGACACGATCTTCAGCGCGTTCGCCAACGGCGACATCCTGCAGATCCTGATGGTCTCGATGTTCTTC
GGTTCGGCGCTGGCCGTGGTGGGTGAGCGCGCGCGCATCATCCACGACATGGTGGACCAGGTGTCGAAGGTGTTCTTCCA
CATCGTGCACGTGATCACGAAGGTGGCCCCGATCGGTGCATTCGGCGCGATGGCGTTCACCATCGGCAAGTACGGCCTGG
GCTCGCTGATTCCGCTGCTCAAGCTGATCGGCACGTTCTACCTCACGGCGATCGTGTTCGTGCTGGTGGTGCTGGGTACC
GTGGCGCGCCTGACCGGTTTCTCGATCATCCGCTTTGTCGCGTATATCAAGGAAGAACTGCTGATCGTGCTCGGCACCAG
CTCCTCCGAGGCTGCGCTGCCGCACCTGATGGAGAAGATGGAGAAGCTCGGCTGCTCGAAGTCGGTGGTGGGCCTGGTTG
TGCCGACCGGTTACTCGTTCAACCTGGACGGCACCAACATCTACATGACGATGGCGGTGATCTTCATCGCGCAGGCCACC
AACATCGAGCTGACGCTGATGCAGCAGCTCACGATCCTGGCCGTGGCGATGGTGACCTCGAAGGGCGCCAGCGGCGTGAC
CGGCTCGGGCTTCATCACGCTGGCAGCCACGCTGGCCGTGGTGCCGACGATTCCCGTGGCCGGCATGGTGCTGATCCTCG
GTATCGACCGCTTCATGAGCGAATGCCGCGCGCTGACGAACATCGTTGGCAACGGCGTGGCCACGGTGGTGGTCTCGGCG
TGGGAGCACGAGCTCGACCGCGCCCGCCTGACCCGCGTGCTGCGGGGCGAAGATGAGGCGAGCAGCCTCGCCGACGCCAG
CGCCCGCTGA

Upstream 100 bases:

>100_bases
GCAAATAGTTACTTCCAGACGCGCCGCCGTTCAGGTCGAGGCGCGCAACGCGCCACGCCATCGAGCGCCGGGGCACCGGT
CAAACACCGAGGACGACATC

Downstream 100 bases:

>100_bases
CGTGACCCGGGCAGGGACCGGCGCCCCGCAGGAAGGGGACGCCGGCGCTGTCCCGGATGAACCTGCCGGCCATCCCCGCC
AGCGGGTATCATCGGCGCGA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRKPFYKILYVQVLFAIFVGILLGHYWPDTGVAMKPLGDAFIKLIKMIIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEIVSTFALLLGLIATHALKPGVGFNIDPATLDTKSIAQYVSKAHGQSTVEFFMHIIPDTIFSAFANGDILQILMVSMFF
GSALAVVGERARIIHDMVDQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLIPLLKLIGTFYLTAIVFVLVVLGT
VARLTGFSIIRFVAYIKEELLIVLGTSSSEAALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
NIELTLMQQLTILAVAMVTSKGASGVTGSGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEHELDRARLTRVLRGEDEASSLADASAR

Sequences:

