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The map label for this gene is cysU [H]

Identifier: 93006215

GI number: 93006215

Start: 1698042

End: 1698926

Strand: Reverse

Name: cysU [H]

Synonym: Pcryo_1389

Alternate gene names: 93006215

Gene position: 1698926-1698042 (Counterclockwise)

Preceding gene: 93006216

Following gene: 93006214

Centisome position: 55.52

GC content: 47.01

Gene sequence:

>885_bases
ATGAGTGCAACAACACCTTCTACCATCAAACCCGTGCCGAAAAAAGGCTGGTTAACACGCTTGCGTCAGCGCAATGTGCT
ACCAGGGTTTGGGCTAAGTATGGGTATCACTGTCTTTAGTTTGTCACTGTTGGTAGTACTGCCGTTTGCCATGATGGCCT
ATACCACCACACAAATGGGCTGGACGGGATTTTGGGAGACTATTAGCCAACCACAAGTCACGGCAGCGATCAAACTCAGC
TTATGGATGTCATTTTTGGCGATGCTAACCAATATGGTGTTTGGCACACTGGTCGCATGGGTGCTCGTACGCTATGAATT
TTGGGGCAAATCCTTGATTAATGCCCTAGTGGATTTACCCTTTGCACTACCGACAGCGGTTACTGGTATCTCTCTTGCGA
CTTTGTATGCACCTAATGGTCTTATCGGGCAATGGTTTGCCAAGTTTGATATTCAAGTGGCATTTACACCGATTGGTATT
TGGTTGGCTTTGGTGGTCGTCAGTTTTCCCTTTATTGTTCGTGCTGTACAGCCCGTACTTGCGGAGTTATCGGTTGAGTT
CGAAGAGGCGTCTGCGGTATTGGGTGCCAATCGCTTGACGACATTTCGCAAGGTAATTTTACCAGAGCTGTTGCCTGCGT
TACTGATGGGTGCTGGCATGATGTTTGCCCGTGCGACCGGCGAGTATGGCTCGGTTATTTTTATCGCAGGTAATATTCCA
ATGCAGTCTGAGATTTTGCCATTAATCATTATCAGTAAGCTTGAGCAGTTTGATGTTCAAGGCGCGTCAGCCGTTGCATT
ATTTATGCTGCTTATCTCCTTTGTGATTTTATTGACCATTAATATCGTACAGTGGAAGCTGTCGCGCCGTGTAGGAGCTC
GCTAA

Upstream 100 bases:

>100_bases
TGAGCATATTCATCATCTCTGTTTATCATACTTTTCTCTTTATATCGATACGATAGAGTGCTTACGATAAGTACCATCAG
CTAGGCAATAGGACACTATT

Downstream 100 bases:

>100_bases
TGCAAATCTCTAATAGCTACGACTACCAAAGCAACCCAGCAACCAAAGATACGCCATGGGTACGCCGCACCTTTATTATC
ATTGCGGTTTTATTCATGGT

Product: Sulfate ABC transporter, permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSATTPSTIKPVPKKGWLTRLRQRNVLPGFGLSMGITVFSLSLLVVLPFAMMAYTTTQMGWTGFWETISQPQVTAAIKLS
LWMSFLAMLTNMVFGTLVAWVLVRYEFWGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQWFAKFDIQVAFTPIGI
WLALVVVSFPFIVRAVQPVLAELSVEFEEASAVLGANRLTTFRKVILPELLPALLMGAGMMFARATGEYGSVIFIAGNIP
MQSEILPLIIISKLEQFDVQGASAVALFMLLISFVILLTINIVQWKLSRRVGAR

Sequences:

>Translated_294_residues
MSATTPSTIKPVPKKGWLTRLRQRNVLPGFGLSMGITVFSLSLLVVLPFAMMAYTTTQMGWTGFWETISQPQVTAAIKLS
LWMSFLAMLTNMVFGTLVAWVLVRYEFWGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQWFAKFDIQVAFTPIGI
WLALVVVSFPFIVRAVQPVLAELSVEFEEASAVLGANRLTTFRKVILPELLPALLMGAGMMFARATGEYGSVIFIAGNIP
MQSEILPLIIISKLEQFDVQGASAVALFMLLISFVILLTINIVQWKLSRRVGAR
>Mature_293_residues
SATTPSTIKPVPKKGWLTRLRQRNVLPGFGLSMGITVFSLSLLVVLPFAMMAYTTTQMGWTGFWETISQPQVTAAIKLSL
WMSFLAMLTNMVFGTLVAWVLVRYEFWGKSLINALVDLPFALPTAVTGISLATLYAPNGLIGQWFAKFDIQVAFTPIGIW
LALVVVSFPFIVRAVQPVLAELSVEFEEASAVLGANRLTTFRKVILPELLPALLMGAGMMFARATGEYGSVIFIAGNIPM
QSEILPLIIISKLEQFDVQGASAVALFMLLISFVILLTINIVQWKLSRRVGAR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=49.4423791821561, Blast_Score=251, Evalue=3e-68,
Organism=Escherichia coli, GI87082099, Length=276, Percent_Identity=29.3478260869565, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=34.5291479820628, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=27.1966527196653, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32264; Mature: 32133

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTPSTIKPVPKKGWLTRLRQRNVLPGFGLSMGITVFSLSLLVVLPFAMMAYTTTQMG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WTGFWETISQPQVTAAIKLSLWMSFLAMLTNMVFGTLVAWVLVRYEFWGKSLINALVDLP
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FALPTAVTGISLATLYAPNGLIGQWFAKFDIQVAFTPIGIWLALVVVSFPFIVRAVQPVL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AELSVEFEEASAVLGANRLTTFRKVILPELLPALLMGAGMMFARATGEYGSVIFIAGNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCC
MQSEILPLIIISKLEQFDVQGASAVALFMLLISFVILLTINIVQWKLSRRVGAR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SATTPSTIKPVPKKGWLTRLRQRNVLPGFGLSMGITVFSLSLLVVLPFAMMAYTTTQMG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WTGFWETISQPQVTAAIKLSLWMSFLAMLTNMVFGTLVAWVLVRYEFWGKSLINALVDLP
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FALPTAVTGISLATLYAPNGLIGQWFAKFDIQVAFTPIGIWLALVVVSFPFIVRAVQPVL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AELSVEFEEASAVLGANRLTTFRKVILPELLPALLMGAGMMFARATGEYGSVIFIAGNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCC
MQSEILPLIIISKLEQFDVQGASAVALFMLLISFVILLTINIVQWKLSRRVGAR
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]