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The map label for this gene is dctB [H]

Identifier: 94309174

GI number: 94309174

Start: 242959

End: 244965

Strand: Direct

Name: dctB [H]

Synonym: Rmet_0229

Alternate gene names: 94309174

Gene position: 242959-244965 (Clockwise)

Preceding gene: 94309173

Following gene: 94309175

Centisome position: 6.19

GC content: 67.96

Gene sequence:

>2007_bases
ATGCCCCATTCCGACGACTTCCTTGCCGTGCATGACCCTGCCGCGCCCGGCCCGGTGCACGCGCCGGAGTCGAGCACGCG
CTGGTGGGGGTTTGCCGCCGCGCTGATGGTGGGGCTCCTGGCGCTGTGCTCGCTGACCTGGCTGTTTTCGGTCCAGCGCG
GCATCGCCGCATTGCAGCAGTCCACGGCCATGCGTGCCGACCGTTATGCGGCCACGCTTGAATCGACGCTGGACCGCTAC
GAATTCCTTCCCGCGCTGGTATCGCTGCACCCTTCGGTGCGCGCGCTGCTGGCCAATCCTGAAGACCCGGCCCGTATTGC
CGTGGCCAACAACTACCTGGCCGAGGTCAATACCCGGGCCCGCGCGTCGGCCACCTACGTCATCGCGGCCAGCGGGCTGG
CACTGGCCGCCAGCAATCATGGCGAGCCGGGCAGCTTCGTCGGTACCGACTACCGTTTCCGCCCGTACTTCCAGATGGCA
TCGCGCGGTCAGATTGGCCGCTTCTACGCGATCGGCATCACCAGCGACGAGCCTGGCTACTACATCGCCCAGCCGATCGA
GTCGGGCGGCAAGGTCATCGGCGTGACGGTGGTGAAGCTGAACCTCGAATGGTTCCAGCGCGCGGGCTCCGGTAGCACCG
AGCCACTGATGGTGTCCGACGACCACGGCGTGATCTTCCTGTCGTCGGTGCCGGGCTGGCAGTACCGCACGCTTCGGCCC
CTGCCGCCCACGCTTCAGGCCGAACTGCACAACACGCGTCAGTATCATGACGTCGATGTCACGCCGCTGCCGATCCAGCC
GCTGGATTCCCCGGTCTCGCGCTGGCTGGCCAGCACCACGCTCAACGATGGCGAACGGCTGGTGAGAGTTGGCGCCGCGG
GCAGCACGGGGGCCACGGCACGAGATGGCGATGACGGTCAAGGCGCGCACTTCGGTCTGTCCAGCGATGGCGCGGACCGC
TACCTGGAAATCAATCGCGCCGTCGGGCCCGCCGGCTGGAACATGCAGGTGATGGCGCAACTGGACCCGGTGCTGGCCAG
TGCGCGCACCGCGACGATTGGCGCGGCGCTGGCCTATGCGTGCATCTGCCTGCTGCTGGTGAACCTGCGCCAGCGCCGCC
AGCGCGCGCGTGACATGCAATACAGCCGCCGGCTGCTGGAAGCGGCCTACGATCAACTGGAACGGCGCGTGGAGGCCCGT
ACGGCCGACCTGATGGCGATCAACGAGCAACTCGAAGCCGAAGTGACCGAGCGCGCGCGGACCGAAGGCGAACTGCGCGC
GACGCAGGATGAACTGGTGCAGGCCAGCAAGCTGGCGGCGCTGGGGCAGATGGCAGCCGGCATCACCCATGAACTGAACC
AGCCGCTGGCGGCCCTGCGCACGTTCTCGGACAACACGCGCGTGTTGCTGGAGCGCGGCCAGCTCGATGCAGCCACCGGC
AATCTCTCCGCCATCGCTGATCTGACCGAGCGGATGGGCAAGATCACGGGCCAGCTCAAGCTGTTCGCGGGCAAGGCGCG
TCAGCGCCGCAATGCCGTGCGCGTGCGCGCCGCCATCGACCATGTGCTGCAACTGATCGCGCCACGGCTGGGTCGGGTGA
TGCTACGGGTGCGGGGGCTCGATGGCGACGCTGCGGACCTGGCCGTATGGGCCGACGAACTCAAGCTCGAGCAGGTGCTG
CTGAATCTCGTCGGCAATGCGCTCGATGCCATTGCAGCGGCCGGCGCCGAGGATGGCCGCGTCGATATCGCCGTCAGCGC
GACAGACGAGACCGTGACGCTGGTCGTGCGCGACAATGGACCCGGCATCGCGCCGGACGCGCTGCCACGGCTGTTCGAGC
CGTTCTTCACGACCAAGGAGATCGGTCAGGGGCTCGGCCTGGGGCTGGCGATTTCGTCGTCGATCGTGCGCGAGTTCGGC
GGGCAACTGACGGCCGGCAATGTCGAGGGCGGCGGCGCGGAATTCGTCGTGACACTGCGACGCTCGCGCCAGACGGCCAC
GGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCCCCGCAGGAAGGGGACGCCGGCGCTGTCCCGGATGAACCTGCCGGCCATCCCCGCCAGCGGGTATCATCGGCGC
GAGAGCTTCCGCGCGCGCCG

Downstream 100 bases:

>100_bases
GGCGCATGAACGGCGTCAAGGATGACAGGTAAAGACATGATTGCGATGCAGGACGGATTGCGTGTGCTGTTCGTCGAGGA
CGAACCACTGGTGCGTCAGG

Product: signal transduction histidine kinase regulating C4-dicarboxylate transport system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MPHSDDFLAVHDPAAPGPVHAPESSTRWWGFAAALMVGLLALCSLTWLFSVQRGIAALQQSTAMRADRYAATLESTLDRY
EFLPALVSLHPSVRALLANPEDPARIAVANNYLAEVNTRARASATYVIAASGLALAASNHGEPGSFVGTDYRFRPYFQMA
SRGQIGRFYAIGITSDEPGYYIAQPIESGGKVIGVTVVKLNLEWFQRAGSGSTEPLMVSDDHGVIFLSSVPGWQYRTLRP
LPPTLQAELHNTRQYHDVDVTPLPIQPLDSPVSRWLASTTLNDGERLVRVGAAGSTGATARDGDDGQGAHFGLSSDGADR
YLEINRAVGPAGWNMQVMAQLDPVLASARTATIGAALAYACICLLLVNLRQRRQRARDMQYSRRLLEAAYDQLERRVEAR
TADLMAINEQLEAEVTERARTEGELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRVLLERGQLDAATG
NLSAIADLTERMGKITGQLKLFAGKARQRRNAVRVRAAIDHVLQLIAPRLGRVMLRVRGLDGDAADLAVWADELKLEQVL
LNLVGNALDAIAAAGAEDGRVDIAVSATDETVTLVVRDNGPGIAPDALPRLFEPFFTTKEIGQGLGLGLAISSSIVREFG
GQLTAGNVEGGGAEFVVTLRRSRQTATA

Sequences:

>Translated_668_residues
MPHSDDFLAVHDPAAPGPVHAPESSTRWWGFAAALMVGLLALCSLTWLFSVQRGIAALQQSTAMRADRYAATLESTLDRY
EFLPALVSLHPSVRALLANPEDPARIAVANNYLAEVNTRARASATYVIAASGLALAASNHGEPGSFVGTDYRFRPYFQMA
SRGQIGRFYAIGITSDEPGYYIAQPIESGGKVIGVTVVKLNLEWFQRAGSGSTEPLMVSDDHGVIFLSSVPGWQYRTLRP
LPPTLQAELHNTRQYHDVDVTPLPIQPLDSPVSRWLASTTLNDGERLVRVGAAGSTGATARDGDDGQGAHFGLSSDGADR
YLEINRAVGPAGWNMQVMAQLDPVLASARTATIGAALAYACICLLLVNLRQRRQRARDMQYSRRLLEAAYDQLERRVEAR
TADLMAINEQLEAEVTERARTEGELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRVLLERGQLDAATG
NLSAIADLTERMGKITGQLKLFAGKARQRRNAVRVRAAIDHVLQLIAPRLGRVMLRVRGLDGDAADLAVWADELKLEQVL
LNLVGNALDAIAAAGAEDGRVDIAVSATDETVTLVVRDNGPGIAPDALPRLFEPFFTTKEIGQGLGLGLAISSSIVREFG
GQLTAGNVEGGGAEFVVTLRRSRQTATA
>Mature_667_residues
PHSDDFLAVHDPAAPGPVHAPESSTRWWGFAAALMVGLLALCSLTWLFSVQRGIAALQQSTAMRADRYAATLESTLDRYE
FLPALVSLHPSVRALLANPEDPARIAVANNYLAEVNTRARASATYVIAASGLALAASNHGEPGSFVGTDYRFRPYFQMAS
RGQIGRFYAIGITSDEPGYYIAQPIESGGKVIGVTVVKLNLEWFQRAGSGSTEPLMVSDDHGVIFLSSVPGWQYRTLRPL
PPTLQAELHNTRQYHDVDVTPLPIQPLDSPVSRWLASTTLNDGERLVRVGAAGSTGATARDGDDGQGAHFGLSSDGADRY
LEINRAVGPAGWNMQVMAQLDPVLASARTATIGAALAYACICLLLVNLRQRRQRARDMQYSRRLLEAAYDQLERRVEART
ADLMAINEQLEAEVTERARTEGELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRVLLERGQLDAATGN
LSAIADLTERMGKITGQLKLFAGKARQRRNAVRVRAAIDHVLQLIAPRLGRVMLRVRGLDGDAADLAVWADELKLEQVLL
NLVGNALDAIAAAGAEDGRVDIAVSATDETVTLVVRDNGPGIAPDALPRLFEPFFTTKEIGQGLGLGLAISSSIVREFGG
QLTAGNVEGGGAEFVVTLRRSRQTATA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=32.3275862068966, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=30.9322033898305, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=285, Percent_Identity=27.0175438596491, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=25, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=283, Percent_Identity=28.6219081272085, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=27.8884462151394, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=28.6821705426357, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71868; Mature: 71737

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSDDFLAVHDPAAPGPVHAPESSTRWWGFAAALMVGLLALCSLTWLFSVQRGIAALQQ
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STAMRADRYAATLESTLDRYEFLPALVSLHPSVRALLANPEDPARIAVANNYLAEVNTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCEEEEECCHHHHCCCCC
RASATYVIAASGLALAASNHGEPGSFVGTDYRFRPYFQMASRGQIGRFYAIGITSDEPGY
CCCEEEEEEECCEEEEECCCCCCCCCCCCCCEECHHHHHHCCCCCCEEEEEEECCCCCCE
YIAQPIESGGKVIGVTVVKLNLEWFQRAGSGSTEPLMVSDDHGVIFLSSVPGWQYRTLRP
EEECCCCCCCEEEEEEEEEEEHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
LPPTLQAELHNTRQYHDVDVTPLPIQPLDSPVSRWLASTTLNDGERLVRVGAAGSTGATA
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RDGDDGQGAHFGLSSDGADRYLEINRAVGPAGWNMQVMAQLDPVLASARTATIGAALAYA
CCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
CICLLLVNLRQRRQRARDMQYSRRLLEAAYDQLERRVEARTADLMAINEQLEAEVTERAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEGELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRVLLERGQLDAATG
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCC
NLSAIADLTERMGKITGQLKLFAGKARQRRNAVRVRAAIDHVLQLIAPRLGRVMLRVRGL
CHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECC
DGDAADLAVWADELKLEQVLLNLVGNALDAIAAAGAEDGRVDIAVSATDETVTLVVRDNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCC
PGIAPDALPRLFEPFFTTKEIGQGLGLGLAISSSIVREFGGQLTAGNVEGGGAEFVVTLR
CCCCHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEE
RSRQTATA
CCCCCCCC
>Mature Secondary Structure 
PHSDDFLAVHDPAAPGPVHAPESSTRWWGFAAALMVGLLALCSLTWLFSVQRGIAALQQ
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STAMRADRYAATLESTLDRYEFLPALVSLHPSVRALLANPEDPARIAVANNYLAEVNTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCEEEEECCHHHHCCCCC
RASATYVIAASGLALAASNHGEPGSFVGTDYRFRPYFQMASRGQIGRFYAIGITSDEPGY
CCCEEEEEEECCEEEEECCCCCCCCCCCCCCEECHHHHHHCCCCCCEEEEEEECCCCCCE
YIAQPIESGGKVIGVTVVKLNLEWFQRAGSGSTEPLMVSDDHGVIFLSSVPGWQYRTLRP
EEECCCCCCCEEEEEEEEEEEHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
LPPTLQAELHNTRQYHDVDVTPLPIQPLDSPVSRWLASTTLNDGERLVRVGAAGSTGATA
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RDGDDGQGAHFGLSSDGADRYLEINRAVGPAGWNMQVMAQLDPVLASARTATIGAALAYA
CCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
CICLLLVNLRQRRQRARDMQYSRRLLEAAYDQLERRVEARTADLMAINEQLEAEVTERAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEGELRATQDELVQASKLAALGQMAAGITHELNQPLAALRTFSDNTRVLLERGQLDAATG
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCC
NLSAIADLTERMGKITGQLKLFAGKARQRRNAVRVRAAIDHVLQLIAPRLGRVMLRVRGL
CHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEECC
DGDAADLAVWADELKLEQVLLNLVGNALDAIAAAGAEDGRVDIAVSATDETVTLVVRDNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCC
PGIAPDALPRLFEPFFTTKEIGQGLGLGLAISSSIVREFGGQLTAGNVEGGGAEFVVTLR
CCCCHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEE
RSRQTATA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]