>Translated_429_residues
MRKPFYKILYVQVLFAIFVGILLGHYWPDTGVAMKPLGDAFIKLIKMIIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEIVSTFALLLGLIATHALKPGVGFNIDPATLDTKSIAQYVSKAHGQSTVEFFMHIIPDTIFSAFANGDILQILMVSMFF
GSALAVVGERARIIHDMVDQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLIPLLKLIGTFYLTAIVFVLVVLGT
VARLTGFSIIRFVAYIKEELLIVLGTSSSEAALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
NIELTLMQQLTILAVAMVTSKGASGVTGSGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEHELDRARLTRVLRGEDEASSLADASAR
>Mature_429_residues
MRKPFYKILYVQVLFAIFVGILLGHYWPDTGVAMKPLGDAFIKLIKMIIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEIVSTFALLLGLIATHALKPGVGFNIDPATLDTKSIAQYVSKAHGQSTVEFFMHIIPDTIFSAFANGDILQILMVSMFF
GSALAVVGERARIIHDMVDQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLIPLLKLIGTFYLTAIVFVLVVLGT
VARLTGFSIIRFVAYIKEELLIVLGTSSSEAALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
NIELTLMQQLTILAVAMVTSKGASGVTGSGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEHELDRARLTRVLRGEDEASSLADASAR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI4827012, Length=432, Percent_Identity=26.8518518518519, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI169790839, Length=428, Percent_Identity=25.4672897196262, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21314632, Length=425, Percent_Identity=24.7058823529412, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40254478, Length=439, Percent_Identity=24.6013667425968, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI66773030, Length=404, Percent_Identity=24.7524752475248, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI194239697, Length=491, Percent_Identity=24.0325865580448, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI5032093, Length=382, Percent_Identity=26.1780104712042, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI223468564, Length=259, Percent_Identity=27.7992277992278, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI223468566, Length=259, Percent_Identity=27.7992277992278, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI262359914, Length=374, Percent_Identity=23.7967914438503, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI301601644, Length=192, Percent_Identity=29.6875, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=417, Percent_Identity=60.9112709832134, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=40.0485436893204, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI1788024, Length=403, Percent_Identity=22.0843672456576, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=24.4582043343653, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=26.972010178117, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=483, Percent_Identity=25.879917184265, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=449, Percent_Identity=24.4988864142539, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71983106, Length=449, Percent_Identity=24.4988864142539, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996953, Length=446, Percent_Identity=24.4394618834081, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=418, Percent_Identity=24.1626794258373, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=430, Percent_Identity=22.7906976744186, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24583025, Length=426, Percent_Identity=23.7089201877934, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137668, Length=426, Percent_Identity=23.7089201877934, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583023, Length=426, Percent_Identity=23.7089201877934, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360483, Length=474, Percent_Identity=23.4177215189873, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137666, Length=474, Percent_Identity=23.4177215189873, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281360481, Length=474, Percent_Identity=23.4177215189873, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA1_RALME (Q1LRW2)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582383.1
- ProteinModelPortal:   Q1LRW2
- SMR:   Q1LRW2
- STRING:   Q1LRW2
- GeneID:   4037014
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0228
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   HIVHVIT
- PhylomeDB:   Q1LRW2
- ProtClustDB:   PRK01663
- BioCyc:   RMET266264:RMET_0228-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46032; Mature: 46032

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11668ee8)-; HASH(0x1241c768)-; HASH(0x1241c9a8)-; HASH(0x13de1aac)-; HASH(0x132cae64)-; HASH(0x13ee5fb0)-; HASH(0x13ef40e4)-; HASH(0x12c1f0c0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPFYKILYVQVLFAIFVGILLGHYWPDTGVAMKPLGDAFIKLIKMIIGPIIFCTVVSG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMRDMKKVGRVGGKALLYFEIVSTFALLLGLIATHALKPGVGFNIDPATLDTKSIAQY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
VSKAHGQSTVEFFMHIIPDTIFSAFANGDILQILMVSMFFGSALAVVGERARIIHDMVDQ
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLIPLLKLIGTFYLTAIVFVLVVLGT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARLTGFSIIRFVAYIKEELLIVLGTSSSEAALPHLMEKMEKLGCSKSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVIFIAQATNIELTLMQQLTILAVAMVTSKGASGVTGSGFITLAATLAV
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VPTIPVAGMVLILGIDRFMSECRALTNIVGNGVATVVVSAWEHELDRARLTRVLRGEDEA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SSLADASAR
HHHHCCCCH
>Mature Secondary Structure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HHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA