Click here to switch to the map view.

The map label for this gene is dksA [H]

Identifier: 94309071

GI number: 94309071

Start: 132183

End: 132872

Strand: Reverse

Name: dksA [H]

Synonym: Rmet_0126

Alternate gene names: 94309071

Gene position: 132872-132183 (Counterclockwise)

Preceding gene: 94309072

Following gene: 94309070

Centisome position: 3.38

GC content: 63.77

Gene sequence:

>690_bases
ATGACAGCCGCAACAAAGACGAAGGAAAGCCGCAGCAAGACTGGTAGCGCTGCGGTGGAGACGCGCAAGGCAGCGCCTGC
AAAGGCGACGCCAGCTCCTCGCGGCAGTACGGCGGCGCACGCCGATGCGGGCGAGGCCAAGGGAGGCACGCGAAGCAGCA
AGACTGCAGCCAGCCAGAGTGAAACAGCCGCCCCGCGCAAGCGGACGGCCACTGCACCCGAGCGGACGACGACGCCGCCG
GTCGCATCAACAAGAGAATCAGCAGGGACCATGAGCAGCAACAAACTTCTGACTGAAGCTGAAATCCTGAAGATGAGCGA
CAAGGATTACATGAACGAGGCGCAACTCGCCTTCTTCAAAAATCGCCTTGAGCAGCTTCGCGACGAGATCCTCAAGAATG
CGGACCAGACGACGGAACACCTGCGCGAAACGGTCATCGTGCCGGATCCGGCTGACCGCGCCACGATCGAGGAAGAACAC
GCCCTGGAACTGCGCACGCGCGACCGCGAGCGCAAGCTGCTGAAGAAGGTCGAGCAATCGCTGCAGCGCATCGAATCCGG
CGACTATGGCTGGTGCGAGGAAACGGGCGAGCCGATCGGCGTGCCGCGCCTGCTGGCCCGTCCGACCGCCACGCTGTCGC
TGGAAGCGCAACAGCGCCGCGAACTGCGCCAGAAGCTGTTCGGGGACTGA

Upstream 100 bases:

>100_bases
GCCAGCAGGCGCGGTCGTGGGCTTTGTGCGGAGACCGCCCTCCGCCTGGGGTCGCCTCGACAATGCGCCGTCACTCTTCG
ACGCGTAGAGAGGTGTCCCC

Downstream 100 bases:

>100_bases
TTCGATCCTTCGAGAGGCGGAAAACTCGGCGGCCCGGTGATCCGGGCCGCTTTTTCTTTTCCGGAGTCGTATTTGCCTTG
ATGGTGTAACGATTGCGACA

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTAATKTKESRSKTGSAAVETRKAAPAKATPAPRGSTAAHADAGEAKGGTRSSKTAASQSETAAPRKRTATAPERTTTPP
VASTRESAGTMSSNKLLTEAEILKMSDKDYMNEAQLAFFKNRLEQLRDEILKNADQTTEHLRETVIVPDPADRATIEEEH
ALELRTRDRERKLLKKVEQSLQRIESGDYGWCEETGEPIGVPRLLARPTATLSLEAQQRRELRQKLFGD

Sequences:

>Translated_229_residues
MTAATKTKESRSKTGSAAVETRKAAPAKATPAPRGSTAAHADAGEAKGGTRSSKTAASQSETAAPRKRTATAPERTTTPP
VASTRESAGTMSSNKLLTEAEILKMSDKDYMNEAQLAFFKNRLEQLRDEILKNADQTTEHLRETVIVPDPADRATIEEEH
ALELRTRDRERKLLKKVEQSLQRIESGDYGWCEETGEPIGVPRLLARPTATLSLEAQQRRELRQKLFGD
>Mature_228_residues
TAATKTKESRSKTGSAAVETRKAAPAKATPAPRGSTAAHADAGEAKGGTRSSKTAASQSETAAPRKRTATAPERTTTPPV
ASTRESAGTMSSNKLLTEAEILKMSDKDYMNEAQLAFFKNRLEQLRDEILKNADQTTEHLRETVIVPDPADRATIEEEHA
LELRTRDRERKLLKKVEQSLQRIESGDYGWCEETGEPIGVPRLLARPTATLSLEAQQRRELRQKLFGD

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=119, Percent_Identity=49.5798319327731, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 25128; Mature: 24997

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATKTKESRSKTGSAAVETRKAAPAKATPAPRGSTAAHADAGEAKGGTRSSKTAASQS
CCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ETAAPRKRTATAPERTTTPPVASTRESAGTMSSNKLLTEAEILKMSDKDYMNEAQLAFFK
HHCCCCHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
NRLEQLRDEILKNADQTTEHLRETVIVPDPADRATIEEEHALELRTRDRERKLLKKVEQS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQRIESGDYGWCEETGEPIGVPRLLARPTATLSLEAQQRRELRQKLFGD
HHHHHCCCCCCHHHCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAATKTKESRSKTGSAAVETRKAAPAKATPAPRGSTAAHADAGEAKGGTRSSKTAASQS
CCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ETAAPRKRTATAPERTTTPPVASTRESAGTMSSNKLLTEAEILKMSDKDYMNEAQLAFFK
HHCCCCHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
NRLEQLRDEILKNADQTTEHLRETVIVPDPADRATIEEEHALELRTRDRERKLLKKVEQS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQRIESGDYGWCEETGEPIGVPRLLARPTATLSLEAQQRRELRQKLFGD
HHHHHCCCCCCHHHCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]