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The map label for this gene is bioB [H]

Identifier: 335055478

GI number: 335055478

Start: 123668

End: 124744

Strand: Direct

Name: bioB [H]

Synonym: NA

Alternate gene names: 335055478

Gene position: 123668-124744 (Clockwise)

Preceding gene: 94309061

Following gene: 94309063

Centisome position: 3.15

GC content: 65.74

Gene sequence:

>1077_bases
GTGGCCAAACCGAATCCACTACCGAACCCCATACCGATCATGACGCAACAAGCCAACCAGACCGTCGCCACCATTTCCGC
CGAAGCCCTGCGCCAGTCGGCCCGCAATATCGCGGCCGCCGCGCCGAAGGAGGGCGATGCATGGCGTGTCGACGATGTCG
CCGCACTGTTCGCACTGCCGTTCAACGACCTGCTGTTCCGCGCCCAGCAAGTGCATCGCGAGCACTTCGATGCCAACACC
GTGCAGCTTTCCACGCTGCTGTCGATCAAGACTGGTGGTTGCGAGGAGGACTGCGGCTACTGCCCGCAGAGCGCCCACCA
CGATGCGGGCGTGAAGGCTGAAAAGCTGATGGACCTTGAGGCCGTGCTCGACGCCGCCAAGGCGGCCAAGGCCAACGGCG
CCACGCGCTTCTGCATGGGCGCGGCATGGCGCGAGCCGAAGGAACGCCACCTCGAGCCCGTGATCGACATGGTGCGCGAG
GTCAAGGCGATGGGCCTGGAAACCTGCGTGACGCTGGGGATGCTCAAGGCCGAACAGGCGCAGCGTCTGAAGGACGCGGG
CCTCGACTACTACAACCACAACCTCGATACCTCGCCCGAGTTCTACGGCAAGATCATCACCACGCGCACGTACCAGGATC
GGCTGGACACCATCGGCCATGTGCGCGAAGCCGGCATCAACGTCTGCTGTGGCGGCATCGTCGGCATGGGCGAAGCCCGA
GAGGCGCGTGCCGGCCTGATCGCGCAACTGGCCAATATGGACCCGTACCCGGAGTCGGTGCCCATCAACAACCTGGTACA
GGTGGAAGGCACGCCGCTGGCGGGCACCGAGGCGCTGGACCCGTTCGAGTTTGTCCGCACGATCGCGGTGGCGCGTATCA
CGATGCCGCGCGCGATGGTGCGTCTGTCCGCTGGCCGCGAAGCCATGGATGAGGCGCTGCAGGCACTGTGCTTCATGGCC
GGCGCGAACTCGATCTTCTACGGCGAGAAGCTGCTGACCACCGGCAATCCCCAGGCTGACCGCGACCGCGCGCTGCTGGC
GCGTCTGGATATCCGCGCCGAGGGCTACGCGGGGTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: biotin synthase slightly down RPM proteomics BL (0,72)down also RWV BL (0.68)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:
NA

Sequences:

>Translated_358_residues
MAKPNPLPNPIPIMTQQANQTVATISAEALRQSARNIAAAAPKEGDAWRVDDVAALFALPFNDLLFRAQQVHREHFDANT
VQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMDLEAVLDAAKAAKANGATRFCMGAAWREPKERHLEPVIDMVRE
VKAMGLETCVTLGMLKAEQAQRLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVREAGINVCCGGIVGMGEAR
EARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMVRLSAGREAMDEALQALCFMA
GANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG
>Mature_357_residues
AKPNPLPNPIPIMTQQANQTVATISAEALRQSARNIAAAAPKEGDAWRVDDVAALFALPFNDLLFRAQQVHREHFDANTV
QLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMDLEAVLDAAKAAKANGATRFCMGAAWREPKERHLEPVIDMVREV
KAMGLETCVTLGMLKAEQAQRLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVREAGINVCCGGIVGMGEARE
ARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMVRLSAGREAMDEALQALCFMAG
ANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=310, Percent_Identity=63.8709677419355, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321725, Length=358, Percent_Identity=47.7653631284916, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38868; Mature: 38737

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPNPLPNPIPIMTQQANQTVATISAEALRQSARNIAAAAPKEGDAWRVDDVAALFALP
CCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCC
FNDLLFRAQQVHREHFDANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMDLE
HHHHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHH
AVLDAAKAAKANGATRFCMGAAWREPKERHLEPVIDMVREVKAMGLETCVTLGMLKAEQA
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVREAGINVCCGGIVGMGEAR
HHHHHCCCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCHHCCCCHH
EARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMV
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG
HHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKPNPLPNPIPIMTQQANQTVATISAEALRQSARNIAAAAPKEGDAWRVDDVAALFALP
CCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCC
FNDLLFRAQQVHREHFDANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMDLE
HHHHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHH
AVLDAAKAAKANGATRFCMGAAWREPKERHLEPVIDMVREVKAMGLETCVTLGMLKAEQA
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVREAGINVCCGGIVGMGEAR
HHHHHCCCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCHHCCCCHH
EARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMV
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG
HHHCCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA