Click here to switch to the map view.

The map label for this gene is ycdW [H]

Identifier: 94309063

GI number: 94309063

Start: 124894

End: 125835

Strand: Direct

Name: ycdW [H]

Synonym: Rmet_0118

Alternate gene names: 94309063

Gene position: 124894-125835 (Clockwise)

Preceding gene: 335055478

Following gene: 94309064

Centisome position: 3.18

GC content: 66.45

Gene sequence:

>942_bases
ATGAAGTTGCAGCTGTACGTGCCGGATGGCCGCTATGCCCCGTGGATCGATGGTTTCGCCGAAGTACTGCCGGAAGCGCA
GTGCGTGACCTGGGAAGACAGCCAGGGCCAGTCCGTGGACTACGCAGTGGTGTGGCGTCCGCCGGTGGAAATGCTGCGCG
GCCGTACCGATCTCAAGGCCATCTTCAATCTCGGCGCTGGTGTCGACGGTATCCTGAAGCTGAATGCCCAGCATCCCGAT
GCCCTGCCGGCCGGCGTGCCGATCATCCGGCTCGATGACGCCGGCATGGCGGCCCAGATGGCCGAGTACGTCACGGCCGC
CGTGCTGCGTTACTTCCGCCGGCTCGATGTGTACGACGCGCAGGAACGCGCCGGCACGTGGAAATTCCAGAAACCGCATC
GTCGTGCCGATTTCACGATCGGCGTGATGGGTGTTGGCACGCTCGGCGCCCATATCGCCCGCACGCTGGCTGGCTTCGGC
TTCCCGGTGCGCGGATGGAGCCGCTCGCCGAAGACGGTGGACGGCGTGCAATCGTTCCACGGTGAAGACGGGCAGGCCGC
CTTCCTCGATGGCTTGCGCGTGCTGGTCAACGTGCTGCCGCTGACGCCCGAGACCCAGGACATCATCGACACGGGCCTGC
TGCATCGCCTGGCGCACGGCGCCTACCTGATCAACGTGGCGCGCGGCCAGCACCTCGTGGAAGAGGATCTGCTCGCCGCC
GTGCGTGAAGGACAGATCGCGGGTGCCACGCTTGACGTGTTCCGCACCGAGCCGCTGCCTGCCGATCATCCATACTGGCA
GGAGCCGCGCATCACGATCACGCCGCACATCTCGGCGCTGACGCTGCGCGAGGACAGCATTGCGCAGATCGCCGGCAAGA
TCCGCCTGCTCGAGTCGGGCAAGCCGATCGCCGGCGTGGTGGACCTGCAGCGCGGCTACTAG

Upstream 100 bases:

>100_bases
CGATCCGCCATGCGGAACGCGCTTCCGCAATCCAACCGCTGGCCCGCGACCTCTGCCAAAATACTTCATCCCATCCCCCA
AAAAGAAAACTGGAGTCGAG

Downstream 100 bases:

>100_bases
ACCGCGACGCGCCCAGACAAAGGAGACACGATCCATGAGCGAGAGCCCCACGCTTCCCGATGCCGCGCAACGCGTGGCCG
ATCTGTTGGTCGGCATCGGC

Product: 2-ketoacid reductase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLQLYVPDGRYAPWIDGFAEVLPEAQCVTWEDSQGQSVDYAVVWRPPVEMLRGRTDLKAIFNLGAGVDGILKLNAQHPD
ALPAGVPIIRLDDAGMAAQMAEYVTAAVLRYFRRLDVYDAQERAGTWKFQKPHRRADFTIGVMGVGTLGAHIARTLAGFG
FPVRGWSRSPKTVDGVQSFHGEDGQAAFLDGLRVLVNVLPLTPETQDIIDTGLLHRLAHGAYLINVARGQHLVEEDLLAA
VREGQIAGATLDVFRTEPLPADHPYWQEPRITITPHISALTLREDSIAQIAGKIRLLESGKPIAGVVDLQRGY

Sequences:

>Translated_313_residues
MKLQLYVPDGRYAPWIDGFAEVLPEAQCVTWEDSQGQSVDYAVVWRPPVEMLRGRTDLKAIFNLGAGVDGILKLNAQHPD
ALPAGVPIIRLDDAGMAAQMAEYVTAAVLRYFRRLDVYDAQERAGTWKFQKPHRRADFTIGVMGVGTLGAHIARTLAGFG
FPVRGWSRSPKTVDGVQSFHGEDGQAAFLDGLRVLVNVLPLTPETQDIIDTGLLHRLAHGAYLINVARGQHLVEEDLLAA
VREGQIAGATLDVFRTEPLPADHPYWQEPRITITPHISALTLREDSIAQIAGKIRLLESGKPIAGVVDLQRGY
>Mature_313_residues
MKLQLYVPDGRYAPWIDGFAEVLPEAQCVTWEDSQGQSVDYAVVWRPPVEMLRGRTDLKAIFNLGAGVDGILKLNAQHPD
ALPAGVPIIRLDDAGMAAQMAEYVTAAVLRYFRRLDVYDAQERAGTWKFQKPHRRADFTIGVMGVGTLGAHIARTLAGFG
FPVRGWSRSPKTVDGVQSFHGEDGQAAFLDGLRVLVNVLPLTPETQDIIDTGLLHRLAHGAYLINVARGQHLVEEDLLAA
VREGQIAGATLDVFRTEPLPADHPYWQEPRITITPHISALTLREDSIAQIAGKIRLLESGKPIAGVVDLQRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=264, Percent_Identity=28.7878787878788, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI23308577, Length=288, Percent_Identity=26.7361111111111, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=25.6916996047431, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=25.6916996047431, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=48.2428115015974, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=27.8884462151394, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=235, Percent_Identity=24.6808510638298, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571528, Length=178, Percent_Identity=30.3370786516854, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34370; Mature: 34370

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3 ; PS00729 AP_NUCLEASE_F2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQLYVPDGRYAPWIDGFAEVLPEAQCVTWEDSQGQSVDYAVVWRPPVEMLRGRTDLKA
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHCCCHHHHH
IFNLGAGVDGILKLNAQHPDALPAGVPIIRLDDAGMAAQMAEYVTAAVLRYFRRLDVYDA
HHHCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
QERAGTWKFQKPHRRADFTIGVMGVGTLGAHIARTLAGFGFPVRGWSRSPKTVDGVQSFH
HHHCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
GEDGQAAFLDGLRVLVNVLPLTPETQDIIDTGLLHRLAHGAYLINVARGQHLVEEDLLAA
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VREGQIAGATLDVFRTEPLPADHPYWQEPRITITPHISALTLREDSIAQIAGKIRLLESG
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHEEEEECC
KPIAGVVDLQRGY
CCEEEEEECCCCC
>Mature Secondary Structure
MKLQLYVPDGRYAPWIDGFAEVLPEAQCVTWEDSQGQSVDYAVVWRPPVEMLRGRTDLKA
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCHHHHCCCHHHHH
IFNLGAGVDGILKLNAQHPDALPAGVPIIRLDDAGMAAQMAEYVTAAVLRYFRRLDVYDA
HHHCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
QERAGTWKFQKPHRRADFTIGVMGVGTLGAHIARTLAGFGFPVRGWSRSPKTVDGVQSFH
HHHCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
GEDGQAAFLDGLRVLVNVLPLTPETQDIIDTGLLHRLAHGAYLINVARGQHLVEEDLLAA
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VREGQIAGATLDVFRTEPLPADHPYWQEPRITITPHISALTLREDSIAQIAGKIRLLESG
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHEEEEECC
KPIAGVVDLQRGY
CCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA