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The map label for this gene is sbp [H]

Identifier: 93006217

GI number: 93006217

Start: 1700577

End: 1701746

Strand: Reverse

Name: sbp [H]

Synonym: Pcryo_1391

Alternate gene names: 93006217

Gene position: 1701746-1700577 (Counterclockwise)

Preceding gene: 93006219

Following gene: 93006216

Centisome position: 55.61

GC content: 45.9

Gene sequence:

>1170_bases
ATGACATTATCGAGAGCGTCTATGCAAACGACCAATATAGCCAAGAATCAGCCTTTGCGTCATACCTCAATGACTGATAA
TCAAGTGCTTCAAAATAAAGCACCACGTACCTTGATAATGGGTGGTGTGCTGACAATGGCGGTATTCATGACGGGCTGTA
GCCCTACTGAATCTAAGCAAAGTGAGACCCAAAACGGCGCCCAAAAATCAGCAGCGCCGTCACAAGATATTGATTTGTTG
AATGTCTCTTACGATGTCTCACGCGATTTTTATAAAGATTATAATACTTTATTTGCCAAAGGCTACCAAAAAGAGCATCC
CAACAGCAAAGTACATATCAATCAGTCACATGGTGGCTCGAGTAAGCAAGCCCTGTCGGTTGCTAATGGTCTACAAGCGG
ATGTGGTGACCTTTAACCAAGAAAGCGATATGAATCTATTGGTTGAAAAAGGCTTGGTTGCTGCTGACTGGCAGAAAGCG
TTACCAAACGGCGCGGTCCCTTATACCAGTACCATGGTTCTATTGGTACGCAGCGGCAATCCAAAGGGTATTAAAGACTG
GCAAGATTTGGCACGTAGTGACGTAGCTATAGTAATACCCAATCCGAAAACCAGTGGTACGGCGCGTTATGCCTTTTTAG
GTGCCTACGGTTATGGTCTGCATCATTTTAAAGAAACTGTCGAAACCTCTACCAAAACAGATACCTTTATCAAACAACTT
TTGGCAAACGTCGTCACTTATGACAATGGCGCGCGCGCCGCAACGACCAGCTTTACCCAACGCGGTCTAGGGGACGTGCT
TATCACGACCGAAAACGAAGCGCATTTGGCCGCCAAGCAATTTGCCAAAGGACAAGTCGATATCGTCTATCCAAGTTATT
CAGTGTTGATTGCCAATCCTGTTGCAGTGGTGAATGCGGTGACTGACAAAGCGGGCAAAACAGAAGCGGCAAACACCTAT
CTAAAGAGTTTATGGGACACGCCAGCGCAAGAACTGATGGCACAGATGTACATGCGTCCTAGTAATGAGCAAGTACTTGC
CGCCCATAAAGGTACTTTGCTTGATATTGAGACGTTTGAGCCAGTCAGTGTCTTTGGTCCTTGGACACAGATTATGGAGA
CGTTTTTTGTTGATGGCGGGCGTTTTGATAAGCTGGCAAGCGTGCAGTAA

Upstream 100 bases:

>100_bases
CAACAAAAAAACCAATGTATCTCCCAAAATTCTTACCATCACTGCCTTTGTTATCCTTATTTATAGAATTAATAAAATAG
TAAGGTCCACAAGTGGCAGC

Downstream 100 bases:

>100_bases
AGGTAATAAAAAAGGTAATCAAGTTGTTCAGTTATAAGCCATTTGATTCAGACATTCATGCTTAGATGAAAGGTTGAATC
AAATGGCTAAATTATTAGCG

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTLSRASMQTTNIAKNQPLRHTSMTDNQVLQNKAPRTLIMGGVLTMAVFMTGCSPTESKQSETQNGAQKSAAPSQDIDLL
NVSYDVSRDFYKDYNTLFAKGYQKEHPNSKVHINQSHGGSSKQALSVANGLQADVVTFNQESDMNLLVEKGLVAADWQKA
LPNGAVPYTSTMVLLVRSGNPKGIKDWQDLARSDVAIVIPNPKTSGTARYAFLGAYGYGLHHFKETVETSTKTDTFIKQL
LANVVTYDNGARAATTSFTQRGLGDVLITTENEAHLAAKQFAKGQVDIVYPSYSVLIANPVAVVNAVTDKAGKTEAANTY
LKSLWDTPAQELMAQMYMRPSNEQVLAAHKGTLLDIETFEPVSVFGPWTQIMETFFVDGGRFDKLASVQ

Sequences:

>Translated_389_residues
MTLSRASMQTTNIAKNQPLRHTSMTDNQVLQNKAPRTLIMGGVLTMAVFMTGCSPTESKQSETQNGAQKSAAPSQDIDLL
NVSYDVSRDFYKDYNTLFAKGYQKEHPNSKVHINQSHGGSSKQALSVANGLQADVVTFNQESDMNLLVEKGLVAADWQKA
LPNGAVPYTSTMVLLVRSGNPKGIKDWQDLARSDVAIVIPNPKTSGTARYAFLGAYGYGLHHFKETVETSTKTDTFIKQL
LANVVTYDNGARAATTSFTQRGLGDVLITTENEAHLAAKQFAKGQVDIVYPSYSVLIANPVAVVNAVTDKAGKTEAANTY
LKSLWDTPAQELMAQMYMRPSNEQVLAAHKGTLLDIETFEPVSVFGPWTQIMETFFVDGGRFDKLASVQ
>Mature_388_residues
TLSRASMQTTNIAKNQPLRHTSMTDNQVLQNKAPRTLIMGGVLTMAVFMTGCSPTESKQSETQNGAQKSAAPSQDIDLLN
VSYDVSRDFYKDYNTLFAKGYQKEHPNSKVHINQSHGGSSKQALSVANGLQADVVTFNQESDMNLLVEKGLVAADWQKAL
PNGAVPYTSTMVLLVRSGNPKGIKDWQDLARSDVAIVIPNPKTSGTARYAFLGAYGYGLHHFKETVETSTKTDTFIKQLL
ANVVTYDNGARAATTSFTQRGLGDVLITTENEAHLAAKQFAKGQVDIVYPSYSVLIANPVAVVNAVTDKAGKTEAANTYL
KSLWDTPAQELMAQMYMRPSNEQVLAAHKGTLLDIETFEPVSVFGPWTQIMETFFVDGGRFDKLASVQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=313, Percent_Identity=43.7699680511182, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=44.5512820512821, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42446; Mature: 42315

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSRASMQTTNIAKNQPLRHTSMTDNQVLQNKAPRTLIMGGVLTMAVFMTGCSPTESKQ
CCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCHHH
SETQNGAQKSAAPSQDIDLLNVSYDVSRDFYKDYNTLFAKGYQKEHPNSKVHINQSHGGS
HHHHCCHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCC
SKQALSVANGLQADVVTFNQESDMNLLVEKGLVAADWQKALPNGAVPYTSTMVLLVRSGN
CHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEHHHHHHCCCCCCCCCCEEEEEEECCC
PKGIKDWQDLARSDVAIVIPNPKTSGTARYAFLGAYGYGLHHFKETVETSTKTDTFIKQL
CCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
LANVVTYDNGARAATTSFTQRGLGDVLITTENEAHLAAKQFAKGQVDIVYPSYSVLIANP
HHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCEEEEECH
VAVVNAVTDKAGKTEAANTYLKSLWDTPAQELMAQMYMRPSNEQVLAAHKGTLLDIETFE
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCC
PVSVFGPWTQIMETFFVDGGRFDKLASVQ
CCEECCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TLSRASMQTTNIAKNQPLRHTSMTDNQVLQNKAPRTLIMGGVLTMAVFMTGCSPTESKQ
CCCCCCCCHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCHHH
SETQNGAQKSAAPSQDIDLLNVSYDVSRDFYKDYNTLFAKGYQKEHPNSKVHINQSHGGS
HHHHCCHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCC
SKQALSVANGLQADVVTFNQESDMNLLVEKGLVAADWQKALPNGAVPYTSTMVLLVRSGN
CHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEHHHHHHCCCCCCCCCCEEEEEEECCC
PKGIKDWQDLARSDVAIVIPNPKTSGTARYAFLGAYGYGLHHFKETVETSTKTDTFIKQL
CCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
LANVVTYDNGARAATTSFTQRGLGDVLITTENEAHLAAKQFAKGQVDIVYPSYSVLIANP
HHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCEEEEECH
VAVVNAVTDKAGKTEAANTYLKSLWDTPAQELMAQMYMRPSNEQVLAAHKGTLLDIETFE
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCC
PVSVFGPWTQIMETFFVDGGRFDKLASVQ
CCEECCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]