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The map label for this gene is bioF

Identifier: 94309060

GI number: 94309060

Start: 121685

End: 122905

Strand: Direct

Name: bioF

Synonym: Rmet_0115

Alternate gene names: 94309060

Gene position: 121685-122905 (Clockwise)

Preceding gene: 94309059

Following gene: 94309061

Centisome position: 3.1

GC content: 68.8

Gene sequence:

>1221_bases
ATGCTGCTTGATCACCTGAAACGCGCGGCCGCGGAACGCGATGCCCAGTCGCTGACGCGCCGTCGCCGCATTGCTCACAC
GGCCTGCGCGCCGCACCAGTCGGTGGGCGGGATCGACGCCACGCCCACGCCGCTGCTCACGTTCTGCAGCAACGACTACC
TGGGCCTGGCCAATCACGGCTCGATCGCGCACGCGCTGGCCGAAGGCGCGCATCGCTACGGTGCGGGCAGCGGGGCATCG
CACCTGGTCAGCGGACATTCGCTGGCGCATGCGCAGCTCGAGCGTGAACTGGCGCGCTGGCTGGCACCTTACATCCCACA
GGCGCAGGCGCTGTATTTCTGCACTGGCTACATGGCAAACCTGGCGGTGCTGACGTCGCTCGGCGCGACTGGTGCGACGC
TGTTCTGCGAATCGCTCAACCATGCCTCGCTGATCGACGGCGCGCGTCTGGCGCGTGCCGATGTCCAGCGCTACCCTCAT
GGTGATGCAGGGGCGCTTGACGCGCTGCTGGCGGCCAGCACGACCGCGCTGAAGCTGATCGTGACCGATAGCGTCTTCAG
CATGGATGGCGATATCGCACCGCTGGCGGCGCTGCTGGAAGTCGCCGAACGCCACGATGCATGGATCATCGTCGACGACG
CGCACGGCTTTGGCGTGCTCGGCGAGAACGGCCATGGTGTGCTCGAACACCTGGGCCTGCGTTCAGAGCGGCTGATCTAC
ATCGGCACGCTCGGCAAGGCCGCTGGCGTGGCGGGCGCGTTCGTCGCGGCGCACGAGACGATCGTCGAACACCTGATCAA
CACGGCGCGCCCGTACATCTACACCACGGCAGCACCGCCGGCGGTTGCCCACGCGCTGCTGACGAGTCTGGAAATCATCG
CTGGCGAGGAGGGGCGCTCCCGCCGGGCGCATCTGACCGGGCTGATCGCGCAACTGCGCGCGGGGCTCGCGGTGCTGGCT
GCCCAGGCCGGGTGGTCGCTCGGTGAGAGCGAGACCGCCATCCAGCCGCTGATCGTTGGCGACAATGCCGCCGCGCTGGC
GCTGTCGGCCGCCCTCGAAGCCGAAGGCATTCGCGTTGGGGCGATTCGCCCGCCGACGGTGCCTGCCGGTACCGCGCGGC
TGCGCATCACGCTGTCGGCATCGCACTCTGCAGCCGACGTGGCGCGACTGCTGGAGACCATCGCGCGCGCCGCGCCCCAA
CTGCACAAGGAGGCTGCATGA

Upstream 100 bases:

>100_bases
ATGCCGCCGTATGTTTTCGATGCCGAGCTGGCCAACTGGCTCGGTGAACAATTGCTGGACACGCTGCAGGCAGTGCTCGA
TCCGGCGGAGGAGGCACGCC

Downstream 100 bases:

>100_bases
CCGGCATCGCCGACCGGCCAGAGCGCTTCGCCTGCTTCATCACCGGTACCGATACCGGCGTGGGCAAGACGCATGCGACC
GCCACGCTGCTGCACGCGCT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHGSIAHALAEGAHRYGAGSGAS
HLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMANLAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPH
GDAGALDALLAASTTALKLIVTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIY
IGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRSRRAHLTGLIAQLRAGLAVLA
AQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVGAIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQ
LHKEAA

Sequences:

>Translated_406_residues
MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHGSIAHALAEGAHRYGAGSGAS
HLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMANLAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPH
GDAGALDALLAASTTALKLIVTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIY
IGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRSRRAHLTGLIAQLRAGLAVLA
AQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVGAIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQ
LHKEAA
>Mature_406_residues
MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHGSIAHALAEGAHRYGAGSGAS
HLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMANLAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPH
GDAGALDALLAASTTALKLIVTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIY
IGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRSRRAHLTGLIAQLRAGLAVLA
AQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVGAIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQ
LHKEAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=379, Percent_Identity=32.1899736147757, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI284448556, Length=373, Percent_Identity=32.171581769437, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI83977442, Length=353, Percent_Identity=34.2776203966006, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI83977440, Length=353, Percent_Identity=34.2776203966006, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI83977444, Length=353, Percent_Identity=34.2776203966006, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4502025, Length=351, Percent_Identity=32.4786324786325, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI40316939, Length=351, Percent_Identity=32.4786324786325, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=27.0949720670391, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI119220554, Length=363, Percent_Identity=24.2424242424242, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5454084, Length=247, Percent_Identity=22.6720647773279, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786993, Length=349, Percent_Identity=46.1318051575931, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790046, Length=391, Percent_Identity=34.2710997442455, Blast_Score=214, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71994529, Length=368, Percent_Identity=29.8913043478261, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32566772, Length=371, Percent_Identity=27.4932614555256, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560912, Length=385, Percent_Identity=24.9350649350649, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=24.9350649350649, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982625, Length=270, Percent_Identity=22.2222222222222, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982617, Length=270, Percent_Identity=22.2222222222222, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320438, Length=372, Percent_Identity=30.6451612903226, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320267, Length=375, Percent_Identity=26.9333333333333, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323954, Length=252, Percent_Identity=25.7936507936508, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24662918, Length=378, Percent_Identity=32.2751322751323, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17137420, Length=384, Percent_Identity=30.7291666666667, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI17136286, Length=374, Percent_Identity=25.4010695187166, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24653280, Length=282, Percent_Identity=23.0496453900709, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653276, Length=282, Percent_Identity=23.0496453900709, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653278, Length=282, Percent_Identity=23.0496453900709, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_RALME (Q1LS75)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582270.1
- ProteinModelPortal:   Q1LS75
- STRING:   Q1LS75
- GeneID:   4036899
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0115
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   KDYASND
- PhylomeDB:   Q1LS75
- ProtClustDB:   PRK05958
- BioCyc:   RMET266264:RMET_0115-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42229; Mature: 42229

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 20-20 BINDING 141-141 BINDING 187-187 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEECCCCC
SIAHALAEGAHRYGAGSGASHLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMAN
HHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPHGDAGALDALLAASTTALKLI
HHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEE
VTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIY
EECCHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCHHHHHCCCCCCCEEE
IGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRS
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
RRAHLTGLIAQLRAGLAVLAAQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCEEEC
AIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQLHKEAA
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEECCCCC
SIAHALAEGAHRYGAGSGASHLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMAN
HHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPHGDAGALDALLAASTTALKLI
HHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEE
VTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIY
EECCHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCHHHHHCCCCCCCEEE
IGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRS
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
RRAHLTGLIAQLRAGLAVLAAQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCEEEC
AIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQLHKEAA
CCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA