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The map label for this gene is lipB [H]

Identifier: 94309005

GI number: 94309005

Start: 68214

End: 69008

Strand: Direct

Name: lipB [H]

Synonym: Rmet_0060

Alternate gene names: 94309005

Gene position: 68214-69008 (Clockwise)

Preceding gene: 94309004

Following gene: 94309006

Centisome position: 1.74

GC content: 67.55

Gene sequence:

>795_bases
ATGACGAAGCAAATCGGGCCGGCCCGCGCTCTGCGTAGGCCGGCCCTTTTCGTTACACTCCTGCCCATGCATCCGATTCA
CGTCATCGACCGGGGCCGCCAGCCGTATCAGCCCTGTTTCGACGCCATGCGGGACTTCACCGCCACCCGCACGCCCGAGA
CGCCCGACCAGATCTGGCTGGTCGAGCATCCGCCGGTCTACACGCTTGGCCAGGCCGGCGACCCCGCGCATCTGCTCGCC
CCCGACGACACGATTCCGGTCGTGCAGATCGATCGTGGCGGCCAGATCACATACCACGGACCGGGACAGGTCGTGGCCTA
CCTGCTGCTCGATTTGCGCCGGCGTCACCTGATGGTGCGCGAGCTCGTGCACGACATCGAGCAGGCGGTACTGGACACGC
TCGCGGCGTATAATCTCGCAGCCGAACGCAAGGCCGGCGCCCCGGGTATCTACCTGTCCGACGGTAAGCACCAGGGTGCC
AAGATCGCCGCGCTTGGTCTCAAGATCCGCAACGGTTGCAGCTATCACGGCGTCAGCCTCAACGTGCAGATGGACCTGTC
GCCGTTCCTGCGCATCAATCCCTGCGGCTATGCCGGACTGGAAACGGTCGACATGGCTACCGCGGGCGCCGCCTTCACGG
CGGCGGACGATGCCGGCGGCGCGGTGCATCGGCCCGTCACCGCGACACAACAACCCGAGATCGCACAACGCCTGGCGGCG
GCCCTGTGCGAGGTACTGGCGGCACGCGAAGCGCGTGCCCCGGTGCCGGGACAAGCCGCTGCCGAGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCGGAAGACAAGATCACCGCCTCCTGATGCTGATGGTGCGGTGCCGGAGCGGCGGCTCAGACTTGTCTGGCCGCCGCGC
GACGAACCCTGAACACCAGC

Downstream 100 bases:

>100_bases
GCATGGCTAGCCCGGCCCGAATCACAGCCTATATTTACAGCCTACATTGCACGCTGACGCGCGTGCGGAGAACAGTATGA
GCGACGCCCCGATCGCCACG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWLVEHPPVYTLGQAGDPAHLLA
PDDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVRELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGA
KIAALGLKIRNGCSYHGVSLNVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAA
ALCEVLAAREARAPVPGQAAAEAA

Sequences:

>Translated_264_residues
MTKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWLVEHPPVYTLGQAGDPAHLLA
PDDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVRELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGA
KIAALGLKIRNGCSYHGVSLNVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAA
ALCEVLAAREARAPVPGQAAAEAA
>Mature_263_residues
TKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWLVEHPPVYTLGQAGDPAHLLAP
DDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVRELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGAK
IAALGLKIRNGCSYHGVSLNVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAAA
LCEVLAAREARAPVPGQAAAEAA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=175, Percent_Identity=58.8571428571429, Blast_Score=212, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323268, Length=172, Percent_Identity=30.8139534883721, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=178, Percent_Identity=30.8988764044944, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28335; Mature: 28204

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWL
CCCCCCHHHHHHCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
VEHPPVYTLGQAGDPAHLLAPDDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVR
EECCCEEEECCCCCCCEEECCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHH
ELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGAKIAALGLKIRNGCSYHGVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCEEE
NVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAA
EEEECCCCCEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCEECCCCCCCCHHHHHHHHH
ALCEVLAAREARAPVPGQAAAEAA
HHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
TKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWL
CCCCCHHHHHHCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
VEHPPVYTLGQAGDPAHLLAPDDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVR
EECCCEEEECCCCCCCEEECCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHH
ELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGAKIAALGLKIRNGCSYHGVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCEEE
NVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAA
EEEECCCCCEEECCCCCCCCCHHHHHHCCCEEEECCCCCCCEECCCCCCCCHHHHHHHHH
ALCEVLAAREARAPVPGQAAAEAA
HHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA