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The map label for this gene is sbp [H]

Identifier: 93006216

GI number: 93006216

Start: 1699193

End: 1700290

Strand: Reverse

Name: sbp [H]

Synonym: Pcryo_1390

Alternate gene names: 93006216

Gene position: 1700290-1699193 (Counterclockwise)

Preceding gene: 93006217

Following gene: 93006215

Centisome position: 55.57

GC content: 44.35

Gene sequence:

>1098_bases
ATGACATCATATACCGCAGGTTATAAAAATAAGACACTTAGCGCCCTAAGTATCGCCGGTGTGGCGCTCACTTTAGGTAT
TGCAGGCTGTAGCAACAACGAAAAAACTGAGCCAGCTGCCAATGCAGAGGGCACGCCAGCAGCGACAGAAGGGCAGGACA
TCGAAATATTGAACGTCTCCTACGATGTCGCCCGTGATTTTTATAAAGACTACAATCCATTATTTGTTGATCATTACAAA
AGCGAGCACCCAAACAGCAATATTCTTATCAAGCAGTCGCATGGTGGTTCGAGTAAACAAGCCCTGTCGGTCGCGAATGG
TTTACAAGCCGATGTCGCAACCATGAACCAAGGCTCTGATATTGAGCTGCTTGAGAAAAAAGGCTTGGTTGAGTCAGATT
GGGAAAGCAAATTCCCAGACAATGCCGTACCTTTTACCAGTACCATCGTCTTTTTAGTGCGCAAAGACAATCCAAAAGGC
ATCAATGATTGGGCAGATTTGACCAAAGAGGGTGTTGAGATTGTCATGGCAAACCCAAAAGTGACGGGTAACGGTCGCTA
TGCCTTCTTAGGTGCTTATGGTTATGGTTTACATGCTTTTAATAAAGATGAAGCACAAGCAGAAAAATACGTCAAAGACA
TGCTAAAAAACGTCAAAGTCTATGAAAGTGGCGGACGTGCGGCGACTACGACGTTTGTACAACGTGGTATCGGTGATGTC
TTGGTAACATTTGAGAACGAAGCCAACCTTGCGGCAAAAGATTTTGGCGTCGGTCAAGTCGATATTGTTTATCCAAAATA
CTCAATAAAATCTGAAAGCCCAGTTGCTATTGTAAAAACCGTCACTGATAAAAAAGGCACCACAGATGCCGCCAAAGCCT
ATCTTGATTTCTTATGGAGCGATGCAGCCCAGCAATTGGCAGCTGACTTATACTTGCGCCCAAGTGTGAAGAGTGTTCTT
GATAAAAACAGTGATAAATTGCCACCTATCGACACCTTCCGTCCGAATGACGCTTTTGGCACTTGGGATGAGATCATGAG
CACCTATTTCAGTGATGGTGGTTTGTTTGATAAGTTAGCGGTTAATGCGCCTCAGTAA

Upstream 100 bases:

>100_bases
TTCCATTTTGGCAATAACATACACATATTCATCATTAAACATAATAAGATAGCACTGTTAGCATACTTACATTACTTATC
AGACATTTTTTGAGGCAGCC

Downstream 100 bases:

>100_bases
CAACGGTAGTACTGTGAGTAACCGACGATAAAAGCCGTTACTTATTCAAGAATTTATTTGAGTACAGCGACTGCATTATT
TGACTATTAATCAGTAACGC

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTSYTAGYKNKTLSALSIAGVALTLGIAGCSNNEKTEPAANAEGTPAATEGQDIEILNVSYDVARDFYKDYNPLFVDHYK
SEHPNSNILIKQSHGGSSKQALSVANGLQADVATMNQGSDIELLEKKGLVESDWESKFPDNAVPFTSTIVFLVRKDNPKG
INDWADLTKEGVEIVMANPKVTGNGRYAFLGAYGYGLHAFNKDEAQAEKYVKDMLKNVKVYESGGRAATTTFVQRGIGDV
LVTFENEANLAAKDFGVGQVDIVYPKYSIKSESPVAIVKTVTDKKGTTDAAKAYLDFLWSDAAQQLAADLYLRPSVKSVL
DKNSDKLPPIDTFRPNDAFGTWDEIMSTYFSDGGLFDKLAVNAPQ

Sequences:

>Translated_365_residues
MTSYTAGYKNKTLSALSIAGVALTLGIAGCSNNEKTEPAANAEGTPAATEGQDIEILNVSYDVARDFYKDYNPLFVDHYK
SEHPNSNILIKQSHGGSSKQALSVANGLQADVATMNQGSDIELLEKKGLVESDWESKFPDNAVPFTSTIVFLVRKDNPKG
INDWADLTKEGVEIVMANPKVTGNGRYAFLGAYGYGLHAFNKDEAQAEKYVKDMLKNVKVYESGGRAATTTFVQRGIGDV
LVTFENEANLAAKDFGVGQVDIVYPKYSIKSESPVAIVKTVTDKKGTTDAAKAYLDFLWSDAAQQLAADLYLRPSVKSVL
DKNSDKLPPIDTFRPNDAFGTWDEIMSTYFSDGGLFDKLAVNAPQ
>Mature_364_residues
TSYTAGYKNKTLSALSIAGVALTLGIAGCSNNEKTEPAANAEGTPAATEGQDIEILNVSYDVARDFYKDYNPLFVDHYKS
EHPNSNILIKQSHGGSSKQALSVANGLQADVATMNQGSDIELLEKKGLVESDWESKFPDNAVPFTSTIVFLVRKDNPKGI
NDWADLTKEGVEIVMANPKVTGNGRYAFLGAYGYGLHAFNKDEAQAEKYVKDMLKNVKVYESGGRAATTTFVQRGIGDVL
VTFENEANLAAKDFGVGQVDIVYPKYSIKSESPVAIVKTVTDKKGTTDAAKAYLDFLWSDAAQQLAADLYLRPSVKSVLD
KNSDKLPPIDTFRPNDAFGTWDEIMSTYFSDGGLFDKLAVNAPQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=309, Percent_Identity=45.6310679611651, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1788765, Length=309, Percent_Identity=45.9546925566343, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39622; Mature: 39490

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYTAGYKNKTLSALSIAGVALTLGIAGCSNNEKTEPAANAEGTPAATEGQDIEILNVS
CCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECH
YDVARDFYKDYNPLFVDHYKSEHPNSNILIKQSHGGSSKQALSVANGLQADVATMNQGSD
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHCCCCCC
IELLEKKGLVESDWESKFPDNAVPFTSTIVFLVRKDNPKGINDWADLTKEGVEIVMANPK
CHHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCE
VTGNGRYAFLGAYGYGLHAFNKDEAQAEKYVKDMLKNVKVYESGGRAATTTFVQRGIGDV
EECCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEE
LVTFENEANLAAKDFGVGQVDIVYPKYSIKSESPVAIVKTVTDKKGTTDAAKAYLDFLWS
EEEEECCCCCEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
DAAQQLAADLYLRPSVKSVLDKNSDKLPPIDTFRPNDAFGTWDEIMSTYFSDGGLFDKLA
HHHHHHHHHHEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHC
VNAPQ
CCCCC
>Mature Secondary Structure 
TSYTAGYKNKTLSALSIAGVALTLGIAGCSNNEKTEPAANAEGTPAATEGQDIEILNVS
CCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECH
YDVARDFYKDYNPLFVDHYKSEHPNSNILIKQSHGGSSKQALSVANGLQADVATMNQGSD
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHCCCCCC
IELLEKKGLVESDWESKFPDNAVPFTSTIVFLVRKDNPKGINDWADLTKEGVEIVMANPK
CHHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCE
VTGNGRYAFLGAYGYGLHAFNKDEAQAEKYVKDMLKNVKVYESGGRAATTTFVQRGIGDV
EECCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEE
LVTFENEANLAAKDFGVGQVDIVYPKYSIKSESPVAIVKTVTDKKGTTDAAKAYLDFLWS
EEEEECCCCCEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
DAAQQLAADLYLRPSVKSVLDKNSDKLPPIDTFRPNDAFGTWDEIMSTYFSDGGLFDKLA
HHHHHHHHHHEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHC
VNAPQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]