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The map label for this gene is lipA [H]

Identifier: 94309006

GI number: 94309006

Start: 69085

End: 70077

Strand: Direct

Name: lipA [H]

Synonym: Rmet_0061

Alternate gene names: 94309006

Gene position: 69085-70077 (Clockwise)

Preceding gene: 94309005

Following gene: 94309007

Centisome position: 1.76

GC content: 63.44

Gene sequence:

>993_bases
ATGAGCGACGCCCCGATCGCCACGTCCAGCGAAGTCACGCAATCCCCTGCCGACTACGATCCGACCAAGAAGCAGAAGTC
GGCCGAGAAGACCGCTCGCATCCCTATCAAGATCGTGCCGGCGGAAAAGCTCAAGAAGCCGGACTGGATTCGCGTCAAGG
CTGCCACTGGCAATTCGCGCTTCTATGAGATCAAGGACATCCTGCGCGCCAACAATCTCGTGACCGTGTGCGAGGAAGCC
AGCTGCCCGAACATCGGTGAATGCTTCGGCAAGGGCACCGCCACGTTCATGATCATGGGTGACAAGTGCACGCGTCGCTG
CCCGTTCTGCGACGTGGGCCACGGCCGCCCGGACCCGCTCGACGTGAACGAGCCCGGCAACCTGGCGCGCACGATCGCCC
AGCTCAAGCTCAACTACGTGGTGATCACCAGCGTCGACCGCGACGACCTGCGTGACGGTGGCGCCCAGCACTACGTCGAC
TGCATCTCGCAAACGCGCGAGCTGTCGCCGAACACGCGTATCGAAGTGCTGGTGCCGGACTTCCGCGGCCGCCTGGACCG
CGCGCTGGACATCCTGCAGGCATGCCCGCCCGACGTGATGAACCACAACATGGAAACCGTGCCGCGCCTGTACAAGCAGG
CCCGCCCCGGCGCTGATTACGCGCATTCGCTGAAGCTGCTGCAGGATTTCAAGCGCCGCAATCCCAATGTGCCGACCAAG
TCGGGCCTGATGGTGGGCCTGGGCGAAACTGACGAGGAAATCCTCGACGTCATGCGCGACATGCGCGCCCACGACATCGA
CATGCTGACGATCGGCCAGTACCTGGCGCCGTCGAACCACCACCTGCCGGTTACGCGCTACGTCCACCCGGACACCTTCA
AGATGTTCGAAGAAGAGGCGTACAAGATGGGCTTCACCCACGCCGCTGTCGGCGCGATGGTCCGCAGCTCGTATCACGCT
GATCAGCAGGCGCATCAGGCGGGGTTTGCCTGA

Upstream 100 bases:

>100_bases
CAAGCCGCTGCCGAGGCCGCCTGAGCATGGCTAGCCCGGCCCGAATCACAGCCTATATTTACAGCCTACATTGCACGCTG
ACGCGCGTGCGGAGAACAGT

Downstream 100 bases:

>100_bases
TCGATCGACGTTTTCTTGCGCGTCACGAAAAGCGGCCGGATTTCCCGGCCGCTTTTTTTTGGCCCGTACGTAGCCACACC
CTTTCAGCAGCCTTTCCATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEEA
SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVD
CISQTRELSPNTRIEVLVPDFRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTK
SGLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYHA
DQQAHQAGFA

Sequences:

>Translated_330_residues
MSDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEEA
SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVD
CISQTRELSPNTRIEVLVPDFRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTK
SGLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYHA
DQQAHQAGFA
>Mature_329_residues
SDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEEAS
CPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDC
ISQTRELSPNTRIEVLVPDFRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTKS
GLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYHAD
QQAHQAGFA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=45.2380952380952, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI37577164, Length=256, Percent_Identity=46.09375, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=64.8026315789474, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=44.140625, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=43.9862542955326, Blast_Score=245, Evalue=9e-66,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.875, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36884; Mature: 36753

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSR
CCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECCCCC
FYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPL
HHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCC
DVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDCISQTRELSPNTRIEVLVPD
CCCCCHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCC
FRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTK
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
SGLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEA
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHHHHH
YKMGFTHAAVGAMVRSSYHADQQAHQAGFA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSR
CCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEECCCCC
FYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPL
HHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCC
DVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDCISQTRELSPNTRIEVLVPD
CCCCCHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCC
FRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTK
HHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
SGLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEA
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCEEEEECCHHHHHHHHHH
YKMGFTHAAVGAMVRSSYHADQQAHQAGFA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA